Search results for the GEO ID: GSE47205  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1146833 | GPL1261 |
|
Mll deficient HSC, biological rep1
|
Mll deficient LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mx1-cre;Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from Mll deficient HSCs
|
| Sample_geo_accession | GSM1146833
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146833/suppl/GSM1146833_PE022008_SampleT2753.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146833/suppl/GSM1146833_PE022008_SampleT2753.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146834 | GPL1261 |
|
Mll deficient HSC, biological rep2
|
Mll deficient LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mx1-cre;Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from Mll deficient HSCs
|
| Sample_geo_accession | GSM1146834
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146834/suppl/GSM1146834_PE022008_SampleT2754.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146834/suppl/GSM1146834_PE022008_SampleT2754.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146835 | GPL1261 |
|
Control HSC, biological rep1
|
Control LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from control HSCs
|
| Sample_geo_accession | GSM1146835
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146835/suppl/GSM1146835_PE022008_SampleT2755.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146835/suppl/GSM1146835_PE022008_SampleT2755.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146836 | GPL1261 |
|
Control HSC, biological rep2
|
Control LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from control HSCs
|
| Sample_geo_accession | GSM1146836
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146836/suppl/GSM1146836_PE022008_SampleT2758.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146836/suppl/GSM1146836_PE022008_SampleT2758.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146837 | GPL1261 |
|
Mll deficient HSC, biological rep3
|
Mll deficient LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mx1-cre;Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from Mll deficient HSCs
|
| Sample_geo_accession | GSM1146837
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146837/suppl/GSM1146837_PE022008_SampleT2759.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146837/suppl/GSM1146837_PE022008_SampleT2759.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146838 | GPL1261 |
|
Mll deficient HSC, biological rep4
|
Mll deficient LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mx1-cre;Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from Mll deficient HSCs
|
| Sample_geo_accession | GSM1146838
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146838/suppl/GSM1146838_PE022008_SampleT2766.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146838/suppl/GSM1146838_PE022008_SampleT2766.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146839 | GPL1261 |
|
Control HSC, biological rep3
|
Control LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from control HSCs
|
| Sample_geo_accession | GSM1146839
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146839/suppl/GSM1146839_PE022008_SampleT2767.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146839/suppl/GSM1146839_PE022008_SampleT2767.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146840 | GPL1261 |
|
Control HSC, biological rep4
|
Control LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from control HSCs
|
| Sample_geo_accession | GSM1146840
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146840/suppl/GSM1146840_PE022008_SampleT2768.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146840/suppl/GSM1146840_PE022008_SampleT2768.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
| | |
|
GSM1146841 | GPL1261 |
|
Mll deficient HSC, biological rep5
|
Mll deficient LSK/CD48-
|
tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mx1-cre;Mll F/F
cell type: hematopoietic stem cells (HSCs)
|
Gene expression data from Mll deficient HSCs
|
| Sample_geo_accession | GSM1146841
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146841/suppl/GSM1146841_PE022008_SampleT2769.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146841/suppl/GSM1146841_PE022008_SampleT2769.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
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GSM1146842 | GPL1261 |
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Control HSC, biological rep5
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Control LSK/CD48-
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tissue: LSK/CD48- HSCs from adult bone marrow
genotype: Mll F/F
cell type: hematopoietic stem cells (HSCs)
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Gene expression data from control HSCs
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| Sample_geo_accession | GSM1146842
| | Sample_status | Public on May 24 2013
| | Sample_submission_date | May 23 2013
| | Sample_last_update_date | May 24 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Injections of pI:pC (10ug per gram mouse + 50ug) were administered every 48 hours for 6 days (3 injections total) to 5 control Mll F/F mice or 5 Mx1-cre; Mll F/F mice to induce deletion. On day 6, bone marrow was harvested and LSK/CD48- cells were sorted to enrich for hematopoietic stem cells.
| | Sample_growth_protocol_ch1 | 8 wk old females with a B6.SJL-Ptprca Pep3b/BoyJ genetic background.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted with TRIzol (Invitrogen) and cleaned with RNeasy columns (Qiagen). Total RNA was amplified with a MessageAMP II aRNA Amplification Kit (Ambion) prior to labeling.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Amplified RNA was labeled with Biotin using the BioArray HighYield RNA Transcript Labeling Kit (Enzo Life Sciences)
| | Sample_hyb_protocol | Fragmentation and hybridization to GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_scan_protocol | Scanning of the GeneChip Mouse Genome 430 2.0 chips (Affymextrix) was carried out by the Dartmouth Medical School Genomics Shared Resource following standard protocols.
| | Sample_data_processing | Data was analyzed with Microarray Suite Version 5.0 software (Affymetrix).
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Erika,Louiez,Artinger
| | Sample_contact_email | erika.l.artinger@dartmouth.edu
| | Sample_contact_laboratory | Patricia A. Ernst Laboratory
| | Sample_contact_department | Genetics
| | Sample_contact_institute | Dartmouth Medical School
| | Sample_contact_address | 725 Remsen
| | Sample_contact_city | Hanover
| | Sample_contact_state | New Hampshire
| | Sample_contact_zip/postal_code | 03755
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146842/suppl/GSM1146842_PE022008_SampleT2770.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1146nnn/GSM1146842/suppl/GSM1146842_PE022008_SampleT2770.CHP.gz
| | Sample_series_id | GSE47205
| | Sample_data_row_count | 45101
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