Search results for the GEO ID: GSE47436  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1149498 | GPL570 |
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SAECs (PD = 18) treated with control siRNA for 72 h rep1
|
SAECs treated with control siRNA for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: control siRNA for 72 h
|
Gene expression data from SAECs treated with control siRNA for 72 h
|
| Sample_geo_accession | GSM1149498
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149498/suppl/GSM1149498_SAEC_si-control-1.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
| | |
|
GSM1149499 | GPL570 |
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SAECs (PD = 18) treated with control siRNA for 72 h rep2
|
SAECs treated with control siRNA for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: control siRNA for 72 h
|
Gene expression data from SAECs treated with control siRNA for 72 h
|
| Sample_geo_accession | GSM1149499
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149499/suppl/GSM1149499_SAEC_si-control-2.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
| | |
|
GSM1149500 | GPL570 |
|
SAECs (PD = 18) treated with si-p53-#1 for 72 h rep1
|
SAECs treated with si-p53-#1 for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: treated with si-p53-#1 for 72 h
|
Gene expression data from SAECs treated with si-p53-#1 for 72 h
|
| Sample_geo_accession | GSM1149500
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149500/suppl/GSM1149500_SAEC_si-p53-_1-1.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
| | |
|
GSM1149501 | GPL570 |
|
SAECs (PD = 18) treated with si-p53-#1 for 72 h rep2
|
SAECs treated with si-p53-#1 for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: treated with si-p53-#1 for 72 h
|
Gene expression data from SAECs treated with si-p53-#1 for 72 h
|
| Sample_geo_accession | GSM1149501
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149501/suppl/GSM1149501_SAEC_si-p53-_1-2.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
| | |
|
GSM1149502 | GPL570 |
|
SAECs (PD = 18) treated with si-p53-#2 for 72 h rep1
|
SAECs treated with si-p53-#2 for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: treated with si-p53-#2 for 72 h
|
Gene expression data from SAECs treated with si-p53-#2 for 72 h
|
| Sample_geo_accession | GSM1149502
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149502/suppl/GSM1149502_SAEC_si-p53-_2-1.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
| | |
|
GSM1149503 | GPL570 |
|
SAECs (PD = 18) treated with si-p53-#2 for 72 h rep2
|
SAECs treated with si-p53-#2 for 72 h
|
tissue: Human lung small airway epithelia
gender: Male
age: 25
treatment: treated with si-p53-#2 for 72 h
|
Gene expression data from SAECs treated with si-p53-#2 for 72 h
|
| Sample_geo_accession | GSM1149503
| | Sample_status | Public on May 29 2013
| | Sample_submission_date | May 28 2013
| | Sample_last_update_date | May 29 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 = SAECs (PD | 18) were transfected with the siRNAs (si-control, si-p53-#1 or si-p53-#2) using Lipofectamine 2000 (Invitrogen), and 24 h after transfection, the medium was replaced. The transfected cells were further incubated for 48 h.
| | Sample_growth_protocol_ch1 | SAECs were cultured at 37 °C in small airway epithelial basal medium supplemented with 52 μg/ml bovine pituitary extract, 0.5 ng/ml human recombinant epidermal growth factor, 0.5 μg/ml epinephrine, 1 μg/ml hydrocortisone, 10 μg/ml transferrin, 5 μg/ml insulin, 0.1 ng/ml retinoic acid , 6.5 ng/ml triiodothyronine, 50 μg/ml Gentamicin/Amphotericin-B, and 50 μg/ml fatty acid-free bovine serum albumin, according to the manufacturer's instructions (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was purified using an RNeasy kit (Qiagen), according to the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Target cRNA was prepared from 3 μg of total RNA with a One-cycle cDNA Synthesis Kit and 3’-amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | Arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G.
| | Sample_data_processing | The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Masato,,Enari
| | Sample_contact_email | menari@ncc.go.jp
| | Sample_contact_institute | National Cancer Center
| | Sample_contact_address | 5-1-1 Tsukiji
| | Sample_contact_city | Tokyo
| | Sample_contact_zip/postal_code | 104-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1149nnn/GSM1149503/suppl/GSM1149503_SAEC_si-p53-_2-2.CEL.gz
| | Sample_series_id | GSE47436
| | Sample_data_row_count | 54613
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