Search results for the GEO ID: GSE47920  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1162416 | GPL570 |
|
T-iPS-derived T lymphocytes experimental replicate 1
|
T-iPS-derived T lymphocytes
|
cell type: T-iPS-derived T cells at day 33 of differentiation
|
Gene expression data from T-iPS-derived T lymphocytes
|
| Sample_geo_accession | GSM1162416
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162416/suppl/GSM1162416_D25_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162417 | GPL570 |
|
T-iPS-derived T lymphocytes experimental replicate 2
|
T-iPS-derived T lymphocytes
|
cell type: T-iPS-derived T cells at day 35 of differentiation
|
Gene expression data from T-iPS-derived T lymphocytes
|
| Sample_geo_accession | GSM1162417
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162417/suppl/GSM1162417_D29_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162418 | GPL570 |
|
T-iPS-derived T lymphocytes experimental replicate 3
|
T-iPS-derived T lymphocytes
|
cell type: T-iPS-derived T cells at day 30 of differentiation
|
Gene expression data from T-iPS-derived T lymphocytes
|
| Sample_geo_accession | GSM1162418
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162418/suppl/GSM1162418_D32_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162419 | GPL570 |
|
CD4 T cells biological replicate 1
|
CD4 T cells
|
cell type: CD3+CD4+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD4+ T lymphocytes
|
| Sample_geo_accession | GSM1162419
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162419/suppl/GSM1162419_CD4_1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162420 | GPL570 |
|
CD4 T cells biological replicate 2
|
CD4 T cells
|
cell type: CD3+CD4+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD4+ T lymphocytes
|
| Sample_geo_accession | GSM1162420
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162420/suppl/GSM1162420_CD4_2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162421 | GPL570 |
|
CD8 T cells biological replicate 1
|
CD8 T cells
|
cell type: CD3+CD8+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD8+ T lymphocytes
|
| Sample_geo_accession | GSM1162421
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162421/suppl/GSM1162421_CD8_1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162422 | GPL570 |
|
CD8 T cells biological replicate 2
|
CD8 T cells
|
cell type: CD3+CD8+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD8+ T lymphocytes
|
| Sample_geo_accession | GSM1162422
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162422/suppl/GSM1162422_CD8_2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162423 | GPL570 |
|
CD3+CD56+ T cells biological replicate 1
|
CD3+CD56+ T cells
|
cell type: CD3+CD56+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD56+ T lymphocytes
|
| Sample_geo_accession | GSM1162423
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162423/suppl/GSM1162423_CD3CD56_1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162424 | GPL570 |
|
CD3+CD56+ T cells biological replicate 2
|
CD3+CD56+ T cells
|
cell type: CD3+CD56+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+CD56+ T lymphocytes
|
| Sample_geo_accession | GSM1162424
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162424/suppl/GSM1162424_CD3CD56_2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162425 | GPL570 |
|
γδ Τ cells biological replicate 1
|
γδ Τ cells
|
cell type: CD3+TCRγδ+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+TCRγδ+ T lymphocytes
|
| Sample_geo_accession | GSM1162425
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162425/suppl/GSM1162425_GD_1_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
| | |
|
GSM1162426 | GPL570 |
|
γδ Τ cells biological replicate 2
|
γδ Τ cells
|
cell type: CD3+TCRγδ+ sorted T cells from peripheral blood
|
Gene expression data from sorted peripheral blood CD3+TCRγδ+ T lymphocytes
|
| Sample_geo_accession | GSM1162426
| | Sample_status | Public on Aug 06 2013
| | Sample_submission_date | Jun 13 2013
| | Sample_last_update_date | Aug 06 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_growth_protocol_ch1 | T-iPS cells were differentiated to hematopoietic progenitors through embryoid body formation and next to T lymphocytes by co-culture with OP9-DL1 feeder cells. T-iPS-derived T lymphocytes were isolated at day 30-35 of differentiation (20-25 OP9-DL1 co-culture). Fresh peripheral blood lymphocytes were isolated from two healthy donors after informed consent with Ficoll density centrifugation. The following subpopulations CD3+CD4+, CD3+CD8+, CD3+CD56+, and CD3+TCRγδ+ (γδ Τ cells) were purified (98%) by cell sorting.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total mRNA was extracted from T-iPS-T cells at day 30-35 of differentiation and from the sorted PBL subpopulations using TRIzolTM Reagent
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 75ng total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix 3000 7G Scanner.
| | Sample_data_processing | The data were processed with Affymetrix GeneChip Command Console using Affymetrix default settings.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Michel,,Sadelain
| | Sample_contact_email | m-sadelain@ski.mskcc.org
| | Sample_contact_institute | Memorial Sloan-Kettering Cancer Center
| | Sample_contact_address | 1250 1st Ave
| | Sample_contact_city | New York
| | Sample_contact_zip/postal_code | 10065
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1162nnn/GSM1162426/suppl/GSM1162426_GD_2_HG-U133_Plus_2.CEL.gz
| | Sample_series_id | GSE47920
| | Sample_data_row_count | 54675
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