Search results for the GEO ID: GSE48258  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1173423 | GPL570 |
|
Untreated CLL1
|
Primary CLL cells (untreated)
|
treatment: untreated
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173423
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173423/suppl/GSM1173423_Untreated_CLL1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173423/suppl/GSM1173423_Untreated_CLL1.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173424 | GPL570 |
|
Untreated CLL2
|
Primary CLL cells (untreated)
|
treatment: untreated
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173424
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173424/suppl/GSM1173424_Untreated_CLL2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173424/suppl/GSM1173424_Untreated_CLL2.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173425 | GPL570 |
|
Untreated CLL3
|
Primary CLL cells (untreated)
|
treatment: untreated
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173425
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173425/suppl/GSM1173425_Untreated_CLL3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173425/suppl/GSM1173425_Untreated_CLL3.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173426 | GPL570 |
|
CDKI-73 CLL1
|
Primary CLL cells (treated with 0.1 μM CDKI-73 for 4h)
|
treatment: 0.1 μM CDKI-73 for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173426
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173426/suppl/GSM1173426_CDKI-73_CLL1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173426/suppl/GSM1173426_CDKI-73_CLL1.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173427 | GPL570 |
|
CDKI-73 CLL2
|
Primary CLL cells (treated with 0.1 μM CDKI-73 for 4h)
|
treatment: 0.1 μM CDKI-73 for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173427
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173427/suppl/GSM1173427_CDKI-73_CLL2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173427/suppl/GSM1173427_CDKI-73_CLL2.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173428 | GPL570 |
|
CDKI-73 CLL3
|
Primary CLL cells (treated with 0.1 μM CDKI-73 for 4h)
|
treatment: 0.1 μM CDKI-73 for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173428
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173428/suppl/GSM1173428_CDKI-73_CLL3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173428/suppl/GSM1173428_CDKI-73_CLL3.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173429 | GPL570 |
|
Fludara CLL1
|
Primary CLL cells (treated with 10 μM fludarabine for 4h)
|
treatment: 10 μM fludarabine for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173429
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173429/suppl/GSM1173429_Fludara_CLL1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173429/suppl/GSM1173429_Fludara_CLL1.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173430 | GPL570 |
|
Fludara CLL2
|
Primary CLL cells (treated with 10 μM fludarabine for 4h)
|
treatment: 10 μM fludarabine for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173430
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173430/suppl/GSM1173430_Fludara_CLL2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173430/suppl/GSM1173430_Fludara_CLL2.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173431 | GPL570 |
|
Fludara CLL3
|
Primary CLL cells (treated with 10 μM fludarabine for 4h)
|
treatment: 10 μM fludarabine for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173431
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173431/suppl/GSM1173431_Fludara_CLL3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173431/suppl/GSM1173431_Fludara_CLL3.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173432 | GPL570 |
|
Combination CLL1
|
Primary CLL cells (treated with 0.1uM CDKI-73 and 10 μM fludarabine for 4h)
|
treatment: 0.1uM CDKI-73 and 10 μM fludarabine for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173432
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173432/suppl/GSM1173432_Combination_CLL1.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173432/suppl/GSM1173432_Combination_CLL1.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173433 | GPL570 |
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Combination CLL2
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Primary CLL cells (treated with 0.1uM CDKI-73 and 10 μM fludarabine for 4h)
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treatment: 0.1uM CDKI-73 and 10 μM fludarabine for 4h
cell type: CLL B-cells
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| Sample_geo_accession | GSM1173433
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173433/suppl/GSM1173433_Combination_CLL2.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173433/suppl/GSM1173433_Combination_CLL2.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
| | |
|
GSM1173434 | GPL570 |
|
Combination CLL3
|
Primary CLL cells (treated with 0.1uM CDKI-73 and 10 μM fludarabine for 4h)
|
treatment: 0.1uM CDKI-73 and 10 μM fludarabine for 4h
cell type: CLL B-cells
|
|
| Sample_geo_accession | GSM1173434
| | Sample_status | Public on Jun 25 2013
| | Sample_submission_date | Jun 24 2013
| | Sample_last_update_date | Jun 25 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 5x106 CLL cells were treated with 0.1 μM CDKI-73, 10 μM fludarabine or the two drugs in combination for 4h.
| | Sample_growth_protocol_ch1 | Primary CLL cells were cultured in RPMI supplemented with 10% fetal bovine serum (FBS), penicillin (50 U/ml), streptomycin (50 µg/ml) and recombinant human IL-4 (R and D Systems, Abingdon, UK) (5 ng/ml).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA.
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix U133 2.0 Gene Chips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with MADRAS bespoke software using Affymetrix default analysis settings and global scaling as normalization method.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Chris,,Pepper
| | Sample_contact_email | peppercj@cf.ac.uk
| | Sample_contact_phone | +44 2920687057
| | Sample_contact_institute | Cardiff University
| | Sample_contact_address | Heath Park
| | Sample_contact_city | Cardiff
| | Sample_contact_zip/postal_code | CF14 4XN
| | Sample_contact_country | United Kingdom
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173434/suppl/GSM1173434_Combination_CLL3.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1173nnn/GSM1173434/suppl/GSM1173434_Combination_CLL3.CHP.gz
| | Sample_series_id | GSE48258
| | Sample_data_row_count | 54675
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