Result

Search results for the GEO ID: GSE48567

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Title

Source name

Description

Characteristics

GSM1181236

GPL570

A224_FGF18-overexpression_rep1

A224 cells with FGF18 overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing human FGF18 cDNA

Gene expression data from FGF18 overexpressing A224 cells A224 FGF18 1

Sample_geo_accession	GSM1181236
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181236/suppl/GSM1181236_A224_FGF18_1_1685A.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

GSM1181237

GPL570

A224_FGF18-overexpression_rep2

A224 cells with FGF18 overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing human FGF18 cDNA

Gene expression data from FGF18 overexpressing A224 cells A224 FGF18 2

Sample_geo_accession	GSM1181237
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181237/suppl/GSM1181237_A224_FGF18_2_1685B.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

GSM1181238

GPL570

A224_FGF18-overexpression_rep3

A224 cells with FGF18 overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing human FGF18 cDNA

Gene expression data from FGF18 overexpressing A224 cells A224 FGF18 3

Sample_geo_accession	GSM1181238
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181238/suppl/GSM1181238_A224_FGF18_3_1685C.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

GSM1181239

GPL570

A224_RFP-overexpression_rep1

A224 cells with RFP overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing RFP cDNA

Gene expression data from RFP overexpressing A224 cells A224 RFP 1

Sample_geo_accession	GSM1181239
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181239/suppl/GSM1181239_A224_RFP_1_1685D.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

GSM1181240

GPL570

A224_RFP-overexpression_rep2

A224 cells with RFP overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing RFP cDNA

Gene expression data from RFP overexpressing A224 cells A224 RFP 2

Sample_geo_accession	GSM1181240
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181240/suppl/GSM1181240_A224_RFP_2_1685E.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

GSM1181241

GPL570

A224_RFP-overexpression_rep3

A224 cells with RFP overexpression

cell line: A224 human epithelial ovarian cancer cell line genotype/variation: stably infected with lentivirus expressing RFP cDNA

Gene expression data from RFP overexpressing A224 cells A224 RFP 3

Sample_geo_accession	GSM1181241
Sample_status	Public on Jul 08 2013
Sample_submission_date	Jul 06 2013
Sample_last_update_date	Jul 08 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	Ectopic overexpression was performed by lentiviral vector mediated delivery of FGF18 cDNA or RFP cDNA (pLoc series, Thermo Scientific). Stably infected cells were maintained in medium containing blasticidin S (15 μg/ml).
Sample_growth_protocol_ch1	A224 cells were grown in RPMI1640 supplemented with 10% FBS and penicillin/streptomycin (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA samples were extracted from cells at ~70-80% confluence using RNeasy Mini Kit and RNase-Free DNase Set (both from QIAGEN) for on-column gDNA removal during RNA purification.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	One-round amplification were completed according to standard Affymetrix One-Cycle Amplification protocol using 2.5μg of total RNA.
Sample_hyb_protocol	A 15 μg aliquot of amplified biotinylated cRNA was hybridized to a Human U133 Plus 2.0 GeneChip array (Affymetrix, Santa Clara, CA).
Sample_scan_protocol	Arrays were scanned using the laser confocal GeneChip Scanner 3000 (Affymetrix).
Sample_data_processing	JustRMA algorithm and median intensity of entire array were applied for data input and normalization by BRB ArrayTools version 4.1.0 (developed by Dr. Richard Simon et al, NCI, NIH). Differentially regulated genes were identified by a univariate paired t-test (p<0.0001, fold-change>2.0, random-variance model) from probe sets presenting in all arrays and displaying a variance in the top 50th percentile.
Sample_platform_id	GPL570
Sample_contact_name	Michael,,Birrer
Sample_contact_email	mbirrer@partners.org
Sample_contact_phone	6177244800
Sample_contact_laboratory	Surgical Oncology Research Labs
Sample_contact_department	Medicine
Sample_contact_institute	MGH
Sample_contact_address	70 Blossom Street
Sample_contact_city	Boston
Sample_contact_state	MA
Sample_contact_zip/postal_code	02114
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1181nnn/GSM1181241/suppl/GSM1181241_A224_RFP_3_1685F.CEL.gz
Sample_series_id	GSE48567
Sample_data_row_count	54670

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