Result

Search results for the GEO ID: GSE4883

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Title

Source name

Description

Characteristics

GSM109787

GPL570

Control 1, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109787
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	Untreated control sample
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109787/suppl/GSM109787.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109788

GPL570

Control 2, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109788
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	Untreated control sample
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109788/suppl/GSM109788.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109789

GPL570

Control 3, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109789
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	Untreated control sample
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109789/suppl/GSM109789.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109790

GPL570

Simvastatin treated 12h, sample 1, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109790
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	12h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109790/suppl/GSM109790.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109791

GPL570

Simvastatin treated 12h, sample 2, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109791
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	12h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109791/suppl/GSM109791.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109792

GPL570

Simvastatin treated 12h, sample 3, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109792
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	12h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109792/suppl/GSM109792.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109793

GPL570

Simvastatin treated 24h, sample 1, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109793
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	24h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109793/suppl/GSM109793.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109794

GPL570

Simvastatin treated 24h, sample 2, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109794
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	24h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109794/suppl/GSM109794.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

GSM109795

GPL570

Simvastatin treated 24h, sample 3, Simvastatin is anti-inflammatory in macrophages

human peripheral blood monocyte-derived macrophages

from unknown healthy blood-donor volunteers (Finnish Red Cross)

Sample_geo_accession	GSM109795
Sample_status	Public on Dec 31 2006
Sample_submission_date	May 22 2006
Sample_last_update_date	May 24 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_biomaterial_provider_ch1	Finnish Red Cross
Sample_treatment_protocol_ch1	24h before sample collection cells were stimulated with 10 um simvastatin in serum free medium.
Sample_growth_protocol_ch1	HPBM Cells were isolated from buffy coats using Ficoll-Paque gradient centrifugation (as described Pietarinen-Runtti, et al. Am.J Physiol Cell Physiol. 2000;278:C118-125). During isolation, monocytes from 3 individuals were pooled. Adherent cells were cultured in standard medium (20% human serum, Cambrex for differentiation into macrophages. The macrophage-phenotype at day 7 after isolation was confirmed with the typical shape of macrophages and also by macrophage-immunostaining (mAB CD68, DAKO, Denmark, dilution 1:200), where macrophages presented >95% of the cell population. 36 hours prior to sample collection cell growth medium was changed to serum free medium.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from the cells using Trizol reagent (Gibco BRL, USA) according to manufacturers’ instructions. The quality and amount of RNA was assessed by spectrophotometry (NanoDrop, USA) and by agarose gel electrophoresis.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	cDNAs were prepared from RNAs (5 ug of RNA) with reverse transcriptase (Superscript II primed by a poly (T) oligomer/T7 promoter). cDNAs were subsequently used as a template to make biotin-labeled cRNA with an in vitro transcription reaction according to Affymetrix standard protocol.
Sample_hyb_protocol	cRNAs (15 ug) were hybridized to Affymetrix HGU133 Plus 2.0 oligonucleotide arrays, which was processed and scanned according to manufacturer’s instructions.
Sample_scan_protocol	Standard Affymetrix protocoll.
Sample_data_processing	Affymetrix GeneChip® Operating Software (GCOS) analysis
Sample_platform_id	GPL570
Sample_contact_name	Tiina,,Tuomisto
Sample_contact_email	tiina.tuomisto@uku.fi
Sample_contact_laboratory	SYH
Sample_contact_department	Molecular Medicine
Sample_contact_institute	A.I.Virtanen Institute
Sample_contact_address	Neulaniementie 2
Sample_contact_city	Kuopio
Sample_contact_zip/postal_code	70210
Sample_contact_country	Finland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109795/suppl/GSM109795.CEL.gz
Sample_series_id	GSE4883
Sample_data_row_count	54675

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