Search results for the GEO ID: GSE4885  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM109801 | GPL96 |
|
Hsrc1
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109801
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | untreated control cells
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109801/suppl/GSM109801.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109802 | GPL96 |
|
Hsrc2
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109802
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | untreated control cells
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109802/suppl/GSM109802.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109803 | GPL96 |
|
Hsrc3
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line treated with interleukin-6 for 1 hour
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109803
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 1 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109803/suppl/GSM109803.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109804 | GPL96 |
|
Hsrc4
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line
treated with interleukin-6 for 1 hour
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109804
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 1 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109804/suppl/GSM109804.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109805 | GPL96 |
|
Hsrc5
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line
treated with interleukin-6 for 4 hours
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109805
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 4 hours
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109805/suppl/GSM109805.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109806 | GPL96 |
|
Hsrc6
|
HepG2
|
HepG2 human hepatocellular carcinoma cell line
treated with interleukin-6 for 4 hours
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109806
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 4 hours
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109806/suppl/GSM109806.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109807 | GPL96 |
|
Hsrc7
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1).
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109807
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Untreated control cells.
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109807/suppl/GSM109807.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109808 | GPL96 |
|
Hsrc8
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1).
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109808
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Untreated control cells.
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109808/suppl/GSM109808.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109809 | GPL96 |
|
Hsrc9
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1). Cells were treated with interleukin-6 for 1 h.
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109809
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 1 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109809/suppl/GSM109809.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109810 | GPL96 |
|
Hsrc10
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1). Cells were treated with interleukin-6 for 1 h.
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109810
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 1 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109810/suppl/GSM109810.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109811 | GPL96 |
|
Hsrc11
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1). Cells were treated with interleukin-6 for 4 h.
|
This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
|
| Sample_geo_accession | GSM109811
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 4 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109811/suppl/GSM109811.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
| | |
|
GSM109812 | GPL96 |
|
Hsrc12
|
HepG2-∆Src1
|
HepG2-∆Src1 cells were generated by lentiviral infection of the HepG2 human hepatocellular carcinoma cell line with a vector coding for a small-hairpin RNA that knocks down expression of the coactivator SRC-1/NcoA-1 (nuclear receptor coactivator 1). Cells were treated with interleukin-6 for 4 h.
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This sample is part of a experiment series addressing the role of coactivator SRC-1/NcoA-1 for the induction of interleukin-6 (IL-6) target genes in HepG2 cells. Either control HepG2 cells or HepG2 cells manipulated to stably express an shRNA that knocks down SRC-1 expression were treated with IL-6 for 1 or 4 h or left untreated.
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| Sample_geo_accession | GSM109812
| | Sample_status | Public on Dec 31 2006
| | Sample_submission_date | May 22 2006
| | Sample_last_update_date | May 22 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells treated with 10 ng/ml interleukin-6 for 4 hour
| | Sample_growth_protocol_ch1 | HepG2 cells were cultured in a 2:1 (v/v) mixture of Dulbecco's minimal essential medium (DMEM) and Ham's F12 medium with 10% (v/v) fetal calf serum and 1% (w/v) antibiotics (penicillin and streptomycin), at 37 degrees C and 5 % CO2.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was prepared using the Qiagen RNeasy Mini Kit according to the manufacturer’s instructions. RNA quality was examined by the RNA 6000 LabChip Kit on the Agilent 2100 bioanalyzer.
| | Sample_label_ch1 | Phycoerythrin
| | Sample_data_processing | Affymetrix MAS5.0
| | Sample_platform_id | GPL96
| | Sample_contact_name | Friedemann,,Horn
| | Sample_contact_email | fhorn@medizin.uni-leipzig.de
| | Sample_contact_laboratory | Molecular Immunology
| | Sample_contact_department | Institute of Clinical Immunology and Transfusion Medicine
| | Sample_contact_institute | University of Leipzig
| | Sample_contact_address | Johannisalle 30
| | Sample_contact_city | Leipzig
| | Sample_contact_zip/postal_code | 04103
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM109nnn/GSM109812/suppl/GSM109812.CEL.gz
| | Sample_series_id | GSE4885
| | Sample_data_row_count | 22283
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