Result

Search results for the GEO ID: GSE49117

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Title

Source name

Description

Characteristics

GSM1193981

GPL1261

DI_32Dcl3_ASXL1_IL-3

32Dcl3 cells transduced with ASXL1-MT, treated with IL-3

cell line: 32Dcl3 transfection: pMYs-FLAG-ASXL1-MT-IG treatment: treated with IL-3

Sample_geo_accession	GSM1193981
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193981/suppl/GSM1193981_DI_32Dcl3_ASXL1_IL3.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193982

GPL1261

DI_32Dcl3_ASXL1_G2h

32Dcl3 cells transduced with ASXL1-MT, treated with G-CSF for 2 hours

cell line: 32Dcl3 transfection: pMYs-FLAG-ASXL1-MT-IG treatment: treated with G-CSF for 2 hours

Sample_geo_accession	GSM1193982
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193982/suppl/GSM1193982_DI_32Dcl3_ASXL1_G2h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193983

GPL1261

DI_32Dcl3_ASXL1_G6h

32Dcl3 cells transduced with ASXL1-MT, treated with G-CSF for 6 hours

cell line: 32Dcl3 transfection: pMYs-FLAG-ASXL1-MT-IG treatment: treated with G-CSF for 6 hours

Sample_geo_accession	GSM1193983
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193983/suppl/GSM1193983_DI_32Dcl3_ASXL1_G6h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193984

GPL1261

DI_32Dcl3_ASXL1_G24h

32Dcl3 cells transduced with ASXL1-MT, treated with G-CSF for 24 hours

cell line: 32Dcl3 transfection: pMYs-FLAG-ASXL1-MT-IG treatment: treated with G-CSF for 24 hours

Sample_geo_accession	GSM1193984
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193984/suppl/GSM1193984_DI_32Dcl3_ASXL1_G24h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193985

GPL1261

DI_32Dcl3_mock_IL-3

32Dcl3 cells transduced with mock, treated with IL-3

cell line: 32Dcl3 transfection: pMYs-IG (mock) treatment: treated with IL-3

Sample_geo_accession	GSM1193985
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193985/suppl/GSM1193985_DI_32Dcl3_mock_IL3.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193986

GPL1261

DI_32Dcl3_mock_G2h

32Dcl3 cells transduced with mock, treated with G-CSF for 2 hours

cell line: 32Dcl3 transfection: pMYs-IG (mock) treatment: treated with G-CSF for 2 hours

Sample_geo_accession	GSM1193986
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193986/suppl/GSM1193986_DI_32Dcl3_mock_G2h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193987

GPL1261

DI_32Dcl3_mock_G6h

32Dcl3 cells transduced with mock, treated with G-CSF for 6 hours

cell line: 32Dcl3 transfection: pMYs-IG (mock) treatment: treated with G-CSF for 6 hours

Sample_geo_accession	GSM1193987
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193987/suppl/GSM1193987_DI_32Dcl3_mock_G6h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

GSM1193988

GPL1261

DI_32Dcl3_mock_G24h

32Dcl3 cells transduced with mock, treated with G-CSF for 24 hours

cell line: 32Dcl3 transfection: pMYs-IG (mock) treatment: treated with G-CSF for 24 hours

Sample_geo_accession	GSM1193988
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 23 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
Sample_hyb_protocol	10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
Sample_scan_protocol	Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
Sample_data_processing	Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using RMA algorithm with target intensity=100.
Sample_platform_id	GPL1261
Sample_contact_name	Daichi,,Inoue
Sample_contact_email	daichi-i@hotmail.co.jp
Sample_contact_institute	Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
Sample_contact_address	4-6-1 Shirokanedai, Minato-ku
Sample_contact_city	Tokyo
Sample_contact_zip/postal_code	108-8639
Sample_contact_country	Japan
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193988/suppl/GSM1193988_DI_32Dcl3_mock_G24h.CEL.gz
Sample_series_id	GSE49117
Sample_data_row_count	45037

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