Search results for the GEO ID: GSE49118 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1193989 | GPL1261 |
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BMT-ASXL1Mt-1
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Myeloid BM cells derived from ASXL1-MT-transplanted mice (myelodysplastic symdrome-like disease)
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cell type: myeloid BM cells
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Sample_geo_accession | GSM1193989
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193989/suppl/GSM1193989_BMT_ASXL1Mt_1.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
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GSM1193990 | GPL1261 |
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BMT-ASXL1Mt-2
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Myeloid BM cells derived from ASXL1-MT-transplanted mice (myelodysplastic symdrome-like disease)
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cell type: myeloid BM cells
|
|
Sample_geo_accession | GSM1193990
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193990/suppl/GSM1193990_BMT_ASXL1Mt_2.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
|
GSM1193991 | GPL1261 |
|
BMT-ASXL1Mt-3
|
Myeloid BM cells derived from ASXL1-MT-transplanted mice (myelodysplastic symdrome-like disease)
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cell type: myeloid BM cells
|
|
Sample_geo_accession | GSM1193991
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193991/suppl/GSM1193991_BMT_ASXL1Mt_3.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
|
GSM1193992 | GPL1261 |
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BMT-mock-1
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CD3-B220-Ter119- Myeloid BM cells derived from mock-transplanted mice (normal control)
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cell type: myeloid BM cells
|
|
Sample_geo_accession | GSM1193992
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193992/suppl/GSM1193992_BMT_mock_1.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
|
GSM1193993 | GPL1261 |
|
BMT-mock-2
|
CD3-B220-Ter119- Myeloid BM cells derived from mock-transplanted mice (normal control)
|
cell type: myeloid BM cells
|
|
Sample_geo_accession | GSM1193993
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193993/suppl/GSM1193993_BMT_mock_2_430.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
|
GSM1193994 | GPL1261 |
|
BMT-mock-3
|
CD3-B220-Ter119- Myeloid BM cells derived from mock-transplanted mice (normal control)
|
cell type: myeloid BM cells
|
|
Sample_geo_accession | GSM1193994
| Sample_status | Public on Jul 31 2013
| Sample_submission_date | Jul 23 2013
| Sample_last_update_date | Jul 31 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNAs were extracted using TRIzol (Invitrogen, Carlsbad, CA). 3 micrograms of total RNA was reverse transcribed using the SuperScript III system for cDNA synthesis (Invitrogen) according to the protocol recommended by Affymetrix. The oligonucleotide used for priming was 5'-ggccagtgaattgtaatacgactcactatagggaggcgg-(t)24-3' (Invitrogen). Double-stranded cDNA was cleaned by phenol:chloroform extraction and the aqueous phase removed by centrifugation through Phase-lock Gel (Eppendorf).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | In vitro transcription was performed on 1 microgram of cDNA using the Enzo BioArray High Yield RNA transcript labelling kit (Enzo Diagnostics). The cRNA was cleaned using RNAeasy clean-up columns (Qiagen). The cRNA was fragmented by heating in 40 mM Tris-acetate pH 8.1, 100 mM KOAc, 30 mM MgOAc.
| Sample_hyb_protocol | 10 micrograms of fragmented cRNA were hybridised (45degree CelsiusC, 16 hours). Hybridization was controled by use of the GeneChip Eukaryotic Hybridization Control Kit (Affymetrix). Washing and staining was performed in a Fluidics Station 450 (Affymetrix) using the protocol EukGE-WS2v4.
| Sample_scan_protocol | Scanning was performed in an Affymetrix GeneChip scanner 3000 7G.
| Sample_data_processing | Genechip analysis was performed using the Affymetrix GeneChip Operating Software v1.3. Signal value was calculated using MAS5 algorithm.
| Sample_platform_id | GPL1261
| Sample_contact_name | Daichi,,Inoue
| Sample_contact_email | daichi-i@hotmail.co.jp
| Sample_contact_institute | Division of Cellular Therapy, Advanced Clinical Research Center, The Institute of Medical Science, The University of Tokyo
| Sample_contact_address | 4-6-1 Shirokanedai, Minato-ku
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 108-8639
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1193nnn/GSM1193994/suppl/GSM1193994_BMT_mock_3_430.CEL.gz
| Sample_series_id | GSE49118
| Sample_data_row_count | 45037
| |
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