Search results for the GEO ID: GSE49283  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM1196651 | GPL1261 |
|
1 dpp Non-translating, biological rep1
|
Mouse 1 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196651
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196651/suppl/GSM1196651_D1_Non_1_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196652 | GPL1261 |
|
1 dpp Non-translating, biological rep2
|
Mouse 1 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196652
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196652/suppl/GSM1196652_D1_Non_2_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196653 | GPL1261 |
|
1 dpp Non-translating, biological rep3
|
Mouse 1 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196653
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196653/suppl/GSM1196653_D1_Non_3_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196654 | GPL1261 |
|
1 dpp Polysome translating, biological rep1
|
Mouse 1 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196654
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196654/suppl/GSM1196654_D1_Poly_1_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196655 | GPL1261 |
|
1 dpp Polysome translating, biological rep2
|
Mouse 1 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196655
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196655/suppl/GSM1196655_D1_Poly_2_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196656 | GPL1261 |
|
1 dpp Polysome translating, biological rep3
|
Mouse 1 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 1 day post partium
|
Gene expression data from 1 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196656
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196656/suppl/GSM1196656_D1_Poly_3_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196657 | GPL1261 |
|
4 dpp Non-translating, biological rep1
|
Mouse 4 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196657
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196657/suppl/GSM1196657_D4_Non_1_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196658 | GPL1261 |
|
4 dpp Non-translating, biological rep2
|
Mouse 4 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196658
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196658/suppl/GSM1196658_D4_Non_2_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196659 | GPL1261 |
|
4 dpp Non-translating, biological rep3
|
Mouse 4 dpp testes non-translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes non-translating mRNAs
|
| Sample_geo_accession | GSM1196659
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196659/suppl/GSM1196659_D4_Non_3_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196660 | GPL1261 |
|
4 dpp Polysome translating, biological rep1
|
Mouse 4 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196660
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196660/suppl/GSM1196660_D4_Poly_1_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196661 | GPL1261 |
|
4 dpp Polysome translating, biological rep2
|
Mouse 4 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196661
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196661/suppl/GSM1196661_D4_Poly_2_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
|
GSM1196662 | GPL1261 |
|
4 dpp Polysome translating, biological rep3
|
Mouse 4 dpp testes translating mRNAs
|
tissue: Testis
genotype: CD-1
age: 4 days post partium
|
Gene expression data from 4 dpp mouse testes translating mRNAs
|
| Sample_geo_accession | GSM1196662
| | Sample_status | Public on Jul 27 2013
| | Sample_submission_date | Jul 26 2013
| | Sample_last_update_date | Jul 27 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Testes were harvested and washed with 1X ice-cold phosphate-buffered saline (PBS) containing cycloheximide to freeze polysomes, then immediately snap-frozen in liquid nitrogen, and stored at -80°C. Testes were pulverized under liquid nitrogen and lysed in 1 ml polysome lysis. Lysates were homogenized and nuclei were pelleted by centrifugation. Cytoplasmic extracts were layered onto a linear sucrose density gradient in polysome gradient and then centrifuged at 210,000 x g at 4°C for 2 h without braking. Gradients were then fractionated using an ISCO gradient fractionation system equipped with a UA-6 detector. RNP fractions, 40S, 60S, 80S, light polysomes, and heavy polysomes were monitored by continuous UV absorption profiles at 254 nm. Fourteen successive 750 μl fractions were collected and stored at -80°C.
| | Sample_growth_protocol_ch1 | All animal procedures were performed in accordance with the National Research Council Guide for the Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of East Carolina University. CD-1 mice were used for all analyses. Euthanasia was performed by decapitation, and testes were snap-frozen in liquid nitrogen and stored at -80°C.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNA was isolated from polysome sucrose fractions by TRIzol extraction (Invitrogen) using manufacturer’s instructions
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotin-labeled amplified antisense RNA was synthesized from 5 ug of RNA by the standard Affymetrix 3’ IVT procedure
| | Sample_hyb_protocol | Hybridized to the Affymetrix 430 2.0 mouse expression array at the core facility at the University of North Carolina, Neuroscience Research Center, Chapel Hill, NC.
| | Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus Targeted Genotyping System.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 using Affymetrix default analysis settings.
| | Sample_platform_id | GPL1261
| | Sample_contact_name | Christopher,B,Geyer
| | Sample_contact_email | geyerc@ecu.edu
| | Sample_contact_phone | 252-744-3433
| | Sample_contact_department | Anatomy and Cell Biology
| | Sample_contact_institute | East Carolina University
| | Sample_contact_address | 600 Moye Boulevard, Mail Stop 620
| | Sample_contact_city | Greenville
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27858
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1196nnn/GSM1196662/suppl/GSM1196662_D4_Poly_3_Mouse430_2.CEL.gz
| | Sample_series_id | GSE49283
| | Sample_data_row_count | 45101
| | |
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