Sample_geo_accession | GSM1197601
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Sample_status | Public on Jul 31 2013
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Sample_submission_date | Jul 30 2013
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Sample_last_update_date | Jul 31 2013
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_growth_protocol_ch1 | MEFs were cultured in Dulbecco’s minimal essential medium containing 10% fetal calf serum and 1% (w/v) penicillin/streptomycin at 37˚C and 5% CO2.
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | Total RNA was isolated from the cells using RNeasy Mini Kit (QIAGEN). The quality of RNA was measured using 2100 Bioanalyzer (Agilent).
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | The total RNA undergoes reverse transcription to synthesize first-strand cDNA. This cDNA is then converted template for transcription into a double-stranded DNA synthesize first-strand cDNA.
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Sample_label_protocol_ch1 | This cDNA is then converted into a double-stranded DNA template for transcription. In vitro transcription synthesizes aRNA and incorporates a biotin-congugated nucleotide.
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Sample_hyb_protocol | Fragmentation of the biotin-labeled aRNA prepares the sample for hybridization onto GeneChip 3’ expression arrays.
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Sample_scan_protocol | GeneChips were scanned using GeneChip Scaner 3000 7G.
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Sample_data_processing | The data were analyzed with Gene Chip operation software (GCOS) Ver. 1.4 using Affymetrix default analysis settings.
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Sample_platform_id | GPL1261
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Sample_contact_name | ayako,,kurimoto
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Sample_contact_institute | RIKEN
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Sample_contact_address | Hirosawa
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Sample_contact_city | Wako-shi
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Sample_contact_state | Saitama
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Sample_contact_zip/postal_code | 351-0198
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Sample_contact_country | Japan
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197601/suppl/GSM1197601_MEF_WT3.CEL.gz
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197601/suppl/GSM1197601_MEF_WT3.CHP.gz
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Sample_series_id | GSE49336
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Sample_data_row_count | 45101
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