Result

Search results for the GEO ID: GSE49351

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM1197826

GPL1261

Transgenic heart with high FASN-rep1

Transgenic heart with high FASN expression

tissue: transgenic heart strain: C57BL/6J phenotype: high FASN gender: male

Gene expression data of heart with high level of transgenic FASN expression

Sample_geo_accession	GSM1197826
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197826/suppl/GSM1197826_FASN-High-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197826/suppl/GSM1197826_FASN-High-1.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

GSM1197827

GPL1261

Transgenic heart with high FASN-rep2

Transgenic heart with high FASN expression

tissue: transgenic heart strain: C57BL/6J phenotype: high FASN gender: male

Gene expression data of heart with high level of transgenic FASN expression

Sample_geo_accession	GSM1197827
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197827/suppl/GSM1197827_FASN-High-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197827/suppl/GSM1197827_FASN-High-2.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

GSM1197828

GPL1261

Transgenic heart with low FASN-rep1

Transgenic heart with low FASN expression

tissue: transgenic heart strain: C57BL/6J phenotype: low FASN gender: male

Gene expression data of heart with low level of transgenic FASN expression

Sample_geo_accession	GSM1197828
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197828/suppl/GSM1197828_FASN-Low-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197828/suppl/GSM1197828_FASN-Low-1.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

GSM1197829

GPL1261

Transgenic heart with low FASN-rep2

Transgenic heart with low FASN expression

tissue: transgenic heart strain: C57BL/6J phenotype: low FASN gender: male

Gene expression data of heart with low level of transgenic FASN expression

Sample_geo_accession	GSM1197829
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197829/suppl/GSM1197829_FASN-Low-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197829/suppl/GSM1197829_FASN-Low-2.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

GSM1197830

GPL1261

B6 control heart rep1

B6 control heart

tissue: transgenic heart strain: C57BL/6J phenotype: control heart gender: male

Gene expression data of B6 control heart

Sample_geo_accession	GSM1197830
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197830/suppl/GSM1197830_B6-1.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197830/suppl/GSM1197830_B6-1.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

GSM1197831

GPL1261

B6 control heart rep2

B6 control heart

tissue: transgenic heart strain: C57BL/6J phenotype: control heart gender: male

Gene expression data of B6 control heart

Sample_geo_accession	GSM1197831
Sample_status	Public on Jul 31 2013
Sample_submission_date	Jul 30 2013
Sample_last_update_date	Jul 31 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	For the microarray study, hearts were isolated from three study groups: (i) Transgenic mice with high cardiac FASN expression, (ii) transgenic mice with low cardiac FASN expression, and (iii) age-matched B6 control mice.
Sample_growth_protocol_ch1	The study was performed with heart tissue isolated from transgenic mouse lines expressing FASN under control of the myocardium-specific alpha-MHC promoter. Two different transgenic lines with high and low level of cardiac FASN expression, respectively, were used. The third group consisted of age-matched B6 control mice.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from heart tissue with the RNeasy Midi kit according to the manufacturer`s instructions (Qiagen).
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA was prepared according to the protocol of the manufacturer (Affymetrix; GeneChip Expression Analysis Technical Manual; Rev. 5; and Invitrogen manual for SuperScript One-Cycle cDNA Kit).
Sample_hyb_protocol	For hybridization, 15 µg of fragmented cRNA were incubated with the chip (Mouse Genome MG430 2.0 Array) in 200 µl of the hybrization solution in a Hybridization Oven 640 (Affymetrix) for 16 h at 45 °C. GeneChips were then washed and stained using the Affymetrix Fluidics Station 450 according to the GeneChip Expression Analysis Technical Manual (Rev. 5).
Sample_scan_protocol	GeneChips were scanned using the Affymetrix GeneChip Scanner 7G.
Sample_data_processing	The signals were processed using Affymetrix GeneChip Operating Software (GCOS; v.1.4; Affymetrix). To compare samples and experiments, the trimmed mean signal of each array was scaled to a target intensity of 300.
Sample_platform_id	GPL1261
Sample_contact_name	Ursula,,Quitterer
Sample_contact_email	ursula.quitterer@pharma.ethz.ch
Sample_contact_phone	0041 44 635 6001
Sample_contact_fax	0041 44 635 6881
Sample_contact_department	Molecular Pharmacology
Sample_contact_institute	Swiss Federal Institute of Technology
Sample_contact_address	Winterthurerstrasse 190
Sample_contact_city	Zurich
Sample_contact_zip/postal_code	CH-8057
Sample_contact_country	Switzerland
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197831/suppl/GSM1197831_B6-2.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1197nnn/GSM1197831/suppl/GSM1197831_B6-2.CHP.gz
Sample_series_id	GSE49351
Sample_data_row_count	45101

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