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Search results for the GEO ID: GSE49392

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Source name

Description

Characteristics

GSM1198735

GPL1355

Wildtype ST14A Cells_5µM MIND4_Biological Replicate 1

Wildtype ST14A Cells, Differentiated 24 hours, 5µM MIND4 Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated wildtype ST14A cells treated with 5µM MIND4

Sample_geo_accession	GSM1198735
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198735/suppl/GSM1198735_1_wt_5uM_A.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198736

GPL1355

Wildtype ST14A Cells_5µM MIND4_Biological Replicate 2

Wildtype ST14A Cells, Differentiated 24 hours, 5µM MIND4 Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated wildtype ST14A cells treated with 5µM MIND4

Sample_geo_accession	GSM1198736
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198736/suppl/GSM1198736_2_wt_5uM_B.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198737

GPL1355

Wildtype ST14A Cells_DMSO_Biological Replicate 1

Wildtype ST14A Cells, Differentiated 24 hours, DMSO Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated wildtype ST14A cells treated with DMSO

Sample_geo_accession	GSM1198737
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198737/suppl/GSM1198737_5_wt_DMSO_A.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198738

GPL1355

Wildtype ST14A Cells_DMSO_Biological Replicate 2

Wildtype ST14A Cells, Differentiated 24 hours, DMSO Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated wildtype ST14A cells treated with DMSO

Sample_geo_accession	GSM1198738
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198738/suppl/GSM1198738_6_wt_DMSO_B.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198739

GPL1355

HD mutant ST14A Cells_5µM MIND4_Biological Replicate 1

HD mutant ST14A Cells, Differentiated 24 hours, 5µM MIND4 Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated HD mutant ST14A cells treated with 5µM MIND4

Sample_geo_accession	GSM1198739
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198739/suppl/GSM1198739_7_mtt_5uM_A.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198740

GPL1355

HD mutant ST14A Cells_5µM MIND4_Biological Replicate 2

HD mutant ST14A Cells, Differentiated 24 hours, 5µM MIND4 Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated HD mutant ST14A cells treated with 5µM MIND4

Sample_geo_accession	GSM1198740
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198740/suppl/GSM1198740_8_mtt_5uM_B.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198741

GPL1355

HD mutant ST14A Cells_DMSO_Biological Replicate 1

HD mutant ST14A Cells, Differentiated 24 hours, DMSO Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated HD mutant ST14A cells treated with DMSO

Sample_geo_accession	GSM1198741
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198741/suppl/GSM1198741_13_mtt_DMSO_A.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

GSM1198742

GPL1355

HD mutant ST14A Cells_DMSO_Biological Replicate 2

HD mutant ST14A Cells, Differentiated 24 hours, DMSO Treated.

cell line: ST14A tissue: rat striatal primordia age: embryonic day 14

Gene expression data from differentiated HD mutant ST14A cells treated with DMSO

Sample_geo_accession	GSM1198742
Sample_status	Public on Aug 01 2013
Sample_submission_date	Jul 31 2013
Sample_last_update_date	Aug 01 2013
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Cells were treated with compounds (5µM MIND4 or DMSO) concurrent with induction of neuronal differentiation for 24 hours, as described (Quinti et al., 2010).
Sample_growth_protocol_ch1	ST14A cells were propagated at 33°C in presence of serum. To induce neuronal differentiation cells were serum deprived and cultured at 37°C in presence of N2 supplement (Invitrogen).
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA was extracted from HD mutant and wild type ST14A cells, differentiated for 24 hours and treated with vehicle (DMSO) or with 5µM MIND4, using the RNeasy kit (Qiagen).
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Labeled cRNAs were prepared according to the manufacturer's instructions, according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Fragmented cRNAs were hybridized to Affymetrix GeneChip Rat Genome 230 2.0 microarrays according to the manufacturer's instructions. GeneChips were washed and stained in the Gene Chip Fluidics Station 450.
Sample_scan_protocol	GeneChips were scanned using the Hewlett-Packard Gene Chip Scanner 3000.
Sample_data_processing	Partek Genomics Suite Version 6.6 Beta (6.12.0207) was used for data processing. Only interrogating probes were imported. No probe filtering was done. RMA background correction was done. Quantile Normalization was applied. Log Probes using Base 2. Probeset summarization Median Polish was done.
Sample_platform_id	GPL1355
Sample_contact_name	Leslie,,Thompson
Sample_contact_email	lmthomps@uci.edu
Sample_contact_institute	University of California, Irvine
Sample_contact_address	Biological Sciences III
Sample_contact_city	Irvine
Sample_contact_state	CA
Sample_contact_zip/postal_code	92697
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM1198nnn/GSM1198742/suppl/GSM1198742_14_mtt_DMSO_B.CEL.gz
Sample_series_id	GSE49392
Sample_data_row_count	31099

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