Search results for the GEO ID: GSE5116 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM115510 | GPL570 |
|
MCF-10F, biological rep1
|
breast epithelial cells derived from fibroadenoma
|
Genotype: slightly aneuploid
|
Gene expression data from nontumorigenic ER(-) human breast epithelial cells
|
Sample_geo_accession | GSM115510
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were grown in medium in normal condition without 17-beta-estradiol treatment
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115510/suppl/GSM115510.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115511 | GPL570 |
|
MCF-10F, biological rep2
|
breast epithelial cells derived from fibroadenoma
|
Genotype: slightly aneuploid
|
Gene expression data from nontumorigenic ER(-) human breast epithelial cells
|
Sample_geo_accession | GSM115511
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were grown in medium in normal condition without 17-beta-estradiol treatment
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115511/suppl/GSM115511.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115512 | GPL570 |
|
MCF-10F, biological rep3
|
breast epithelial cells derived from fibroadenoma
|
Genotype: slightly aneuploid
|
Gene expression data from nontumorigenic ER(-) human breast epithelial cells
|
Sample_geo_accession | GSM115512
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Cells were grown in medium in normal condition without 17-beta-estradiol treatment
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115512/suppl/GSM115512.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115513 | GPL570 |
|
trMCF, biological rep1
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from estrogen transformed MCF-10F cells; the transformation phenotype was validated by conoly formation in agar-methocel and ductulogenesis in collagen matrix
|
Sample_geo_accession | GSM115513
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | MCF-10F cells were repeatedly treated by 70nM 17-beta-estradiol for 2 weeks
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115513/suppl/GSM115513.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115514 | GPL570 |
|
trMCF, biological rep2
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from estrogen transformed MCF-10F cells; the transformation phenotype was validated by conoly formation in agar-methocel and ductulogenesis in collagen matrix
|
Sample_geo_accession | GSM115514
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | MCF-10F cells were repeatedly treated by 70nM 17-beta-estradiol for 2 weeks
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115514/suppl/GSM115514.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115515 | GPL570 |
|
trMCF, biological rep3
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from estrogen transformed MCF-10F cells; the transformation phenotype was validated by conoly formation in agar-methocel and ductulogenesis in collagen matrix
|
Sample_geo_accession | GSM115515
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | MCF-10F cells were repeatedly treated by 70nM 17-beta-estradiol for 2 weeks
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115515/suppl/GSM115515.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115516 | GPL570 |
|
bcMCF, biological rep1
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the bsMCF cell clone
|
Sample_geo_accession | GSM115516
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were cloned and expanded
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115516/suppl/GSM115516.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115517 | GPL570 |
|
bcMCF, biological rep2
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the bsMCF cell clone
|
Sample_geo_accession | GSM115517
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were cloned and expanded
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115517/suppl/GSM115517.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115518 | GPL570 |
|
bcMCF, biological rep3
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the bsMCF cell clone
|
Sample_geo_accession | GSM115518
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were cloned and expanded
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115518/suppl/GSM115518.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115519 | GPL570 |
|
caMCF, biological rep1
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the cell lines established from solid tumors in SCID mice after transplatation of bsMCF cells
|
Sample_geo_accession | GSM115519
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were subcutaneously injected into SCID mice. Solid tumors were grown after transplantation. Cells were derived from the tumor fragments by digestion in enzyme.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115519/suppl/GSM115519.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115520 | GPL570 |
|
caMCF, biological rep2
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the cell lines established from solid tumors in SCID mice after transplatation of bsMCF cells
|
Sample_geo_accession | GSM115520
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were subcutaneously injected into SCID mice. Solid tumors were grown after transplantation. Cells were derived from the tumor fragments by digestion in enzyme.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115520/suppl/GSM115520.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
|
GSM115521 | GPL570 |
|
caMCF, biological rep3
|
MCF-10F cells transformed by 17-beta-estradiol
|
Genotype: slightly aneuploid
|
Gene expression data from the cell lines established from solid tumors in SCID mice after transplatation of bsMCF cells
|
Sample_geo_accession | GSM115521
| Sample_status | Public on Aug 10 2006
| Sample_submission_date | Jun 21 2006
| Sample_last_update_date | Jul 31 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | bsMCF cells were subcutaneously injected into SCID mice. Solid tumors were grown after transplantation. Cells were derived from the tumor fragments by digestion in enzyme.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 8 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
| Sample_data_processing | The data were analyzed with dChip softwareusing invariant-set normalization method.
| Sample_platform_id | GPL570
| Sample_contact_name | Yong,,Huang
| Sample_contact_email | yongh@uchicago.edu
| Sample_contact_phone | (773) 834 0832
| Sample_contact_department | Medicine
| Sample_contact_institute | University of Chicago
| Sample_contact_address | 5841 South Maryland Avenue
| Sample_contact_city | Chicago
| Sample_contact_state | IL
| Sample_contact_zip/postal_code | 60637
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM115nnn/GSM115521/suppl/GSM115521.CEL.gz
| Sample_series_id | GSE5116
| Sample_data_row_count | 54675
| |
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