Search results for the GEO ID: GSE5145 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM116101 | GPL570 |
|
hBSMC_vitamin D_rep1
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human bronchial smooth muscle cell_VitD_100nM_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with 100 nM of vitamin D (1alpha,25-Dihydroxyvitamin D3) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116101
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with 100 nM of 1alpha,25-Dihydroxyvitamin D3 (Sigma, Saint Louis, Missouri).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116101/suppl/GSM116101.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
|
GSM116102 | GPL570 |
|
hBSMC_vitamin D_rep2
|
human bronchial smooth muscle cell_VitD_100nM_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with 100 nM of vitamin D (1alpha,25-Dihydroxyvitamin D3) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116102
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with 100 nM of 1alpha,25-Dihydroxyvitamin D3 (Sigma, Saint Louis, Missouri).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116102/suppl/GSM116102.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
|
GSM116103 | GPL570 |
|
hBSMC_vitamin D_rep3
|
human bronchial smooth muscle cell_VitD_100nM_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with 100 nM of vitamin D (1alpha,25-Dihydroxyvitamin D3) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116103
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with 100 nM of 1alpha,25-Dihydroxyvitamin D3 (Sigma, Saint Louis, Missouri).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116103/suppl/GSM116103.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
|
GSM116104 | GPL570 |
|
hBSMC_control_rep1
|
human bronchial smooth muscle cell_vehicle_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with vehicle (0.05% ethanol) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116104
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with vehicle (ethanol at 0.05%).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116104/suppl/GSM116104.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
|
GSM116105 | GPL570 |
|
hBSMC_control_rep2
|
human bronchial smooth muscle cell_vehicle_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with vehicle (0.05% ethanol) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116105
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with vehicle (ethanol at 0.05%).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116105/suppl/GSM116105.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
|
GSM116106 | GPL570 |
|
hBSMC_control_rep3
|
human bronchial smooth muscle cell_vehicle_24h
|
ethnicity: Hispanic
Gender: Male
Age: 31
Tissue: primary human bronchial smooth muscle cells
|
Human primary bronchial smooth muscle cells were grown to near confluence, starved for 24 hours (medium containing 0.1% FBS) and then treated with vehicle (0.05% ethanol) for an additional 24 hours before RNA extraction.
|
Sample_geo_accession | GSM116106
| Sample_status | Public on Feb 20 2007
| Sample_submission_date | Jun 23 2006
| Sample_last_update_date | Aug 15 2013
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | Cambrex, East Rutherford, New Jersey
| Sample_treatment_protocol_ch1 | BSMCs were grown in 25 cm2 flasks in SmGM and starved for 24 hours (SmBM supplemented with 0.1% FBS) before stimulation. Supernatant was removed and cells were incubated for 24 hours in fresh growth factor-free medium with vehicle (ethanol at 0.05%).
| Sample_growth_protocol_ch1 | Cells were growth in Smooth muscle Growth Medium (SmGM-2 Bulletkit, Cambrex) which included Smooth muscle Basal Medium (SmBM) supplemented with 5 % Fetal Bovine Serum (FBS), hEGF (0.5 ng/ml), hFGF (2 ng/ml), insulin (5 µg/ml) and GA-1000 (100 ng/ml of Gentamicin and 0.1 ng/ml of Amphotericin-B), according to manufacturer's instructions.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Cells were lysed by adding 2 ml of trizol reagent (Invitrogen) and homogenized by passing through a syringe with a needle (26G). RNA was picked up in the aqueous phase following phase separation by chloroform. The RNA was then precipitate with isopropanol, washed with ethanol (75%), dissolved in DEPC-treated RNAse/DNAse free water and quantified by spectrophotometry (Ultrospec 2100 pro).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA was prepared according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_hyb_protocol | Hybridization was performed according to the standard Affymetrix protocol (see complete protocol descriptions: http://www.affymetrix.com/support/technical/manual/expression_manual.affx)
| Sample_scan_protocol | Arrays were scanned with the Affymetrix GeneChip® Scanner 3000 7G and the data was extracted using Microarray Analysis Suite 5.0 (Affymetrix).
| Sample_data_processing | Expression values were extracted using the Robust Multichip Average method (Irizarry RA, Hobbs B, Collin F, et al. 2003. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4: 249-64)
| Sample_platform_id | GPL570
| Sample_contact_name | Yohan,,Bossé
| Sample_contact_email | yohan.bosse@crhl.ulaval.ca
| Sample_contact_phone | 418-656-8711 3725
| Sample_contact_fax | 418-656-4602
| Sample_contact_institute | Laval University
| Sample_contact_address | 2725, chemin Sainte-Foy
| Sample_contact_city | Quebec
| Sample_contact_state | Quebec
| Sample_contact_zip/postal_code | G1V 4G5
| Sample_contact_country | Canada
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM116nnn/GSM116106/suppl/GSM116106.CEL.gz
| Sample_relation | Reanalyzed by: GSE49910
| Sample_series_id | GSE5145
| Sample_data_row_count | 54675
| |
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