Search results for the GEO ID: GSE5679  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM132919 | GPL570 |
|
DC1
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM132919
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF, cytokine treatment was repeated at day 3.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132919/suppl/GSM132919.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132921 | GPL570 |
|
DC1 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Sample_geo_accession | GSM132921
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotine
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132921/suppl/GSM132921.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132922 | GPL570 |
|
DC1 AM
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:female; age: 19 yr; weight: 65 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132922
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100 nM AM580 (AM). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotine
| | Sample_label_protocol_ch1 | cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132922/suppl/GSM132922.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132923 | GPL570 |
|
DC2
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132923
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotine
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132923/suppl/GSM132923.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132924 | GPL570 |
|
DC2 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132924
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132924/suppl/GSM132924.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132925 | GPL570 |
|
DC2 AM
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132925
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100 nM AM580 (AM). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132925/suppl/GSM132925.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132926 | GPL570 |
|
DC2 RSG AGN
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 47 yr; weight: 80 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132926
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG) along with 1 uM AGN193109 (AGN). Ligands or vehicle were added to the cell culture starting from the first day. AGN193109 administration was repeated at day 3.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132926/suppl/GSM132926.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132927 | GPL570 |
|
DC3
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132927
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132927/suppl/GSM132927.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132928 | GPL570 |
|
DC3 RSG
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132928
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132928/suppl/GSM132928.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
| | |
|
GSM132929 | GPL570 |
|
DC3 AM
|
Monocyte derived dendritic cells
|
tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
|
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
|
| Sample_geo_accession | GSM132929
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 100 nM AM580 (AM). Ligands or vehicle were added to the cell culture starting from the first day.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132929/suppl/GSM132929.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
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GSM132930 | GPL570 |
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DC3 RSG AGN
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Monocyte derived dendritic cells
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tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
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Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec).
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| Sample_geo_accession | GSM132930
| | Sample_status | Public on Sep 19 2006
| | Sample_submission_date | Aug 31 2006
| | Sample_last_update_date | Aug 15 2013
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were treated with 2.5 uM rosiglitazone (RSG) along with 1 uM AGN193109 (AGN). Ligands or vehicle were added to the cell culture starting from the first day. AGN193109 administration was repeated at day 3.
| | Sample_growth_protocol_ch1 | Monocytes were resuspended into six-well culture dishes at a density of 1.5x106 cells/ml and cultured in RPMI 1640 (Sigma) supplemented with 10% Fetal bovine serum (FBS) (Invitrogen), containing 800 U/ml GM-CSF (Peprotech) and 500 U/ml IL-4 (Peprotech).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | cRNA was generated from 5 ug of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit.
| | Sample_hyb_protocol | standard Affymetrix protocol
| | Sample_scan_protocol | standard Affymetrix protocol
| | Sample_data_processing | Affymetrix GCOS 1.4, statistical (MAS5) algorithm
| | Sample_platform_id | GPL570
| | Sample_contact_name | Istvan,,Szatmari
| | Sample_contact_email | szatmari@indi.biochem.dote.hu
| | Sample_contact_phone | 36 52 416-432
| | Sample_contact_fax | 36 52 314-989
| | Sample_contact_laboratory | Nuclear Hormone Receptor
| | Sample_contact_department | Department of Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Debrecen
| | Sample_contact_address | Egyetem tér 1.
| | Sample_contact_city | Debrecen
| | Sample_contact_zip/postal_code | H-4010
| | Sample_contact_country | Hungary
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM132nnn/GSM132930/suppl/GSM132930.CEL.gz
| | Sample_relation | Reanalyzed by: GSE49910
| | Sample_series_id | GSE5679
| | Sample_data_row_count | 54675
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