Result

Search results for the GEO ID: GSE5741

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GPL ID

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Title

Source name

Description

Characteristics

GSM133871

GPL570

non-treatment, biological rep1

APRE-19 cells without any treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells without any treatment.

Sample_geo_accession	GSM133871
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133871/suppl/GSM133871.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133872

GPL570

non-treatment, biological rep2

APRE-19 cells without any treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells without any treatment.

Sample_geo_accession	GSM133872
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133872/suppl/GSM133872.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133873

GPL570

non-treatment, biological rep3

APRE-19 cells without any treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells without any treatment.

Sample_geo_accession	GSM133873
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133873/suppl/GSM133873.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133874

GPL570

LDL-treatment, biological rep1

APRE-19 cells with LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with LDL treatment.

Sample_geo_accession	GSM133874
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133874/suppl/GSM133874.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133875

GPL570

LDL-treatment, biological rep2

APRE-19 cells with LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with LDL treatment.

Sample_geo_accession	GSM133875
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133875/suppl/GSM133875.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133876

GPL570

LDL-treatment, biological rep3

APRE-19 cells with LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with LDL treatment.

Sample_geo_accession	GSM133876
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133876/suppl/GSM133876.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133877

GPL570

OX-LDL-treatment, biological rep1

APRE-19 cells with OX-LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with OX-LDL treatment.

Sample_geo_accession	GSM133877
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.OX-LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133877/suppl/GSM133877.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133878

GPL570

OX-LDL-treatment, biological rep2

APRE-19 cells with OX-LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with OX-LDL treatment.

Sample_geo_accession	GSM133878
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.OX-LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133878/suppl/GSM133878.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

GSM133879

GPL570

OX-LDL-treatment, biological rep3

APRE-19 cells with OX-LDL treatment

nonimmortalized human RPE cell line ARPE-19

human APRE cell line APRE-19 cells with OX-LDL treatment.

Sample_geo_accession	GSM133879
Sample_status	Public on Sep 01 2006
Sample_submission_date	Sep 01 2006
Sample_last_update_date	Sep 01 2006
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Homo sapiens
Sample_taxid_ch1	9606
Sample_treatment_protocol_ch1	ARPE-19 cells were seeded at 100,000/cm2 in T-75 cm2 flasks containing Dulbecco’s Modified Eagle medium with 15 mM Hepes buffer (DMEM; Invitrogen-Gibco BRL, Gaithersberg, MD) and 10% fetal bovine serum (FBS; Invitrogen-Gibco BRL) and 2 mM L-glutamine solution, (Invitrogen-Gibco BRL) at 37oC for 1 week, and serum withdrawn in DMEM+1% bovine serum albumin (BSA) for 3 days to make ARPE-19 cells quiescent11.OX-LDL (Intracel, Frederick, MD was added to the medium for 24 hours.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Sample_label_ch1	biotin
Sample_label_protocol_ch1	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Sample_hyb_protocol	Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Sample_scan_protocol	Affymetrix G3000 GeneArray Scanner
Sample_data_processing	Fluorescence was detected using the Affymetrix G3000 GeneArray Scanner and image analysis of each GeneChip was done through the GeneChip Operating System 1.1.1 (GCOS) software from Affymetrix, using the standard default settings.The details of data nalysis are described in paper.
Sample_platform_id	GPL570
Sample_contact_name	Yanqin,,Yang
Sample_contact_institute	The Johns Hopkins University
Sample_contact_address	600 N. Wolfe Street
Sample_contact_city	Baltimore
Sample_contact_state	MD
Sample_contact_zip/postal_code	21287
Sample_contact_country	USA
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM133nnn/GSM133879/suppl/GSM133879.CEL.gz
Sample_series_id	GSE5741
Sample_data_row_count	54675

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