Search results for the GEO ID: GSE5788 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM135264 | GPL96 |
|
T-cell prolymphocytic leukemia, inv(14)(q11q32), case 1
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: inv(14)(q11q32)
Age: 49 years
Gender: male
|
Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
|
Sample_geo_accession | GSM135264
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135264/suppl/GSM135264.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135265 | GPL96 |
|
T-cell prolymphocytic leukemia, inv(14)(q11q32), case 2
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: inv(14)(q11q32)
Age: 54 years
Gender: female
|
Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
|
Sample_geo_accession | GSM135265
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135265/suppl/GSM135265.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135266 | GPL96 |
|
T-cell prolymphocytic leukemia, inv(14)(q11q32), case 3
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: inv(14)(q11q32)
Age: 72 years
Gender: male
|
Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
|
Sample_geo_accession | GSM135266
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135266/suppl/GSM135266.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135267 | GPL96 |
|
T-cell prolymphocytic leukemia, inv(14)(q11q32), case 4
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: inv(14)(q11q32)
Age: 90 years
Gender: female
|
Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
|
Sample_geo_accession | GSM135267
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135267/suppl/GSM135267.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135268 | GPL96 |
|
T-cell prolymphocytic leukemia, inv(14)(q11q32), case 5
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: inv(14)(q11q32)
Age: 74 years
Gender: female
|
Gene expression data from a highly purified inv(14)-positive T-PLL blood sample
|
Sample_geo_accession | GSM135268
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135268/suppl/GSM135268.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135269 | GPL96 |
|
T-cell prolymphocytic leukemia, no inv(14)(q11q32), case 8
|
CD3-purified cells from T-PLL peripheral blood sample. >95% purity
|
Karyotype: no inv(14)(q11q32)
Age: 62 years
Gender: male
|
Gene expression data from a highly purified inv(14)-negative T-PLL blood sample
|
Sample_geo_accession | GSM135269
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135269/suppl/GSM135269.CEL.gz
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135270 | GPL96 |
|
Normal T-cells, CD3+, donor 1
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135270
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135270/suppl/GSM135270.CEL.gz
| Sample_relation | Reanalyzed by: GSM474669
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135271 | GPL96 |
|
Normal T-cells, CD3+, donor 2
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135271
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135271/suppl/GSM135271.CEL.gz
| Sample_relation | Reanalyzed by: GSM474670
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135272 | GPL96 |
|
Normal T-cells, CD3+, donor 3
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135272
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135272/suppl/GSM135272.CEL.gz
| Sample_relation | Reanalyzed by: GSM474671
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135273 | GPL96 |
|
Normal T-cells, CD3+, donor 4
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135273
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135273/suppl/GSM135273.CEL.gz
| Sample_relation | Reanalyzed by: GSM474672
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135274 | GPL96 |
|
Normal T-cells, CD3+, donor 5
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135274
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135274/suppl/GSM135274.CEL.gz
| Sample_relation | Reanalyzed by: GSM474673
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135275 | GPL96 |
|
Normal T-cells, CD3+, donor 6
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135275
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135275/suppl/GSM135275.CEL.gz
| Sample_relation | Reanalyzed by: GSM474674
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135276 | GPL96 |
|
Normal T-cells, CD3+, donor 7
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135276
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135276/suppl/GSM135276.CEL.gz
| Sample_relation | Reanalyzed by: GSM474675
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
|
GSM135277 | GPL96 |
|
Normal T-cells, CD3+, donor 8
|
CD3-purified cells from normal peripheral blood sample. >95% purity
|
Karyotype: normal
Age: unknown
Gender: unknown
|
Gene expression data from immunomagnetically purified CD3+ normal donor derived peripheral blood cells
|
Sample_geo_accession | GSM135277
| Sample_status | Public on Aug 27 2007
| Sample_submission_date | Sep 07 2006
| Sample_last_update_date | Aug 27 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | mononuclear cells were isolated from fresh peripheral blood samples (Lyphoprep, Invitrogen, Karlsruhe, Germany). CD3-positive cells were enriched employing anti-CD3 magnetic microbeads (MIdiMACS, Miltenyi, Bergisch-Gladbach, Germany)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Qiagen RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 13.5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1000.
| Sample_platform_id | GPL96
| Sample_contact_name | Ludger,,Klein-Hitpass
| Sample_contact_email | ludger.klein-hitpass@uni-essen.de
| Sample_contact_phone | +49 201 723 85552
| Sample_contact_laboratory | BioChip Lab
| Sample_contact_department | Universitaetsklinikum
| Sample_contact_institute | Institut fuer Zellbiologie
| Sample_contact_address | Virchowstr. 173
| Sample_contact_city | Essen
| Sample_contact_zip/postal_code | D-45122
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM135nnn/GSM135277/suppl/GSM135277.CEL.gz
| Sample_relation | Reanalyzed by: GSM474676
| Sample_series_id | GSE5788
| Sample_data_row_count | 22283
| |
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