Search results for the GEO ID: GSE5968  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM138622 | GPL570 |
|
2x9 PGC-1 mutant, repeat 1
|
HePG2
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the 2x9 mutant of PGC-1
|
| Sample_geo_accession | GSM138622
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified inthe title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138622/suppl/GSM138622.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138623 | GPL570 |
|
2x9 PGC-1 mutant, repeat 2
|
HePG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the 2x9 mutant of PGC-1
|
| Sample_geo_accession | GSM138623
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified inthe title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138623/suppl/GSM138623.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138624 | GPL570 |
|
2x9 PGC-1 mutant, repeat 3
|
HePG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the 2x9 mutant of PGC-1
|
| Sample_geo_accession | GSM138624
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified inthe title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138624/suppl/GSM138624.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138625 | GPL570 |
|
BGAL, repeat 1
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the BGAL control
|
| Sample_geo_accession | GSM138625
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138625/suppl/GSM138625.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138626 | GPL570 |
|
BGAL, repeat 2
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the BGAL control
|
| Sample_geo_accession | GSM138626
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138626/suppl/GSM138626.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138627 | GPL570 |
|
BGAL, repeat 3
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the BGAL control
|
| Sample_geo_accession | GSM138627
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138627/suppl/GSM138627.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138628 | GPL570 |
|
L2L3 mutant, repeat 1
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the L2L3 mutant of PGC-1
|
| Sample_geo_accession | GSM138628
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138628/suppl/GSM138628.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138629 | GPL570 |
|
L2L3 mutant, repeat 2
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the L2L3 mutant of PGC-1
|
| Sample_geo_accession | GSM138629
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138629/suppl/GSM138629.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138630 | GPL570 |
|
L2L3 mutant, repeat 3
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing the L2L3 mutant of PGC-1
|
| Sample_geo_accession | GSM138630
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138630/suppl/GSM138630.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138631 | GPL570 |
|
wt PGC-1, repeat 1
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing wt PGC-1
|
| Sample_geo_accession | GSM138631
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138631/suppl/GSM138631.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138632 | GPL570 |
|
wt PGC-1, repeat 2
|
HepG2 cells
|
Human hepatocellular carcinoma cell line
|
HePG2 cells infected with adenovirus expressing wt PGC-1
|
| Sample_geo_accession | GSM138632
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138632/suppl/GSM138632.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
| | |
|
GSM138633 | GPL570 |
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wt PGC-1, repeat 3
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HepG2 cells
|
Human hepatocellular carcinoma cell line
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HePG2 cells infected with adenovirus expressing wt PGC-1
|
| Sample_geo_accession | GSM138633
| | Sample_status | Public on Nov 10 2006
| | Sample_submission_date | Oct 04 2006
| | Sample_last_update_date | Oct 04 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | 4,000,000 cells were plated per 10 cm plate coated with gelatin (0.1% gelatin for 10 min). Cells were infected on the next day with adenovirus expressing the construct identified in the title. MOI=10 to 30. Infected for 2 hrs at RT in 0.5 mL phenol-free charcoal filtered MEM (8% serum), after which the medium was repaced with 2 mL of phenol red-free MEM. Plates were incubated at 37 C 5% CO2 for 24 hrs, and cells were harvested for RNA isolation immediately following this incubation.
| | Sample_growth_protocol_ch1 | HePG2 cells were maintained in MEM supplemented with 8% FBS, non-essential aminoacids and sodium pyruvate on plastic coated with 0.1% gelatin.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | RNEASY (Qiagen)
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_hyb_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_scan_protocol | As per standard Affymetrix protocol (performed at Expression Analysis Institute, Durham, NC)
| | Sample_data_processing | MAS5.0
| | Sample_platform_id | GPL570
| | Sample_contact_name | Dmitri,A,Kazmin
| | Sample_contact_email | kazmi002@mc.duke.edu
| | Sample_contact_phone | 919-681-1348
| | Sample_contact_fax | 919-681-7139
| | Sample_contact_laboratory | McDonnell
| | Sample_contact_department | Pharmacology and Cancer Biology
| | Sample_contact_institute | Duke University Medical Center
| | Sample_contact_address | C264 LSRC, Research Dr
| | Sample_contact_city | Durham
| | Sample_contact_state | NC
| | Sample_contact_zip/postal_code | 27710
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM138nnn/GSM138633/suppl/GSM138633.CEL.gz
| | Sample_series_id | GSE5968
| | Sample_data_row_count | 54675
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