Result

Search results for the GEO ID: GSE5996

(Click on the check boxes provided under "Select for analysis", to initiate grouping)

(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down)

GSM ID

GPL ID

Select for analysis

Title

Source name

Description

Characteristics

GSM139268

GPL1355

Cardiomyocyte control 1

isolated neonatal cardiomyocytes from rat heart

control condition

control condition 1

Sample_geo_accession	GSM139268
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139268/suppl/GSM139268.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

GSM139269

GPL1355

Cardiomyocyte control 2

isolated neonatal cardiomyocytes from rat heart

control condition

control condition 2

Sample_geo_accession	GSM139269
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139269/suppl/GSM139269.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

GSM139270

GPL1355

Cardiomyocyte phenylephrine 1

isolated neonatal cardiomyocytes from rat heart

induced by phenylephrine

phenylephrine condition 1

Sample_geo_accession	GSM139270
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Pharmacological stimulation was carried out with phenylephrine (PE; Sigma), 50 umol/l, endothelin-1 (ET-1; Sigma), 100 nM, and Angiotensin-II (Ang-II; Calbiochem, Darmstadt, Germany), 100 nM, respectively.
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139270/suppl/GSM139270.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

GSM139271

GPL1355

Cardiomyocyte phenylephrine 2

isolated neonatal cardiomyocytes from rat heart

induced by phenylephrine

phenylephrine condition 2

Sample_geo_accession	GSM139271
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	Pharmacological stimulation was carried out with phenylephrine (PE; Sigma), 50 umol/l, endothelin-1 (ET-1; Sigma), 100 nM, and Angiotensin-II (Ang-II; Calbiochem, Darmstadt, Germany), 100 nM, respectively.
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139271/suppl/GSM139271.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

GSM139272

GPL1355

Cardiomyocyte stretch 1

isolated neonatal cardiomyocytes from rat heart

induced by biomechanical stretching

stretch condition 1

Sample_geo_accession	GSM139272
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	A transparent silicone membrane (0.25 mm, Specialty Manufacturing, Saginaw, MI, USA) was attached to a 60 mm biaxial stretch device as described previously and coated with a 0,1 mg/ml collagen type I solution (Sigma, Munich, Germany). NRVCMs were seeded out at a density of 1.75 x 10^5 cells per cm2, resulting in a total cell count of 1 x 10^7 cells per stretch device. After 36 h, cells were washed with PBS and serum starved in DMEM medium for another 24 h until biaxial stretch was applied to a total of 112% for the indicated period of time.
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139272/suppl/GSM139272.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

GSM139273

GPL1355

Cardiomyocyte stretch 2

isolated neonatal cardiomyocytes from rat heart

induced by biomechanical stretch

stretch condition 2

Sample_geo_accession	GSM139273
Sample_status	Public on Dec 01 2007
Sample_submission_date	Oct 09 2006
Sample_last_update_date	May 25 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_treatment_protocol_ch1	A transparent silicone membrane (0.25 mm, Specialty Manufacturing, Saginaw, MI, USA) was attached to a 60 mm biaxial stretch device as described previously and coated with a 0,1 mg/ml collagen type I solution (Sigma, Munich, Germany). NRVCMs were seeded out at a density of 1.75 x 10^5 cells per cm2, resulting in a total cell count of 1 x 10^7 cells per stretch device. After 36 h, cells were washed with PBS and serum starved in DMEM medium for another 24 h until biaxial stretch was applied to a total of 112% for the indicated period of time.
Sample_growth_protocol_ch1	Hearts from 1-2 days old Wistar rats (Charles River, Sulzfeld, Germany) were harvested and minced in PBS. Subsequently, up to six digestion steps were carried out with pancreatin (Sigma, Munich, Germany, 0,6 mg/ml) and collagenase type II (Worthington, 0,5 mg/ml) in sterile ADS buffer containing 120 mmol/l NaCl, 20 mmol/l HEPES, 8 mmol/l NaH2PO4, 6 mmol/l glucose, 5 mmol/l KCl, 0.8 mmol/l MgSO4, pH 7.4. Cardiomyocytes were purified from contaminating fibroblasts using a Percoll (Amersham, Germany) gradient centrifugation step. Finally, NRVCMs were resuspended and cultered in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% FCS, penicillin/streptomycin and L-glutamine (all from PAA, Linz, Austria) .
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA was isolated from NRVCMs using the TRIzolTM reagent (Invitrogen, Germany) according to the manufacturer’s protocol. RNA was resuspended in DEPC (Sigma)–treated H2O. RNA integrity and purity were electrophoretically verified by ethidium bromide staining and measurement of OD260/OD280 absorption ratios. Total RNA was pooled from several preparations and purified from contaminating genomic DNA by a DNase I digestion (Sigma, AMPD1).
Sample_label_ch1	biotin + phycoerythrin-streptavidin
Sample_label_protocol_ch1	Affymetrix expression profiling was carried out at the German Ressource Center for Genome Research (RZPD; Berlin, Germany). Briefly, integrity of RNA was checked using an Agilent Bioanalyzer (Eukaryote total RNA assay) followed by cDNA synthesis and in vitro transcription with Ambion’s Message Amp kit according the manufacturer’s instructions. cRNA was subjected to quality control using the Bioanalyzer with the mRNA smear nano assay. Final cRNA concentration was 60 ng/µl.
Sample_hyb_protocol	Hybridization was carried out for 16-18 hours in the Affymetrix Oven 640 at 60 rpm and 45°C. Subsequent washing and staining was performed with a fluidics station (Gene Chip® Fluidics station 400, Affymetrix) and controlled by Affymetrix GeneChip Operating System (GCOS).
Sample_scan_protocol	The scanning process and primary data analysis was done with the Affymetrix GeneChip Scanner 3000 controlled with GCOS.
Sample_data_processing	variance stabilizing normalization procedure (Huber et al., Bioinformatics 2002)
Sample_platform_id	GPL1355
Sample_contact_name	Derk,,Frank
Sample_contact_email	derk.frank@med.uni-heidelberg.de
Sample_contact_phone	+49-6221-56-39830
Sample_contact_fax	+49-6221-56-4866
Sample_contact_laboratory	AG Frey
Sample_contact_department	Dept. of Internal Medicine III
Sample_contact_institute	University of Heidelberg
Sample_contact_address	Im Neuenheimer Feld 350
Sample_contact_city	Heidelberg
Sample_contact_zip/postal_code	69120
Sample_contact_country	Germany
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM139nnn/GSM139273/suppl/GSM139273.CEL.gz
Sample_series_id	GSE5996
Sample_data_row_count	31099

Make groups for comparisons

(2 groups will be compared at a time)

Select GSMs and click on "Add groups"

Enter the group name here:

Select expression type

Transcripts profile based on;

A. Differential status (Up/Down regulation)

B. Absolute calls (Transcribed/Not-detected)

Filter results by number of probes