Search results for the GEO ID: GSE6075  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM140797 | GPL570 |
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HOK/Bmi-1 with LXSN
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HOK/Bmi-1 cells infected with LXSN
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Normal human oral keratinocytes (NHOK) explanted from normal human gingival tissue; infected with Bmi-1-expressing retrovirus; superinfected with LXSN-rexpressing retrovirus.
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Cells were harvested at the actively proliferating stage.
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| Sample_geo_accession | GSM140797
| | Sample_status | Public on Oct 20 2006
| | Sample_submission_date | Oct 18 2006
| | Sample_last_update_date | Oct 19 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Dr. Park's lab
| | Sample_treatment_protocol_ch1 | Used retroviruses to express Bmi-1 and control vector (LXSN).
| | Sample_growth_protocol_ch1 | Keratinocyte Growth Medium (KGM)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tRNA was isolated from actively proliferating Bmi-1/LXSN cells using Qiagen RNeasy total RNA isolation kit (Qiagen Inc.) according to their protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-labeled antisense cRNA was produced by in vitro transcription using the ENZO BioArray HighYield kit.
| | Sample_hyb_protocol | cRNA (15 ug) was fragmented and hybridized to Affymetrix HG-U133 Plus 2.0.
| | Sample_scan_protocol | The chip was stained with streptavidinphycoerythrin and analyzed using GCOS 1.2 software for pairwise comparison of gene expression.
| | Sample_data_processing | Original "signal" values from Affymetrix GCOS1.2 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Reuben,Han-Kyu,Kim
| | Sample_contact_email | reubenk@ucla.edu
| | Sample_contact_department | Dentistry
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10083 Le Conte Ave.
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90024
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140797/suppl/GSM140797.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140797/suppl/GSM140797.EXP.gz
| | Sample_series_id | GSE6075
| | Sample_data_row_count | 54675
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GSM140798 | GPL570 |
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HOK/Bmi-1 with E6
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HOK/Bmi-1 cells infected with E6
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Normal human oral keratinocytes (NHOK); Normal human oral keratinocytes (NHOK) explanted from normal human gingival tissue; infected with Bmi-1-expressing retrovirus; superinfected with E6 expressing retrovirus.
|
Cells were harvested at the actively proliferating stage.
|
| Sample_geo_accession | GSM140798
| | Sample_status | Public on Oct 20 2006
| | Sample_submission_date | Oct 18 2006
| | Sample_last_update_date | Oct 19 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Dr. Park's lab
| | Sample_treatment_protocol_ch1 | Used retroviruses to express Bmi-1 and E6.
| | Sample_growth_protocol_ch1 | Keratinocyte Growth Medium (KGM)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tRNA was isolated from actively proliferating Bmi-1/E6 cells using Qiagen RNeasy total RNA isolation kit (Qiagen Inc.) according to their protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-labeled antisense cRNA was produced by in vitro transcription using the ENZO BioArray HighYield kit.
| | Sample_hyb_protocol | cRNA (15 ug) was fragmented and hybridized to Affymetrix HG-U133 Plus 2.0.
| | Sample_scan_protocol | The chip was stained with streptavidinphycoerythrin and analyzed using GCOS 1.2 software for pairwise comparison of gene expression.
| | Sample_data_processing | Original "signal" values from Affymetrix GCOS1.2 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Reuben,Han-Kyu,Kim
| | Sample_contact_email | reubenk@ucla.edu
| | Sample_contact_department | Dentistry
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10083 Le Conte Ave.
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90024
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140798/suppl/GSM140798.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140798/suppl/GSM140798.EXP.gz
| | Sample_series_id | GSE6075
| | Sample_data_row_count | 54675
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GSM140799 | GPL570 |
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HOK/Bmi-1 with E6(Delta)118-122.
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HOK/Bmi-1 cells infected with E6(Delta)118-122.
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Normal human oral keratinocytes (NHOK); Normal human oral keratinocytes (NHOK) explanted from normal human gingival tissue; infected with Bmi-1-expressing retrovirus; superinfected with E6(Delta)118-122 expressing retrovirus.
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Cells were harvested at the actively proliferating stage.
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| Sample_geo_accession | GSM140799
| | Sample_status | Public on Oct 20 2006
| | Sample_submission_date | Oct 18 2006
| | Sample_last_update_date | Oct 19 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Dr. Park's lab
| | Sample_treatment_protocol_ch1 | Used retroviruses to express Bmi-1 and E6(Delta)118-122 .
| | Sample_growth_protocol_ch1 | Keratinocyte Growth Medium (KGM)
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | tRNA was isolated from actively proliferating Bmi-1/E6(Delta)118-122 cells using Qiagen RNeasy total RNA isolation kit (Qiagen Inc.) according to their protocols.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Biotin-labeled antisense cRNA was produced by in vitro transcription using the ENZO BioArray HighYield kit.
| | Sample_hyb_protocol | cRNA (15 ug) was fragmented and hybridized to Affymetrix HG-U133 Plus 2.0.
| | Sample_scan_protocol | The chip was stained with streptavidinphycoerythrin and analyzed using GCOS 1.2 software for pairwise comparison of gene expression.
| | Sample_data_processing | Original "signal" values from Affymetrix GCOS1.2 software.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Reuben,Han-Kyu,Kim
| | Sample_contact_email | reubenk@ucla.edu
| | Sample_contact_department | Dentistry
| | Sample_contact_institute | UCLA
| | Sample_contact_address | 10083 Le Conte Ave.
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90024
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140799/suppl/GSM140799.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM140nnn/GSM140799/suppl/GSM140799.EXP.gz
| | Sample_series_id | GSE6075
| | Sample_data_row_count | 54675
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