Search results for the GEO ID: GSE6206  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM143370 | GPL339 |
|
untreated Rb-/- MEFs-1
|
Rb null MEFs without cisplatin treatment
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143370
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143370/suppl/GSM143370.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143371 | GPL339 |
|
untreated Rb-/- MEFs-2
|
Rb null MEFs without cisplatin treatment
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143371
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143371/suppl/GSM143371.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143372 | GPL339 |
|
untreated Rb-/- MEFs-3
|
Rb null MEFs without cisplatin treatment
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143372
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143372/suppl/GSM143372.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143373 | GPL339 |
|
untreated WT MEFs-1
|
wildtype MEFs without cisplatin treatment
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143373
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143373/suppl/GSM143373.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143374 | GPL339 |
|
untreated WT MEFs-2
|
wildtype MEFs without cisplatin treatment
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143374
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143374/suppl/GSM143374.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143375 | GPL339 |
|
untreated WT MEFs-3
|
wildtype MEFs without cisplatin treatment
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs without cisplatin treatment.
|
| Sample_geo_accession | GSM143375
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Upon harvest, cells without cisplatin treatment (controls) were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143375/suppl/GSM143375.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143376 | GPL339 |
|
Cisplatin-treated Rb-/- MEFs-a
|
Rb null MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143376
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143376/suppl/GSM143376.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143377 | GPL339 |
|
Cisplatin-treated Rb-/- MEFs-b
|
Rb null MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143377
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143377/suppl/GSM143377.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143378 | GPL339 |
|
Cisplatin-treated Rb-/- MEFs-c
|
Rb null MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: Rb null MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from Rb null MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143378
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143378/suppl/GSM143378.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143379 | GPL339 |
|
Cisplatin-treated wildtype MEFs-a
|
wildtype MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143379
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143379/suppl/GSM143379.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143380 | GPL339 |
|
Cisplatin-treated wildtype MEFs-b
|
wildtype MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143380
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143380/suppl/GSM143380.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
|
GSM143381 | GPL339 |
|
Cisplatin-treated wildtype MEFs-c
|
wildtype MEFs with cisplatin treatment (16microM, 24hrs)
|
Genotype: wildtype MEFs
Primary mouse embryonic fibroblasts (MEFs) generated from embryos at E13.5
|
Gene expression data from wildtype MEFs with cisplatin treatment (16microM, 24hrs).
|
| Sample_geo_accession | GSM143381
| | Sample_status | Public on Sep 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | May 22 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Cells were treated with 16 microM cisplatin for 24 hrs prior to harvest. Upon harvest, cells were washed with PBS and then scraped from plates in the Trizol solution (GibcoBRL).
| | Sample_growth_protocol_ch1 | MEFs were cultured in DMEM (high glucose, Invitrogen) with 10% FCS (Invitrogen) and passaged upon confluency at the ratio 1:3. Cells were kept in culture before reaching passage 5.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Kay ,,Macleod
| | Sample_contact_email | kmacleod@huggins.bsd.uchicago.edu
| | Sample_contact_phone | 773-834-8309
| | Sample_contact_fax | 773-702-4476
| | Sample_contact_laboratory | Macleod
| | Sample_contact_department | Ben May Institute for Cancer Research
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 E 57th Str, CIS W338
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143381/suppl/GSM143381.CEL.gz
| | Sample_series_id | GSE6206
| | Sample_data_row_count | 22690
| | |
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