Search results for the GEO ID: GSE6208  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
(Once the selection is made, click on "Add groups" in "Make groups for comparison", to make a group. Scroll down) |
|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM143324 | GPL1355 |
|
Dahl S-T0992
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143324
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143324/suppl/GSM143324.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143325 | GPL1355 |
|
Dahl S-T0997
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
Gender: Male
Age: 4 weeks old
Tissue: whole kidney
Diet: low salt (0.3% NaCl)
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143325
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143325/suppl/GSM143325.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143326 | GPL1355 |
|
Dahl S-T1059
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143326
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143326/suppl/GSM143326.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143327 | GPL1355 |
|
S.SHR(2)-50380
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143327
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143327/suppl/GSM143327.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143328 | GPL1355 |
|
S.SHR(2)-50388
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143328
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143328/suppl/GSM143328.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143329 | GPL1355 |
|
S.SHR(2)-50398
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143329
| | Sample_status | Public on Oct 31 2007
| | Sample_submission_date | Oct 31 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143329/suppl/GSM143329.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143354 | GPL1355 |
|
Dahl S-T0996
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
Gender: Male
Age: 12 weeks old
Tissue: whole kidney
Diet: low salt (0.3% NaCl)
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143354
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143354/suppl/GSM143354.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143355 | GPL1355 |
|
Dahl S-T1051
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143355
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143355/suppl/GSM143355.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143356 | GPL1355 |
|
Dahl S-T1010
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143356
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143356/suppl/GSM143356.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143357 | GPL1355 |
|
S.SHR(2)-50376
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143357
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143357/suppl/GSM143357.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143358 | GPL1355 |
|
S.SHR(2)-50386
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143358
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143358/suppl/GSM143358.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143359 | GPL1355 |
|
S.SHR(2)-50605
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143359
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143359/suppl/GSM143359.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143360 | GPL1355 |
|
Dahl S-T1004
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143360
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143360/suppl/GSM143360.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143361 | GPL1355 |
|
Dahl S-T1030
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143361
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143361/suppl/GSM143361.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143362 | GPL1355 |
|
Dahl S-T1087
|
whole kidney from Dahl Salt-Sensitive
|
Strain: SS/Jr
|
Gene expression from Dahl salt-sensitive stain
|
| Sample_geo_accession | GSM143362
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143362/suppl/GSM143362.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143363 | GPL1355 |
|
S.SHR(2)-50379
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143363
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143363/suppl/GSM143363.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143365 | GPL1355 |
|
S.SHR(2)-50594
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143365
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143365/suppl/GSM143365.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
GSM143366 | GPL1355 |
|
S.SHR(2)-50957
|
whole kidney from Renal Protective S.SHR(2) Congenic Strain
|
Strain: Congenic strain derived from SS/Jr and SHR/NHsd
|
Gene expression from Renal Protective S.SHR(2) Congenic Strain
|
| Sample_geo_accession | GSM143366
| | Sample_status | Public on Nov 01 2007
| | Sample_submission_date | Nov 01 2006
| | Sample_last_update_date | Nov 02 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Kidney was cut into ~ 0.5cM size cubes, suspended in RNAlater (Ambion, Austin, TX) and stored overnight at 4ºC. RNA was extracted using Trizol® reagent (Invitrogen, Carlsbad, CA) and purified using Mini RNeasy kit (Qiagen,Valencia, CA ) according to manufacturer’s protocols. RNA quality was assessed by an OD260/280 ratio > 2.0 and visually by ethidium bromide staining on an agarose gel.
| | Sample_label_ch1 | Biotinylated cRNA
| | Sample_label_protocol_ch1 | Biotinylated cRNA was synthesized from 10 ug of total RNA using the One-Cycle Target Labeling Kit (Affymetrix, Santa Clara, CA) as directed by the user manual. cRNA quality for each sample was assessed by hybridization of 5ug adjusted kidney cRNA to Affymetrix Genechip® Test3 array. Subsequently, 15ug adjusted kidney cRNA was hybridized to the Genechip® Rat 230 2.0 array.
| | Sample_hyb_protocol | Hybridized chips were automatically washed and stained and at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_scan_protocol | Genechips® were scanned at the Medical University of Ohio Bioinformatics & Proteomics/Genomics Program gene array facility using Affymetrix equipment and protocols recommended by manufacturer.
| | Sample_data_processing | Absolute analysis of data were conducted using the default settings of the Affymetrix software (GeneChip Software MAS-5.0)
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Michael,R,Garrett
| | Sample_contact_email | michael.garrett2@utoledo.edu
| | Sample_contact_phone | 419-383-4026
| | Sample_contact_fax | 419-383-6168
| | Sample_contact_department | Physiology and Pharmacology
| | Sample_contact_institute | University of Toledo-Health Science Campus
| | Sample_contact_address | 3000 Arlington Ave, MS#1009
| | Sample_contact_city | Toledo
| | Sample_contact_state | OH
| | Sample_contact_zip/postal_code | 43614
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM143nnn/GSM143366/suppl/GSM143366.CEL.gz
| | Sample_series_id | GSE6208
| | Sample_data_row_count | 31099
| | |
|
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| |
Make groups for comparisons |
(2 groups will be compared at a time) |
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| Select GSMs and click on "Add groups" |
Enter the group name here: |
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