Search results for the GEO ID: GSE6283  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM144081 | GPL570 |
|
amniocyte_normalkaryotype_rep1
|
amniocytes, normal karyotype
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: normal
|
amniocyte euploid control sample replicate 1
|
| Sample_geo_accession | GSM144081
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144081/suppl/GSM144081.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144082 | GPL570 |
|
amniocyte_normalkaryotype_rep2
|
amniocytes, normal karyotype
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: normal
|
amniocyte euploid control sample replicate 2
|
| Sample_geo_accession | GSM144082
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144082/suppl/GSM144082.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144084 | GPL570 |
|
amniocyte_normalkaryotype_rep3
|
amniocytes, normal karyotype
|
tissue: prenatal amniocytes
Age: 15 gestational weeks
gender: male
karyotype: normal
|
amniocyte euploid control sample replicate 3
|
| Sample_geo_accession | GSM144084
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144084/suppl/GSM144084.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144087 | GPL570 |
|
chorion villus cells_normalkaryotype_rep1
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: normal
|
chorion villus cells euploid control sample replicate 1
|
| Sample_geo_accession | GSM144087
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144087/suppl/GSM144087.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144088 | GPL570 |
|
chorion villus cells_normalkaryotype_rep2
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: normal
|
chorion villus cells euploid control sample replicate 2
|
| Sample_geo_accession | GSM144088
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144088/suppl/GSM144088.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144089 | GPL570 |
|
amniocytes_trisomy21_rep1
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: trisomy 21
|
amniocytes trisomy 21 replicate 1
|
| Sample_geo_accession | GSM144089
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144089/suppl/GSM144089.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144090 | GPL570 |
|
amniocytes_trisomy21_rep2
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: trisomy 21
|
amniocytes trisomy 21 replicate 2
|
| Sample_geo_accession | GSM144090
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144090/suppl/GSM144090.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144091 | GPL570 |
|
amnoicytes_trisomy21_rep3
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: trisomy 21
|
amniocytes trisomy 21 replicate 3
|
| Sample_geo_accession | GSM144091
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144091/suppl/GSM144091.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144097 | GPL570 |
|
chorion villus cells_trisomy21_rep1
|
chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 14 gestational weeks
gender: male
karyotype: trisomy 21
|
chorion villus cells trisomy 21 replicate 1
|
| Sample_geo_accession | GSM144097
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 09 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplementary Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144097/suppl/GSM144097.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144509 | GPL570 |
|
chorion villus cells_trisomy21_rep2
|
chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: trisomy 21
|
prenatal sample with a trisomy 21
|
| Sample_geo_accession | GSM144509
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144509/suppl/GSM144509.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144511 | GPL570 |
|
chorion villus cells_trisomy21_rep3
|
chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 13 gestational weeks
gender: female
karyotype: trisomy 21
|
prenatal sample with a trisomy 21
|
| Sample_geo_accession | GSM144511
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144511/suppl/GSM144511.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144512 | GPL570 |
|
chorion villus cells_normalkaryotype_rep3
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: normal
|
prenatal sample with a normal karyotype
|
| Sample_geo_accession | GSM144512
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144512/suppl/GSM144512.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144513 | GPL570 |
|
chorion villus cells_normal karyptype_rep5
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: male
karyotype: normal
|
prenatal sample with a normal karyotype
|
| Sample_geo_accession | GSM144513
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144513/suppl/GSM144513.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144514 | GPL570 |
|
amniocytes_trisomy13_rep1
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 15 gestational weeks
gender: male
karyotype: trisomy 13
|
prenatal sample with a translocation-trisomy 13. The translocation involves the whole chromosome 13.
|
| Sample_geo_accession | GSM144514
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144514/suppl/GSM144514.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144515 | GPL570 |
|
amniocytes_trisomy13_rep2
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: female
karyotype: trisomy 13
|
prenatal sample with a trisomy 13.
|
| Sample_geo_accession | GSM144515
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144515/suppl/GSM144515.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144516 | GPL570 |
|
amniocytes_trisomy13_rep3
|
amniocytes, trisomy
|
tissue: prenatal amniocytes
Age: 16 gestational weeks
gender: male
karyotype: trisomy 13
|
prenatal sample with a trisomy 13.
|
| Sample_geo_accession | GSM144516
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max II Complete Medium (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144516/suppl/GSM144516.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144518 | GPL570 |
|
chorion villus cells_normalkaryotype_rep4
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: normal
|
prenatal euploid sample
|
| Sample_geo_accession | GSM144518
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144518/suppl/GSM144518.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144519 | GPL570 |
|
chorion villus cells_normalkaryotype_rep6
|
chorion villus cells, normal karyotype
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: male
karyotype: normal
|
prenatal euploid sample
|
| Sample_geo_accession | GSM144519
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144519/suppl/GSM144519.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144520 | GPL570 |
|
chorion villus cells_trisomy18_rep1
|
chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 13 gestational weeks
gender: male
karyotype: Trisomy 18
|
prenatal sample with a trisomy 18
|
| Sample_geo_accession | GSM144520
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144520/suppl/GSM144520.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144521 | GPL570 |
|
chorion villus cells_trisomy18_rep2
|
chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 13 gestational weeks
gender: female
karyotype: Trisomy 18
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prenatal sample with a trisomy 18
|
| Sample_geo_accession | GSM144521
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Institute for Clinical Genetics, Stuttgart, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144521/suppl/GSM144521.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
|
GSM144522 | GPL570 |
|
chorion villus cells_trisomy18_rep3
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chorion villus cells, trisomy
|
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: Trisomy 18
|
prenatal sample with a trisomy 18
|
| Sample_geo_accession | GSM144522
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 15 2006
| | Sample_last_update_date | Nov 16 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | Department of Medical Genetics of University of Tuebingen, Germany
| | Sample_treatment_protocol_ch1 | No treatment
| | Sample_growth_protocol_ch1 | Primary cell cultures of the amniocytes were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Amniocytes were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Affymetrix standard labeling protocol
| | Sample_hyb_protocol | Affymetrix standard hybridization protocol
| | Sample_scan_protocol | Affymetrix standard scan protocol
| | Sample_data_processing | Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Oezge,,Altug Teber
| | Sample_contact_email | oezge.altug-teber@med.uni-tuebingen.de
| | Sample_contact_phone | 004970712972307
| | Sample_contact_fax | 00497071295172
| | Sample_contact_laboratory | Microarray Facility
| | Sample_contact_department | Medical Genetics
| | Sample_contact_institute | University of Tuebingen
| | Sample_contact_address | Calwerstr. 7
| | Sample_contact_city | Tuebingen
| | Sample_contact_zip/postal_code | 72076
| | Sample_contact_country | Germany
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144522/suppl/GSM144522.CEL.gz
| | Sample_series_id | GSE6283
| | Sample_data_row_count | 54675
| | |
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