Result

Search results for the GEO ID: GSE6287

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM144569

GPL339

renal vesicle - xrv1

renal vesicle

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

>> Amplification protocol << Target Amplified manufacturer/kit: Affymetrix (Enzo)/Arcturus Target Amplified protocol: Arcturus/Affymetrix Rounds of amplification: 2 URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:7056

Sample_geo_accession	GSM144569
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 renal vesicles
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144569/suppl/GSM144569.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144570

GPL339

renal vesicle - xrv2

renal vesicle

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144570
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 renal vesicles
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144570/suppl/GSM144570.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144571

GPL339

renal vesicle - xrv3

renal vesicle

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144571
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 renal vesicles
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144571/suppl/GSM144571.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144572

GPL339

s-shaped body - xs01

s-shaped body

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144572
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 s-shaped bodies
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144572/suppl/GSM144572.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144573

GPL339

s-shaped body - xs02

s-shaped body

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144573
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 s-shaped bodies
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144573/suppl/GSM144573.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144574

GPL339

s-shaped body - xs03

s-shaped body

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144574
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 20 s-shaped bodies
Sample_treatment_protocol_ch1	Pooled sample: Yes
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144574/suppl/GSM144574.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144575

GPL339

collecting duct - xur1

collecting duct

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144575
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 1 collecting duct
Sample_treatment_protocol_ch1	Pooled sample: No
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144575/suppl/GSM144575.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144576

GPL339

collecting duct - xur2

collecting duct

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144576
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 1 collecting duct
Sample_treatment_protocol_ch1	Pooled sample: No
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144576/suppl/GSM144576.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

GSM144577

GPL339

collecting duct - xur3

collecting duct

gene reported: wild-type strain: 129 Sex: unknown developmental stage: E14.5 theiler stage: 22 somite count: NA developmental landmark: NA

Sample_geo_accession	GSM144577
Sample_status	Public on Nov 18 2006
Sample_submission_date	Nov 15 2006
Sample_last_update_date	Dec 29 2010
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Mus musculus
Sample_taxid_ch1	10090
Sample_treatment_protocol_ch1	pool size: 1 collecting duct
Sample_treatment_protocol_ch1	Pooled sample: No
Sample_treatment_protocol_ch1	Dissection Method: Whole organ excision
Sample_treatment_protocol_ch1	Two early nephron structures, the renal vesicle and the s-shaped body, and the collecting duct system, were microdissected from E14.5 kidneys based on morphological criteria. Antibody and lectin staining together with RT-PCR were used for identity verification and for exclusion of contamination by other tissues. Samples are minimally pooled for amplification. A biological replicate is a single minimally pooled sample. RNA isolated from the collected structures was put through two rounds of T7-driven amplification to obtain the microgram quantities of cRNA needed for hybridization to Affymetrix microarrays.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	Total RNA extracted using TRIzol. Two round amplification using the Arcturus kit according to the manufacturer protocol.
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	Affymetrix standard protocol
Sample_hyb_protocol	Amount labeled target hybridization to array: 15 ug
Sample_hyb_protocol	Hybridization/Wash/Stain protocol: EukGE-WS2v4
Sample_scan_protocol	Affymetrix standard protocol
Sample_data_processing	Analysis method: Affymetrix GCOS and Rosetta Resolver GCOS Tgt value: 500
Sample_platform_id	GPL339
Sample_contact_name	GUDMAP,,Developers
Sample_contact_email	gudmap-editors@gudmap.org
Sample_contact_phone	+44 131 3322471
Sample_contact_laboratory	GUDMAP Database Group
Sample_contact_institute	MRC Human Genetics Unit
Sample_contact_address	Crewe Road
Sample_contact_city	Edinburgh
Sample_contact_zip/postal_code	EH4 2XU
Sample_contact_country	United Kingdom
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM144nnn/GSM144577/suppl/GSM144577.CEL.gz
Sample_series_id	GSE6287
Sample_data_row_count	22690

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