Search results for the GEO ID: GSE6323  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM146340 | GPL339 |
|
Gastrocnemius tissue at time 0 from young mouse, biological rep1
|
gastrocnemius tissue in control-fed 5 month-old C57BL/6 mice
|
Age: 5 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 5 month-old mice on normal diet
gastrocnemius tissue at time 0 from young mouse, biological rep1
|
| Sample_geo_accession | GSM146340
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146340/suppl/GSM146340.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146341 | GPL339 |
|
Gastrocnemius tissue at time 0 from young mouse, biological rep2
|
gastrocnemius tissue in control-fed 5 month-old C57BL/6 mice
|
Age: 5 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 5 month-old mice on normal diet
gastrocnemius tissue at time 0 from young mouse, biological rep2
|
| Sample_geo_accession | GSM146341
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146341/suppl/GSM146341.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146342 | GPL339 |
|
Gastrocnemius tissue at time 0 from young mouse, biological rep3
|
gastrocnemius tissue in control-fed 5 month-old C57BL/6 mice
|
Age: 5 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 5 month-old mice on normal diet
gastrocnemius tissue at time 0 from young mouse, biological rep3
|
| Sample_geo_accession | GSM146342
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146342/suppl/GSM146342.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146343 | GPL339 |
|
Gastrocnemius tissue at time 0 from young mouse, biological rep4
|
gastrocnemius tissue in control-fed 5 month-old C57BL/6 mice
|
Age: 5 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 5 month-old mice on normal diet
gastrocnemius tissue at time 0 from young mouse, biological rep4
|
| Sample_geo_accession | GSM146343
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146343/suppl/GSM146343.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146344 | GPL339 |
|
Gastrocnemius tissue at time 0 from young mouse, biological rep5
|
gastrocnemius tissue in control-fed 5 month-old C57BL/6 mice
|
Age: 5 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 5 month-old mice on normal diet
gastrocnemius tissue at time 0 from young mouse, biological rep5
|
| Sample_geo_accession | GSM146344
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146344/suppl/GSM146344.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146345 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged mouse, biological rep1
|
gastrocnemius tissue in control-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on normal diet
gastrocnemius tissue at time 0 from aged mouse, biological rep1
|
| Sample_geo_accession | GSM146345
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146345/suppl/GSM146345.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146346 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged mouse, biological rep2
|
gastrocnemius tissue in control-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on normal diet
gastrocnemius tissue at time 0 from aged mouse, biological rep2
|
| Sample_geo_accession | GSM146346
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146346/suppl/GSM146346.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146347 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged mouse, biological rep3
|
gastrocnemius tissue in control-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on normal diet
gastrocnemius tissue at time 0 from aged mouse, biological rep3
|
| Sample_geo_accession | GSM146347
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146347/suppl/GSM146347.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146348 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged mouse, biological rep4
|
gastrocnemius tissue in control-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on normal diet
gastrocnemius tissue at time 0 from aged mouse, biological rep4
|
| Sample_geo_accession | GSM146348
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146348/suppl/GSM146348.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146349 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged mouse, biological rep5
|
gastrocnemius tissue in control-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: normal
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on normal diet
gastrocnemius tissue at time 0 from aged mouse, biological rep5
|
| Sample_geo_accession | GSM146349
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146349/suppl/GSM146349.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146350 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
|
gastrocnemius tissue in calorie restricted-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: calorie restricted
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on calorie restricted diet
gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2
|
| Sample_geo_accession | GSM146350
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146350/suppl/GSM146350.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146351 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
|
gastrocnemius tissue in calorie restricted-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: calorie restricted
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on calorie restricted diet
gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3
|
| Sample_geo_accession | GSM146351
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146351/suppl/GSM146351.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146352 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5
|
gastrocnemius tissue in calorie restricted-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: calorie restricted
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on calorie restricted diet
gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5
|
| Sample_geo_accession | GSM146352
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146352/suppl/GSM146352.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146353 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
|
gastrocnemius tissue in calorie restricted-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: calorie restricted
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on calorie restricted diet
gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6
|
| Sample_geo_accession | GSM146353
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146353/suppl/GSM146353.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
| | |
|
GSM146354 | GPL339 |
|
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
|
gastrocnemius tissue in calorie restricted-fed 25 month-old C57BL/6 mice
|
Age: 25 month-old
Diet: calorie restricted
|
Gene expression data from gastrocnemius tissue from 25 month-old mice on calorie restricted diet
gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7
|
| Sample_geo_accession | GSM146354
| | Sample_status | Public on Dec 01 2006
| | Sample_submission_date | Nov 20 2006
| | Sample_last_update_date | Nov 21 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Mus musculus
| | Sample_taxid_ch1 | 10090
| | Sample_treatment_protocol_ch1 | Mice were fed one of two life-long dietary regimens: normal diet (ND) and caloric restriction (CR). Mice on ND were provided acidic water ad libitum and fed 84 kcal/wk which is ~15% less than the average ad libitum diet. C57BL/6NHsd mice on this dietary regiment typically have an average lifespan of 30 mo. in our colony. Mice on CR were fed a diet that resulted in a 26% difference in caloric intake to the ND animals. The diet fed to CR mice was enriched in protein, vitamins and minerals such that CR and ND mice were fed nearly identical amounts of these components.
| | Sample_growth_protocol_ch1 | Male C57BL/6NHsd mice were purchased from Harlan Sprague-Dawley at 6-7 weeks of age and individually housed for the remainder of their lifespan.
| | Sample_molecule_ch1 | polyA RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions and polyadenylate [poly(A)+] RNA was purified from the total RNA with oligo-dT-linked Oligotex resin (Qiagen, Valencia, CA).
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | One microgram of poly(A)+ RNA was converted into double-stranded cDNA (ds-cDNA) by using the SuperScript Choice System (Life Technologies) with an oligo-dT primer containing a T7 RNA polymerase promoter (Genset, La Jolla, CA). The ds-cDNA was extracted with phenol-chloroform-isoamyl alcohol and precipitated with pellet paint coprecipitant (Novagen, Madison, WI). Biotin-labeled RNA was synthesized in vitro using a high-yield RNA transcript labeling kit (BioArray; Enzo, Farmingdale, NY). The biotin-labeled antisense cRNA was purified using the RNeasy affinity column (Qiagen) and fragmented randomly.
| | Sample_hyb_protocol | The hybridization cocktail (200 µl) containing 10 µg of fragmented cRNA was injected into the MOE430A array (Affymetrix, Santa Clara, CA). The GeneChip was placed in a 45°C oven at 60 rpm for 16 h. After hybridization, the GeneChips were washed and stained in a fluidic station (model 800101, Affymetrix) with signal amplification protocol using antibody.
| | Sample_scan_protocol | GeneChips were scanned at a resolution of 3 µm twice using a Hewlett-Packard GeneArray Scanner (model 900154, Affymetrix), and the averaged images were used for further analysis.
| | Sample_data_processing | Robust Multi-Array Analysis (RMA) was used to pre-process and normalize the raw Affymetrix GeneChip data. Microarray Suite version 5.0 (MAS5.0) was used to compute absent/present calls.
| | Sample_platform_id | GPL339
| | Sample_contact_name | Michael,,Edwards
| | Sample_contact_email | michael.edwards@ucdenver.edu
| | Sample_contact_phone | 303-724-6054
| | Sample_contact_fax | 303-724-6042
| | Sample_contact_department | Pulmonary
| | Sample_contact_institute | UC Denver
| | Sample_contact_address | 12700 East 19th Avenue, Box C272
| | Sample_contact_city | Aurora
| | Sample_contact_state | CO
| | Sample_contact_zip/postal_code | 80045
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM146nnn/GSM146354/suppl/GSM146354.cel.gz
| | Sample_series_id | GSE6323
| | Sample_data_row_count | 22690
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