Search results for the GEO ID: GSE6381  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM147131 | GPL96 |
|
Stage2, End-ischemia, Replicate1
|
Heart
|
End-ischemia sample was taken at 50 minutes of cardiac arrest.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147131
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD Patients
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147131/suppl/GSM147131.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147133 | GPL96 |
|
Stage2, End-ischemia, Replicate2
|
Heart
|
End-ischemia sample was taken at 50 minutes of cardiac arrest.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147133
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD Patients
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147133/suppl/GSM147133.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147134 | GPL96 |
|
Stage2, End-ischemia, Replicate3
|
Heart
|
End-ischemia sample was taken at 50 minutes of cardiac arrest.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147134
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD Patients
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147134/suppl/GSM147134.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147135 | GPL96 |
|
Stage2, End-ischemia, Replicate4
|
Heart
|
End-ischemia sample was taken at 50 minutes of cardiac arrest.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147135
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD Patients
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147135/suppl/GSM147135.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147136 | GPL96 |
|
Stage1, Immediately post-cardioplegic arrest, Replicate1
|
Heart
|
Sample was taken immediately after cardiac arrest following administration of standard hyperkalemic, cold blood cardioplegia (30ml/kg), which was repeated (20ml/kg) every 20 minutes
All myocardial samples were taken from the RVOT through the tricuspid valve
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147136
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol using MAS5.0.
| | Sample_data_processing | GeneSpring version 7.0 from Agilent.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147136/suppl/GSM147136.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147137 | GPL96 |
|
Stage1, Immediately post-cardioplegic arrest, Replicate2
|
Heart
|
Sample was taken immediately after cardiac arrest following administration of standard hyperkalemic, cold blood cardioplegia (30ml/kg), which was repeated (20ml/kg) every 20 minutes
All myocardial samples were taken from the RVOT through the tricuspid valve
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147137
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol using MAS5.0.
| | Sample_data_processing | GeneSpring version 7.0 from Agilent.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147137/suppl/GSM147137.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147138 | GPL96 |
|
Stage1, Immediately post-cardioplegic arrest, Replicate3
|
Heart
|
Sample was taken immediately after cardiac arrest following administration of standard hyperkalemic, cold blood cardioplegia (30ml/kg), which was repeated (20ml/kg) every 20 minutes
All myocardial samples were taken from the RVOT through the tricuspid valve
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147138
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol using MAS5.0.
| | Sample_data_processing | GeneSpring version 7.0 from Agilent.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147138/suppl/GSM147138.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM147139 | GPL96 |
|
Stage1, Immediately post-cardioplegic arrest, Replicate4
|
Heart
|
Sample was taken immediately after cardiac arrest following administration of standard hyperkalemic, cold blood cardioplegia (30ml/kg), which was repeated (20ml/kg) every 20 minutes
All myocardial samples were taken from the RVOT through the tricuspid valve
|
All the chips were controlled for presence of any defect before scanning.
|
| Sample_geo_accession | GSM147139
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Nov 27 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | Affymetrix standard protocol using MAS5.0.
| | Sample_data_processing | GeneSpring version 7.0 from Agilent.
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147139/suppl/GSM147139.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM148145 | GPL96 |
|
Stage 3, after 5 min. Reperfusion, Replicate1
|
Heart
|
Sample was taken at 5 minutes after reperfusion prior to right atrial closure.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning
|
| Sample_geo_accession | GSM148145
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Dec 04 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD patient
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | MAS5.0 Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM148nnn/GSM148145/suppl/GSM148145.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM148146 | GPL96 |
|
Stage 3, after 5 min. Reperfusion, Replicate2
|
Heart
|
Sample was taken at 5 minutes after reperfusion prior to right atrial closure.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning
|
| Sample_geo_accession | GSM148146
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Dec 04 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD patient
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | MAS5.0 Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM148nnn/GSM148146/suppl/GSM148146.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM148147 | GPL96 |
|
Stage 3, after 5 min. Reperfusion, Replicate3
|
Heart
|
Sample was taken at 5 minutes after reperfusion prior to right atrial closure.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning
|
| Sample_geo_accession | GSM148147
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Dec 04 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD patient
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | MAS5.0 Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM148nnn/GSM148147/suppl/GSM148147.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
| | |
|
GSM148149 | GPL96 |
|
Stage 3, after 5 min. Reperfusion, Replicate4
|
Heart
|
Sample was taken at 5 minutes after reperfusion prior to right atrial closure.
All myocardial samples were taken from the RVOT through the tricuspid valve.
|
All the chips were controlled for presence of any defect before scanning
|
| Sample_geo_accession | GSM148149
| | Sample_status | Public on Nov 19 2007
| | Sample_submission_date | Dec 04 2006
| | Sample_last_update_date | Nov 19 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | VSD patient
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA (TRNA) was isolated utilizing Trizol Reagent (GIBCO/BRL) following the manufacturer's protocol. The quality of TRNA was assessed using the Agilent 2100 Bioanalyzer (version A.02.01S1232, Agilent Technologies). Only RNA with the OD ratio of 1.99-2.0 at 260/280 was used for microarray analysis.
| | Sample_label_ch1 | Biotin
| | Sample_label_protocol_ch1 | Labeling was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_label_protocol_ch1 | Affymetrix standard protocol
| | Sample_hyb_protocol | Hybridization was performed according to standard Affymetrix instructions at the Genomic Core Facility at the Hospital for Sick Children.
| | Sample_hyb_protocol | Affymetrix standard protocol
| | Sample_scan_protocol | MAS5.0 Affymetrix standard protocol
| | Sample_data_processing | GeneSpring version 7.0 from Agilent
| | Sample_platform_id | GPL96
| | Sample_contact_name | Sara,,Arab
| | Sample_contact_laboratory | John Coles
| | Sample_contact_department | Cardiology
| | Sample_contact_institute | Hospital for Sick Children
| | Sample_contact_address | 555 University Ave
| | Sample_contact_city | Toronto
| | Sample_contact_state | ONT
| | Sample_contact_zip/postal_code | M5G 1X8
| | Sample_contact_country | Canada
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM148nnn/GSM148149/suppl/GSM148149.CEL.gz
| | Sample_series_id | GSE6381
| | Sample_data_row_count | 22283
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