Search results for the GEO ID: GSE6400 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM147099 | GPL570 |
|
Actinomycin D treated A549 human lung cancer cell cultures replicate A
|
Actinomycin D treated A549 human lung cancer cell cultures replicate A
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147099
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 25 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147099/suppl/GSM147099.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147363 | GPL570 |
|
Actinomycin D treated A549 human lung cancer cell cultures replicate B
|
Actinomycin D treated A549 human lung cancer cell cultures replicate B
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147363
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147363/suppl/GSM147363.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147364 | GPL570 |
|
Actinomycin D treated A549 human lung cancer cell cultures replicate C
|
Actinomycin D treated A549 human lung cancer cell cultures replicate C
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147364
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, actinomycin D (5 ug/mL final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147364/suppl/GSM147364.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147365 | GPL570 |
|
Mannitol control treated A549 human lung cancer cell cultures replicate A
|
Mannitol control treated A549 human lung cancer cell cultures replicate A
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147365
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147365/suppl/GSM147365.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147366 | GPL570 |
|
Mannitol control treated A549 human lung cancer cell cultures replicate B
|
Mannitol control treated A549 human lung cancer cell cultures replicate B
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147366
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147366/suppl/GSM147366.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147367 | GPL570 |
|
Mannitol control treated A549 human lung cancer cell cultures replicate C
|
Mannitol control treated A549 human lung cancer cell cultures replicate C
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147367
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, mannitol (5% final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147367/suppl/GSM147367.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147368 | GPL570 |
|
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A
|
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147368
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147368/suppl/GSM147368.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147369 | GPL570 |
|
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B
|
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147369
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147369/suppl/GSM147369.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
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GSM147370 | GPL570 |
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1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C
|
1.25uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147370
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 28 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 1.25uM sapphyrin PCI-2050 (1.25uM final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147370/suppl/GSM147370.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147520 | GPL570 |
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate A
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147520
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 29 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147520/suppl/GSM147520.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147521 | GPL570 |
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate B
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147521
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 29 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147521/suppl/GSM147521.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
|
GSM147522 | GPL570 |
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C
|
2.5uM sapphyrin PCI-2050 treated A549 human lung cancer cell cultures replicate C
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
Sample_geo_accession | GSM147522
| Sample_status | Public on Dec 01 2006
| Sample_submission_date | Nov 29 2006
| Sample_last_update_date | Nov 30 2006
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_biomaterial_provider_ch1 | ATCC
| Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_growth_protocol_ch1 | A549 human lung cancer cells (1X10^5 per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded eights days prior to treatment of non-cycling plateau phase cultures with drug. At four hours prior to RNA isolation, 2.5uM sapphyrin PCI-2050 (final concentration) was added to the culture. After incubation for four hours, the culture was washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recommended protocols.
| Sample_label_ch1 | Affymetrix single-stage biotin
| Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recommended protocols.
| Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recommended protocols.
| Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recommended protocols.
| Sample_data_processing | RMA Normalization
| Sample_platform_id | GPL570
| Sample_contact_name | Joseph,Gerard,Hacia
| Sample_contact_email | hacia@hsc.usc.edu
| Sample_contact_phone | 323-442-3030
| Sample_contact_fax | 323-442-2764
| Sample_contact_laboratory | Hacia Lab
| Sample_contact_department | Biochemistry and Molecular Biology
| Sample_contact_institute | University of Southern California
| Sample_contact_address | 2250 Alcazar Street, IGM 240
| Sample_contact_city | Los Angeles
| Sample_contact_state | CA
| Sample_contact_zip/postal_code | 90089
| Sample_contact_country | USA
| Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM147nnn/GSM147522/suppl/GSM147522.CEL.gz
| Sample_series_id | GSE6400
| Sample_data_row_count | 54675
| |
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