Search results for the GEO ID: GSE6521  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM149913 | GPL570 |
|
MCF7_inhibitor_control_for_U0126
|
breast epithelial cell, adherent, without stimulus, control for U0126
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation.
|
| Sample_geo_accession | GSM149913
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149913/suppl/GSM149913.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149913/suppl/GSM149913.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149914 | GPL570 |
|
MCF7_inhibitor_control_for_AG1478
|
breast epithelial cell, adherent, without stimulus, control for AG1478
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation.
|
| Sample_geo_accession | GSM149914
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149914/suppl/GSM149914.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149914/suppl/GSM149914.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149915 | GPL570 |
|
MCF7_inhibitor_HRG_5min
|
breast epithelial cell, adherent, HRG, 5min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149915
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149915/suppl/GSM149915.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149915/suppl/GSM149915.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149916 | GPL570 |
|
MCF7_inhibitor_HRG_10min
|
breast epithelial cell, adherent, HRG, 10min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149916
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149916/suppl/GSM149916.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149916/suppl/GSM149916.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149917 | GPL570 |
|
MCF7_inhibitor_HRG_15min
|
breast epithelial cell, adherent, HRG, 15min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149917
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149917/suppl/GSM149917.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149917/suppl/GSM149917.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149918 | GPL570 |
|
MCF7_inhibitor_HRG_30min
|
breast epithelial cell, adherent, HRG, 30min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149918
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149918/suppl/GSM149918.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149918/suppl/GSM149918.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149919 | GPL570 |
|
MCF7_inhibitor_HRG_45min
|
breast epithelial cell, adherent, HRG, 45min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149919
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149919/suppl/GSM149919.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149919/suppl/GSM149919.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149920 | GPL570 |
|
MCF7_inhibitor_HRG_60min
|
breast epithelial cell, adherent, HRG, 60min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149920
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149920/suppl/GSM149920.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149920/suppl/GSM149920.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149921 | GPL570 |
|
MCF7_inhibitor_HRG_90min
|
breast epithelial cell, adherent, HRG, 90min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation
|
| Sample_geo_accession | GSM149921
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149921/suppl/GSM149921.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149921/suppl/GSM149921.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149922 | GPL570 |
|
MCF7_inhibitor_U0126_5min
|
breast epithelial cell, adherent, U0126, 5min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149922
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149922/suppl/GSM149922.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149922/suppl/GSM149922.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149923 | GPL570 |
|
MCF7_inhibitor_U0126_10min
|
breast epithelial cell, adherent, U0126, 10min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149923
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149923/suppl/GSM149923.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149923/suppl/GSM149923.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149924 | GPL570 |
|
MCF7_inhibitor_U0126_15min
|
breast epithelial cell, adherent, U0126, 15min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149924
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149924/suppl/GSM149924.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149924/suppl/GSM149924.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149925 | GPL570 |
|
MCF7_inhibitor_U0126_30min
|
breast epithelial cell, adherent, U0126, 30min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149925
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149925/suppl/GSM149925.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149925/suppl/GSM149925.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149926 | GPL570 |
|
MCF7_inhibitor_U0126_45min
|
breast epithelial cell, adherent, U0126, 45min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149926
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149926/suppl/GSM149926.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149926/suppl/GSM149926.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149927 | GPL570 |
|
MCF7_inhibitor_U0126_60min
|
breast epithelial cell, adherent, U0126, 60min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149927
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149927/suppl/GSM149927.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149927/suppl/GSM149927.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149928 | GPL570 |
|
MCF7_inhibitor_U0126_90min
|
breast epithelial cell, adherent, U0126, 90min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by a MEK inhibitor stimulation.
|
| Sample_geo_accession | GSM149928
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149928/suppl/GSM149928.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149928/suppl/GSM149928.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149929 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_5min
|
breast epithelial cell, adherent, HRG, U0126, 5min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149929
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149929/suppl/GSM149929.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149929/suppl/GSM149929.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149930 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_10min
|
breast epithelial cell, adherent, HRG, U0126, 10min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149930
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149930/suppl/GSM149930.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149930/suppl/GSM149930.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149931 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_15min
|
breast epithelial cell, adherent, HRG, U0126, 15min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149931
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149931/suppl/GSM149931.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149931/suppl/GSM149931.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149932 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_30min
|
breast epithelial cell, adherent, HRG, U0126, 30min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149932
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149932/suppl/GSM149932.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149932/suppl/GSM149932.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149933 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_45min
|
breast epithelial cell, adherent, HRG, U0126, 45min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149933
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149933/suppl/GSM149933.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149933/suppl/GSM149933.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149934 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_60min
|
breast epithelial cell, adherent, HRG, U0126, 60min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149934
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149934/suppl/GSM149934.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149934/suppl/GSM149934.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149935 | GPL570 |
|
MCF7_inhibitor_HRG_U0126_90min
|
breast epithelial cell, adherent, HRG, U0126, 90min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with a MEK inhibitor.
|
| Sample_geo_accession | GSM149935
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, U0126 (a MEK inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149935/suppl/GSM149935.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149935/suppl/GSM149935.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149936 | GPL570 |
|
MCF7_inhibitor_AG1478_5min
|
breast epithelial cell, adherent, AG1478, 5min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149936
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149936/suppl/GSM149936.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149936/suppl/GSM149936.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149937 | GPL570 |
|
MCF7_inhibitor_AG1478_10min
|
breast epithelial cell, adherent, AG1478, 10min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149937
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149937/suppl/GSM149937.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149937/suppl/GSM149937.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149938 | GPL570 |
|
MCF7_inhibitor_AG1478_15min
|
breast epithelial cell, adherent, AG1478, 15min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149938
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149938/suppl/GSM149938.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149938/suppl/GSM149938.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149939 | GPL570 |
|
MCF7_inhibitor_AG1478_30min
|
breast epithelial cell, adherent, AG1478, 30min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149939
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149939/suppl/GSM149939.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149939/suppl/GSM149939.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149940 | GPL570 |
|
MCF7_inhibitor_AG1478_45min
|
breast epithelial cell, adherent, AG1478, 45min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149940
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149940/suppl/GSM149940.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149940/suppl/GSM149940.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149941 | GPL570 |
|
MCF7_inhibitor_AG1478_60min
|
breast epithelial cell, adherent, AG1478, 60min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149941
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149941/suppl/GSM149941.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149941/suppl/GSM149941.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149942 | GPL570 |
|
MCF7_inhibitor_AG1478_90min
|
breast epithelial cell, adherent, AG1478, 90min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by an EGFR kinase inhibitor stimulation.
|
| Sample_geo_accession | GSM149942
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the inhibitor treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the inhibitor treatment
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149942/suppl/GSM149942.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149942/suppl/GSM149942.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149943 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_5min
|
breast epithelial cell, adherent, HRG, AG1478, 5min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149943
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149943/suppl/GSM149943.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149943/suppl/GSM149943.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149944 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_10min
|
breast epithelial cell, adherent, HRG, AG1478, 10min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149944
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149944/suppl/GSM149944.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149944/suppl/GSM149944.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149945 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_15min
|
breast epithelial cell, adherent, HRG, AG1478, 15min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149945
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149945/suppl/GSM149945.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149945/suppl/GSM149945.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149947 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_45min
|
breast epithelial cell, adherent, HRG, AG1478, 45min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149947
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149947/suppl/GSM149947.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149947/suppl/GSM149947.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149948 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_60min
|
breast epithelial cell, adherent, HRG, AG1478, 60min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149948
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149948/suppl/GSM149948.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149948/suppl/GSM149948.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
GSM149949 | GPL570 |
|
MCF7_inhibitor_HRG_AG1478_90min
|
breast epithelial cell, adherent, HRG, AG1478, 90min
|
Cell line: MCF-7 (Obtained from ATCC (American Type Culture Collection)), Organ: mammary gland; breast, Cell type: epithelial, Disease: adenocarcinoma, Derived from metastatic site: pleural effusion, Age: 69 years adult, Gender: female, Ethnicity: Caucasian
|
Early gene expression (up to 90min) induced by growth hormone stimulation with an EGFR kinase inhibitor.
|
| Sample_geo_accession | GSM149949
| | Sample_status | Public on Dec 15 2006
| | Sample_submission_date | Dec 13 2006
| | Sample_last_update_date | Dec 14 2006
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Before treatments, medium were changed with serum-free medium again and incubated at 37C for 2 hours. Followed by incubation, added each ligand and incubated for each time.
| | Sample_growth_protocol_ch1 | Prior to the growth factor (GF) treatment, cells were serum starved for 16-18 hours with serum-free DMEM medium and incubated at 37C/5% CO2. The medium was again changed 2 hr prior to the GF treatment. The inhibitor, AG1478 (an EGFR kinase inhibitor) was added to 20 min prior to the GF treatment.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was isolated using TRIzol reagent (Invitrogen) and then purified using QIAGEN Rneasy kit.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, chapter1-p13-18, & 2.1.p32-33,p36-40)
| | Sample_hyb_protocol | Standard Affymetrix Protocols (Affymetrix GeneChip Expression Analysis Technical Manual) was used. Complete protocal can be found manufacturer's web site (http://www.affymetrix.com/support/technical/manual/expression_manual.affx) (section2, 2.2.p3-8 & 2.3.p3-14)
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000 with default parameter.
| | Sample_data_processing | The data were analyzed with GeneChip Operating System (GCOS) (ver.1.2) using Affymetrix default analysis settings and global scaling as normalization method. The Single-Array Expression Analysis function was used and the trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Mariko,,Okada
| | Sample_contact_email | marikoh@rcai.riken.jp
| | Sample_contact_laboratory | Laboratory for Cellular Systems Modeling
| | Sample_contact_institute | RIKEN RCAI
| | Sample_contact_address | W518, 1-7-22, Suehiro-cho, Tsurumi-ku
| | Sample_contact_city | Yokohama
| | Sample_contact_zip/postal_code | 230-0045
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149949/suppl/GSM149949.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM149nnn/GSM149949/suppl/GSM149949.EXP.gz
| | Sample_series_id | GSE6521
| | Sample_data_row_count | 54675
| | |
|
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(2 groups will be compared at a time) |
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