Search results for the GEO ID: GSE6541 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM151566 | GPL341 |
|
60272_2SALINE
|
Rattus norvegicus heart, intratracheal saline
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal saline replicate #2
|
Sample_geo_accession | GSM151566
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151566/suppl/GSM151566.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151567 | GPL341 |
|
60273_3SALINE
|
Rattus norvegicus heart, intratracheal saline
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal saline replicate #3
|
Sample_geo_accession | GSM151567
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151567/suppl/GSM151567.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151568 | GPL341 |
|
60274_4SALINE
|
Rattus norvegicus heart, intratracheal saline
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal saline, replicate #4
|
Sample_geo_accession | GSM151568
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151568/suppl/GSM151568.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151569 | GPL341 |
|
60469_1SALINE-2
|
Rattus norvegicus heart, intratracheal saline
|
Healthy, male 12-14 week old, Wistar Kyoto rats
|
Intratracheal saline, replicate #1
|
Sample_geo_accession | GSM151569
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151569/suppl/GSM151569.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151570 | GPL341 |
|
60275_5MSH
|
Rattus norvegicus heart, intratracheal Mt. St. Helen ash
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal Mount St. Helen's (MSH) ash which does not contain any water-soluble zinc or other metals such that we can delineate any cardiac effect secondary to pulmonary deposition of these particles as these fine mode particles themselves are not likely to translocate to the heart, replicate #1
|
Sample_geo_accession | GSM151570
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151570/suppl/GSM151570.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151571 | GPL341 |
|
60276_6MSH
|
Rattus norvegicus heart, intratracheal Mt. St. Helen ash
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal Mount St. Helen's (MSH) ash which does not contain any water-soluble zinc or other metals such that we can delineate any cardiac effect secondary to pulmonary deposition of these particles as these fine mode particles themselves are not likely to translocate to the heart, replicate #2
|
Sample_geo_accession | GSM151571
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151571/suppl/GSM151571.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151572 | GPL341 |
|
60277_7MSH
|
Rattus norvegicus heart, intratracheal Mt. St. Helen ash
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal Mount St. Helen's (MSH) ash which does not contain any water-soluble zinc or other metals such that we can delineate any cardiac effect secondary to pulmonary deposition of these particles as these fine mode particles themselves are not likely to translocate to the heart, replicate #3
|
Sample_geo_accession | GSM151572
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151572/suppl/GSM151572.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151573 | GPL341 |
|
60278_8MSH
|
Rattus norvegicus heart, intratracheal Mt. St. Helen ash
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Intratracheal Mount St. Helen's (MSH) ash which does not contain any water-soluble zinc or other metals such that we can delineate any cardiac effect secondary to pulmonary deposition of these particles as these fine mode particles themselves are not likely to translocate to the heart, replicate #4
|
Sample_geo_accession | GSM151573
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151573/suppl/GSM151573.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151598 | GPL341 |
|
60279_9HP11HD
|
Rattus norvegicus heart, intratracheal 14.5 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (14.5ug/mg zinc) and also a small amount of water-soluble nickel (3.0 ug/mg), replicate #1
|
Sample_geo_accession | GSM151598
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151598/suppl/GSM151598.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151599 | GPL341 |
|
60280_10HP11HD
|
Rattus norvegicus heart, intratracheal 14.5 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (14.5ug/mg zinc) and also a small amount of water-soluble nickel (3.0 ug/mg), replicate #2.
|
Sample_geo_accession | GSM151599
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151599/suppl/GSM151599.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151600 | GPL341 |
|
60281_11HP11HD
|
Rattus norvegicus heart, intratracheal 14.5 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (14.5ug/mg zinc) and also a small amount of water-soluble nickel (3.0 ug/mg), replicate #3.
|
Sample_geo_accession | GSM151600
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151600/suppl/GSM151600.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151601 | GPL341 |
|
60282_12HP11HD
|
Rattus norvegicus heart, intratracheal 14.5 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (14.5ug/mg zinc) and also a small amount of water-soluble nickel (3.0 ug/mg), replicate #4.
|
Sample_geo_accession | GSM151601
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151601/suppl/GSM151601.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151605 | GPL341 |
|
60283_13HP11LD
|
Rattus norvegicus heart, intratracheal 7.25 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (7.25ug/mg zinc) and also a small amount of water-soluble nickel (1.5 ug/mg), replicate #1.
|
Sample_geo_accession | GSM151605
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151605/suppl/GSM151605.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151609 | GPL341 |
|
60284_14HP11LD
|
Rattus norvegicus heart, intratracheal 7.25 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (7.25ug/mg zinc) and also a small amount of water-soluble nickel (1.5 ug/mg), replicate #2.
|
Sample_geo_accession | GSM151609
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151609/suppl/GSM151609.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151610 | GPL341 |
|
60285_15HP11LD
|
Rattus norvegicus heart, intratracheal 7.25 ug/mg zinc
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained insoluble components plus water soluble zinc (7.25ug/mg zinc) and also a small amount of water-soluble nickel (1.5 ug/mg), replicate #3.
|
Sample_geo_accession | GSM151610
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151610/suppl/GSM151610.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151611 | GPL341 |
|
60286_16HP11LD
|
Rattus norvegicus heart, intratracheal 7.25 ug/mg zinc.
|
Healthy, male, 12-14 week old, Wistar Kyoto rats.
|
Saline suspension which contained insoluble components plus water soluble zinc (7.25ug/mg zinc) and also a small amount of water-soluble nickel (1.5 ug/mg), replicate #4.
|
Sample_geo_accession | GSM151611
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151611/suppl/GSM151611.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151613 | GPL341 |
|
60287_17HP11LC
|
Rattus norvegicus heart, intratracheal zinc leachate
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained soluble zinc leachate components, replicate #1.
|
Sample_geo_accession | GSM151613
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151613/suppl/GSM151613.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151614 | GPL341 |
|
60288_18HP11LC
|
Rattus norvegicus heart, intratracheal zinc leachate
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained soluble zinc leachate components, replicate #2.
|
Sample_geo_accession | GSM151614
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151614/suppl/GSM151614.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151615 | GPL341 |
|
60289_19HP11LC
|
Rattus norvegicus heart, intratracheal zinc leachate
|
Healthy, male, 12-14 week old, Wistar Kyoto rats.
|
Saline suspension which contained soluble zinc leachate components, replicate #3.
|
Sample_geo_accession | GSM151615
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151615/suppl/GSM151615.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151616 | GPL341 |
|
60290_20HP11LC
|
Rattus norvegicus heart, intratracheal zinc leachate
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained soluble zinc leachate components, replicate #4.
|
Sample_geo_accession | GSM151616
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151616/suppl/GSM151616.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
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GSM151617 | GPL341 |
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60291_21ZN
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Rattus norvegicus heart, intratracheal zinc sulfate
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Healthy, male, 12-14 week old, Wistar Kyoto rats
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Saline suspension which contained zinc sulfate (14.5ug/ml), replicate #1.
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Sample_geo_accession | GSM151617
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151617/suppl/GSM151617.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
| |
|
GSM151618 | GPL341 |
|
60292_22ZN
|
Rattus norvegicus heart, intratracheal zinc sulfate
|
Healthy, male, 12-14 week old, Wistar Kyoto rats
|
Saline suspension which contained zinc sulfate (14.5ug/ml), replicate #2.
|
Sample_geo_accession | GSM151618
| Sample_status | Public on Nov 15 2007
| Sample_submission_date | Dec 15 2006
| Sample_last_update_date | Nov 15 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_biomaterial_provider_ch1 | Charles River Laboratories, Raleigh, NC
| Sample_treatment_protocol_ch1 | Rats in each group were randomized by body weight into 6 groups (n=8) for each time point. Each particle sample was suspended in sterile saline at desired concentration and was mixed continuously for 20 min prior to their use. To prepare soluble fraction, PM saline suspension was centrifuged at 12, 000 x g in cold for 10 min and the supernatant fluid was filtered through a Teflon syringe filter unit (0.25 um pore size). This filtered fraction was termed as leachable fraction (PM-L). Similarly zinc sulfate was dissolved in sterile saline to give 33.4 ug zinc per ml. All fractions, including saline were intratracheally instilled once per week at 1 ml/kg volume under halothane anesthesia
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total heart RNA was isolated from tissues snap-frozen in liquid nitrogen by homogenization in TriReagent (Sigma, St Louis, MO) and dissolved in 50ul diethylpyrocarbonate treated water.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | see Affymetrix technical manual
| Sample_hyb_protocol | see Affymetrix technical manual
| Sample_scan_protocol | see Affymetrix technical manual
| Sample_data_processing | MAS 5.0
| Sample_platform_id | GPL341
| Sample_contact_name | Urmila,P,Kodavanti
| Sample_contact_email | kodavanti.urmila@epa.gov
| Sample_contact_phone | 919-541-4963
| Sample_contact_fax | 919-541-0026
| Sample_contact_laboratory | B143-01
| Sample_contact_department | NHEERL, ETD
| Sample_contact_institute | USEPA
| Sample_contact_address | 109 TW Alexander Drive
| Sample_contact_city | Research Triangle Park
| Sample_contact_state | NC
| Sample_contact_zip/postal_code | 27711
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM151nnn/GSM151618/suppl/GSM151618.CEL.gz
| Sample_series_id | GSE6541
| Sample_data_row_count | 15923
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