Search results for the GEO ID: GSE6866 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM154159 | GPL339 |
|
soshin WT-3
|
WT-3 hair follicle, postnatal day 14
|
Strain: N1 offspring (+/Hr) between +/Hr and Hr/Hr
Gender: Male
Tissue: Hair follicles of dorsal skin, non-treatment
|
All required informations are described in other fields.
|
Sample_geo_accession | GSM154159
| Sample_status | Public on Jul 11 2008
| Sample_submission_date | Jan 05 2007
| Sample_last_update_date | Jul 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | polyA RNA
| Sample_extract_protocol_ch1 | Fresh skin samples were embedded in Tissue-Tek O.C.T. Compound (SAKURA Finetechnical Co., Ltd. Tokyo, Japan) and kept at -80 ℃ until used. Tissues were sectioned using a cryostat at 8-μm thickness and placed on Membrane Slides (Leica Microsystems Inc., Tokyo, Japan). The sections were fixed in methanol for 30 sec, rinsed in water, stained with HistoGeneTM Staining Solution (Takara Shuzo, Ohtsu, Japan) for 30 sec, and dehydrated by sequential exposure to 75 %, 95 %, and 100 % ethanol, and xylene.
| Sample_extract_protocol_ch1 | Sections were air-dried and used for LCM of hair follicles with an Application Solutions Laser Micrdissection System (Leica Microsystems Inc., Tokyo, Japan) Fifty to seventy hair follicles per sample were collected in Buffer RLT (QIAGEN) and preserved at -80 ℃ until RNA extraction. RNA was extracted with an RNeasy Micro Kit (QIAGEN). The quality of RNA in samples was checked by Agilent 2100 Bioanalyzer (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was prepared from 1 μg of aRNA using the SuperScript Double Strand cDNA Synthesis Kit (Invitrogen). T7-(dT)24 primer (primer sequence: 5’GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(T)24-3’), was used for first strand cDNA synthesis (Amersham Biosciences, Tokyo, Japan). Then, biotinylated cRNA was synthesized employing the GeneChip Expression Reagents for IVT Labeling Kit (Affymetrix, USA).
| Sample_hyb_protocol | After 20 μg of biotin-labeled cRNA was fragmented, hybridization solution was prepared using a GeneChip Eukaryotic Hybridization Control Kit (Affymetrix) and was hybridized to the Affymetrix Mouse Genome MOE430B Array for 45 ℃ for 16 hrs in GeneChip Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | For microarray imaging data analysis, the proprietary software (GeneChip Microarray Suite, v. 5.0, Affymetrix) was used. In brief, total array normalization was performed comparing the data between hr/hr and +/hr, and normalized data were expressed as a fold change (log 10 ratio).
| Sample_data_processing | Normalization was performed in accordance with mouse maintenance genes on the Mouse Set 430A (Affymetrix).
| Sample_platform_id | GPL339
| Sample_contact_name | Tomomi,,Soshin
| Sample_contact_email | soushintmm@chugai-pharm.co.jp
| Sample_contact_phone | +81-3-5841-5401
| Sample_contact_fax | +81-3-5841-8185
| Sample_contact_laboratory | Veterinary Pathology
| Sample_contact_department | Graduate School of Agricultural and Life Sciences
| Sample_contact_institute | the University of Tokyo
| Sample_contact_address | Yayoi 1-1-1
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-8657
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM154nnn/GSM154159/suppl/GSM154159.CEL.gz
| Sample_series_id | GSE6866
| Sample_data_row_count | 22690
| |
|
GSM154160 | GPL339 |
|
soshin WT-4
|
WT-4 hair follicle, postnatal day 14
|
Strain: N1 offspring (+/Hr) between +/Hr and Hr/Hr
Gender: Male
Tissue: Hair follicles of dorsal skin, non-treatment
|
All required informations are described in other fields.
|
Sample_geo_accession | GSM154160
| Sample_status | Public on Jul 11 2008
| Sample_submission_date | Jan 05 2007
| Sample_last_update_date | Jul 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | polyA RNA
| Sample_extract_protocol_ch1 | Fresh skin samples were embedded in Tissue-Tek O.C.T. Compound (SAKURA Finetechnical Co., Ltd. Tokyo, Japan) and kept at -80 ℃ until used. Tissues were sectioned using a cryostat at 8-μm thickness and placed on Membrane Slides (Leica Microsystems Inc., Tokyo, Japan). The sections were fixed in methanol for 30 sec, rinsed in water, stained with HistoGeneTM Staining Solution (Takara Shuzo, Ohtsu, Japan) for 30 sec, and dehydrated by sequential exposure to 75 %, 95 %, and 100 % ethanol, and xylene.
| Sample_extract_protocol_ch1 | Sections were air-dried and used for LCM of hair follicles with an Application Solutions Laser Micrdissection System (Leica Microsystems Inc., Tokyo, Japan) Fifty to seventy hair follicles per sample were collected in Buffer RLT (QIAGEN) and preserved at -80 ℃ until RNA extraction. RNA was extracted with an RNeasy Micro Kit (QIAGEN). The quality of RNA in samples was checked by Agilent 2100 Bioanalyzer (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was prepared from 1 μg of aRNA using the SuperScript Double Strand cDNA Synthesis Kit (Invitrogen). T7-(dT)24 primer (primer sequence: 5’GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(T)24-3’), was used for first strand cDNA synthesis (Amersham Biosciences, Tokyo, Japan). Then, biotinylated cRNA was synthesized employing the GeneChip Expression Reagents for IVT Labeling Kit (Affymetrix, USA).
| Sample_hyb_protocol | After 20 μg of biotin-labeled cRNA was fragmented, hybridization solution was prepared using a GeneChip Eukaryotic Hybridization Control Kit (Affymetrix) and was hybridized to the Affymetrix Mouse Genome MOE430B Array for 45 ℃ for 16 hrs in GeneChip Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | For microarray imaging data analysis, the proprietary software (GeneChip Microarray Suite, v. 5.0, Affymetrix) was used. In brief, total array normalization was performed comparing the data between hr/hr and +/hr, and normalized data were expressed as a fold change (log 10 ratio).
| Sample_data_processing | Normalization was performed in accordance with mouse maintenance genes on the Mouse Set 430A (Affymetrix).
| Sample_platform_id | GPL339
| Sample_contact_name | Tomomi,,Soshin
| Sample_contact_email | soushintmm@chugai-pharm.co.jp
| Sample_contact_phone | +81-3-5841-5401
| Sample_contact_fax | +81-3-5841-8185
| Sample_contact_laboratory | Veterinary Pathology
| Sample_contact_department | Graduate School of Agricultural and Life Sciences
| Sample_contact_institute | the University of Tokyo
| Sample_contact_address | Yayoi 1-1-1
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-8657
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM154nnn/GSM154160/suppl/GSM154160.CEL.gz
| Sample_series_id | GSE6866
| Sample_data_row_count | 22690
| |
|
GSM154161 | GPL339 |
|
soshin HR-2
|
HR-2 hair follicle, postnatal day 14
|
Strain: N1 offspring (Hr/Hr) between +/Hr and Hr/Hr
Gender: Male
Tissue: Hair follicles of dorsal skin, non-treatment
|
All required informations are described in other fields.
|
Sample_geo_accession | GSM154161
| Sample_status | Public on Jul 11 2008
| Sample_submission_date | Jan 05 2007
| Sample_last_update_date | Jul 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | polyA RNA
| Sample_extract_protocol_ch1 | Fresh skin samples were embedded in Tissue-Tek O.C.T. Compound (SAKURA Finetechnical Co., Ltd. Tokyo, Japan) and kept at -80 ℃ until used. Tissues were sectioned using a cryostat at 8-μm thickness and placed on Membrane Slides (Leica Microsystems Inc., Tokyo, Japan). The sections were fixed in methanol for 30 sec, rinsed in water, stained with HistoGeneTM Staining Solution (Takara Shuzo, Ohtsu, Japan) for 30 sec, and dehydrated by sequential exposure to 75 %, 95 %, and 100 % ethanol, and xylene.
| Sample_extract_protocol_ch1 | Sections were air-dried and used for LCM of hair follicles with an Application Solutions Laser Micrdissection System (Leica Microsystems Inc., Tokyo, Japan) Fifty to seventy hair follicles per sample were collected in Buffer RLT (QIAGEN) and preserved at -80 ℃ until RNA extraction. RNA was extracted with an RNeasy Micro Kit (QIAGEN). The quality of RNA in samples was checked by Agilent 2100 Bioanalyzer (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was prepared from 1 μg of aRNA using the SuperScript Double Strand cDNA Synthesis Kit (Invitrogen). T7-(dT)24 primer (primer sequence: 5’GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(T)24-3’), was used for first strand cDNA synthesis (Amersham Biosciences, Tokyo, Japan). Then, biotinylated cRNA was synthesized employing the GeneChip Expression Reagents for IVT Labeling Kit (Affymetrix, USA).
| Sample_hyb_protocol | After 20 microgram of biotin-labeled cRNA was fragmented, hybridization solution was prepared using a GeneChip Eukaryotic Hybridization Control Kit (Affymetrix) and was hybridized to the Affymetrix Mouse Genome MOE430B Array for 45 ℃ for 16 hrs in GeneChip Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | For microarray imaging data analysis, the proprietary software (GeneChip Microarray Suite, v. 5.0, Affymetrix) was used. In brief, total array normalization was performed comparing the data between hr/hr and +/hr, and normalized data were expressed as a fold change (log 10 ratio).
| Sample_data_processing | Normalization was performed in accordance with mouse maintenance genes on the Mouse Set 430A (Affymetrix).
| Sample_platform_id | GPL339
| Sample_contact_name | Tomomi,,Soshin
| Sample_contact_email | soushintmm@chugai-pharm.co.jp
| Sample_contact_phone | +81-3-5841-5401
| Sample_contact_fax | +81-3-5841-8185
| Sample_contact_laboratory | Veterinary Pathology
| Sample_contact_department | Graduate School of Agricultural and Life Sciences
| Sample_contact_institute | the University of Tokyo
| Sample_contact_address | Yayoi 1-1-1
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-8657
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM154nnn/GSM154161/suppl/GSM154161.CEL.gz
| Sample_series_id | GSE6866
| Sample_data_row_count | 22690
| |
|
GSM154162 | GPL339 |
|
soshin HR-3
|
HR-2 hair follicle, postnatal day 14
|
Strain: N1 offspring (+/Hr) between +/Hr and Hr/Hr
Gender: Male
Tissue: Hair follicles of dorsal skin, non-treatment
|
All required informations are described in other fields.
|
Sample_geo_accession | GSM154162
| Sample_status | Public on Jul 11 2008
| Sample_submission_date | Jan 05 2007
| Sample_last_update_date | Jul 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_molecule_ch1 | polyA RNA
| Sample_extract_protocol_ch1 | Fresh skin samples were embedded in Tissue-Tek O.C.T. Compound (SAKURA Finetechnical Co., Ltd. Tokyo, Japan) and kept at -80 ℃ until used. Tissues were sectioned using a cryostat at 8-μm thickness and placed on Membrane Slides (Leica Microsystems Inc., Tokyo, Japan). The sections were fixed in methanol for 30 sec, rinsed in water, stained with HistoGeneTM Staining Solution (Takara Shuzo, Ohtsu, Japan) for 30 sec, and dehydrated by sequential exposure to 75 %, 95 %, and 100 % ethanol, and xylene.
| Sample_extract_protocol_ch1 | Sections were air-dried and used for LCM of hair follicles with an Application Solutions Laser Micrdissection System (Leica Microsystems Inc., Tokyo, Japan) Fifty to seventy hair follicles per sample were collected in Buffer RLT (QIAGEN) and preserved at -80 ℃ until RNA extraction. RNA was extracted with an RNeasy Micro Kit (QIAGEN). The quality of RNA in samples was checked by Agilent 2100 Bioanalyzer (Agilent Technologies).
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | cDNA was prepared from 1 μg of aRNA using the SuperScript Double Strand cDNA Synthesis Kit (Invitrogen). T7-(dT)24 primer (primer sequence: 5’GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(T)24-3’), was used for first strand cDNA synthesis (Amersham Biosciences, Tokyo, Japan). Then, biotinylated cRNA was synthesized employing the GeneChip Expression Reagents for IVT Labeling Kit (Affymetrix, USA).
| Sample_hyb_protocol | After 20 microgram of biotin-labeled cRNA was fragmented, hybridization solution was prepared using a GeneChip Eukaryotic Hybridization Control Kit (Affymetrix) and was hybridized to the Affymetrix Mouse Genome MOE430B Array for 45 ℃ for 16 hrs in GeneChip Hybridization Oven 640 (Affymetrix).
| Sample_scan_protocol | For microarray imaging data analysis, the proprietary software (GeneChip Microarray Suite, v. 5.0, Affymetrix) was used. In brief, total array normalization was performed comparing the data between hr/hr and +/hr, and normalized data were expressed as a fold change (log 10 ratio).
| Sample_data_processing | Normalization was performed in accordance with mouse maintenance genes on the Mouse Set 430A (Affymetrix).
| Sample_platform_id | GPL339
| Sample_contact_name | Tomomi,,Soshin
| Sample_contact_email | soushintmm@chugai-pharm.co.jp
| Sample_contact_phone | +81-3-5841-5401
| Sample_contact_fax | +81-3-5841-8185
| Sample_contact_laboratory | Veterinary Pathology
| Sample_contact_department | Graduate School of Agricultural and Life Sciences
| Sample_contact_institute | the University of Tokyo
| Sample_contact_address | Yayoi 1-1-1
| Sample_contact_city | Tokyo
| Sample_contact_zip/postal_code | 113-8657
| Sample_contact_country | Japan
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM154nnn/GSM154162/suppl/GSM154162.CEL.gz
| Sample_series_id | GSE6866
| Sample_data_row_count | 22690
| |
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