Search results for the GEO ID: GSE6869 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM158373 | GPL570 |
|
PCB77 treated HepG2 cells at T0, biological replicate 1
|
HepG2 cell line from ATCC at 8th generation before PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines before PCB77 treatment.
|
Sample_geo_accession | GSM158373
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 0 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158373/suppl/GSM158373.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158374 | GPL570 |
|
PCB77 treated HepG2 cells at T0, biological replicate 2
|
HepG2 cell line from ATCC at 8th generation before PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines before PCB77 treatment.
|
Sample_geo_accession | GSM158374
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 0 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158374/suppl/GSM158374.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158375 | GPL570 |
|
PCB77 treated HepG2 cells at T0, biological replicate 3
|
HepG2 cell line from ATCC at 8th generation before PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines before PCB77 treatment.
|
Sample_geo_accession | GSM158375
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 0 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158375/suppl/GSM158375.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158376 | GPL570 |
|
PCB77 treated HepG2 cells at T0.5, biological replicate 1
|
HepG2 cell line from ATCC at 8th generation after 30 minutes of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 30 minutes of PCB77 treatment.
|
Sample_geo_accession | GSM158376
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 30 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158376/suppl/GSM158376.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158377 | GPL570 |
|
PCB77 treated HepG2 cells at T0.5, biological replicate 2
|
HepG2 cell line from ATCC at 8th generation after 30 minutes of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 30 minutes of PCB77 treatment.
|
Sample_geo_accession | GSM158377
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 30 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158377/suppl/GSM158377.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158378 | GPL570 |
|
PCB77 treated HepG2 cells at T0.5, biological replicate 3
|
HepG2 cell line from ATCC at 8th generation after 30 minutes of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 30 minutes of PCB77 treatment.
|
Sample_geo_accession | GSM158378
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 30 minutes
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158378/suppl/GSM158378.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158379 | GPL570 |
|
PCB77 treated HepG2 cells at T6, biological replicate 1
|
HepG2 cell line from ATCC at 8th generation after 6 hours of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 6 hours of PCB77 treatment.
|
Sample_geo_accession | GSM158379
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 6 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158379/suppl/GSM158379.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158380 | GPL570 |
|
PCB77 treated HepG2 cells at T6, biological replicate 2
|
HepG2 cell line from ATCC at 8th generation after 6 hours of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 6 hours of PCB77 treatment.
|
Sample_geo_accession | GSM158380
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 6 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158380/suppl/GSM158380.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158381 | GPL570 |
|
PCB77 treated HepG2 cells at T6, biological replicate 3
|
HepG2 cell line from ATCC at 8th generation after 6 hours of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 6 hours of PCB77 treatment.
|
Sample_geo_accession | GSM158381
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 6 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158381/suppl/GSM158381.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158382 | GPL570 |
|
PCB77 treated HepG2 cells at T24, biological replicate 1
|
HepG2 cell line from ATCC at 8th generation after 24 hours of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 24 hours of PCB77 treatment.
|
Sample_geo_accession | GSM158382
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 24 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158382/suppl/GSM158382.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
| |
|
GSM158383 | GPL570 |
|
PCB77 treated HepG2 cells at T24, biological replicate 2
|
HepG2 cell line from ATCC at 8th generation after 24 hours of PCB77 treatment.
|
genotype: Male
age: 15 years adolescent male
|
Gene expression data from HepG2 cell lines after 24 hours of PCB77 treatment.
|
Sample_geo_accession | GSM158383
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 24 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158383/suppl/GSM158383.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
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GSM158384 | GPL570 |
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PCB77 treated HepG2 cells at T24, biological replicate 3
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HepG2 cell line from ATCC at 8th generation after 24 hours of PCB77 treatment.
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genotype: Male
age: 15 years adolescent male
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Gene expression data from HepG2 cell lines after 24 hours of PCB77 treatment.
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Sample_geo_accession | GSM158384
| Sample_status | Public on Jul 22 2010
| Sample_submission_date | Jan 25 2007
| Sample_last_update_date | Jul 22 2010
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_treatment_protocol_ch1 | Treated with PCB77 for 24 hours
| Sample_growth_protocol_ch1 | Grown for 48 hours in DMEM supplemented with 10% FBS and 1X Penicillin-Streptromycin
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
| Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
| Sample_platform_id | GPL570
| Sample_contact_name | Sisir,K.,Dutta
| Sample_contact_email | sdutta@howard.edu
| Sample_contact_phone | 202-806-6942
| Sample_contact_fax | 202-806-5832
| Sample_contact_laboratory | Molecular Genetics
| Sample_contact_department | Biology
| Sample_contact_institute | Howard University
| Sample_contact_address | 415 College Street, NW
| Sample_contact_city | Washington DC
| Sample_contact_state | DC
| Sample_contact_zip/postal_code | 20059
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM158nnn/GSM158384/suppl/GSM158384.CEL.gz
| Sample_series_id | GSE6869
| Sample_series_id | GSE6878
| Sample_data_row_count | 54675
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