Search results for the GEO ID: GSE6960  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM160468 | GPL570 |
|
Control A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160468
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160468/suppl/GSM160468.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160469 | GPL570 |
|
Control B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160469
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160469/suppl/GSM160469.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160470 | GPL570 |
|
Control C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160470
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160470/suppl/GSM160470.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160471 | GPL570 |
|
Zinc A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160471
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160471/suppl/GSM160471.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160472 | GPL570 |
|
Zinc B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160472
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160472/suppl/GSM160472.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160473 | GPL570 |
|
Zinc C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160473
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160473/suppl/GSM160473.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160474 | GPL570 |
|
PCI 5002 A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160474
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160474/suppl/GSM160474.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160475 | GPL570 |
|
PCI 5002 B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160475
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160475/suppl/GSM160475.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160476 | GPL570 |
|
PCI 5002 C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160476
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160476/suppl/GSM160476.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160477 | GPL570 |
|
PCI 5002 Zinc A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160477
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160477/suppl/GSM160477.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160478 | GPL570 |
|
PCI 5002 Zinc B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160478
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160478/suppl/GSM160478.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160479 | GPL570 |
|
PCI 5002 Zinc C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160479
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160479/suppl/GSM160479.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
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