Search results for the GEO ID: GSE6972  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM160468 | GPL570 |
|
Control A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160468
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160468/suppl/GSM160468.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160469 | GPL570 |
|
Control B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160469
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160469/suppl/GSM160469.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160470 | GPL570 |
|
Control C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160470
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, control (5% mannitol) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160470/suppl/GSM160470.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160471 | GPL570 |
|
Zinc A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160471
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160471/suppl/GSM160471.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160472 | GPL570 |
|
Zinc B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160472
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160472/suppl/GSM160472.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160473 | GPL570 |
|
Zinc C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160473
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160473/suppl/GSM160473.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160474 | GPL570 |
|
PCI 5002 A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160474
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160474/suppl/GSM160474.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160475 | GPL570 |
|
PCI 5002 B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160475
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160475/suppl/GSM160475.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160476 | GPL570 |
|
PCI 5002 C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160476
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160476/suppl/GSM160476.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160477 | GPL570 |
|
PCI 5002 Zinc A
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160477
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160477/suppl/GSM160477.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160478 | GPL570 |
|
PCI 5002 Zinc B
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160478
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160478/suppl/GSM160478.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160479 | GPL570 |
|
PCI 5002 Zinc C
|
non-cycling plateau phase A549 lung cancer cell culture
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
|
| Sample_geo_accession | GSM160479
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_growth_protocol_ch1 | A549 human lung cancer cells (1 x 10^5 cells per T-25 flask in 7 mL complete RPMI 1640 medium) were seeded 8 days prior to treatment of non-cycling plateau phase cultures with drug. At 4 hours prior to RNA isolation, PCI-5002 (10 μM final concentration) + ZnOAc2 (25 μM final concentration) solution was added to the cultures. After incubation, cultures were washed twice with HBSS supplemented with 0.5% BSA and total RNA was isolated.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160479/suppl/GSM160479.CEL.gz
| | Sample_series_id | GSE6960
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160486 | GPL570 |
|
A549 Xenograft Control A
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160486
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160486/suppl/GSM160486.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160488 | GPL570 |
|
A549 Xenograft Control B
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160488
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160488/suppl/GSM160488.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160490 | GPL570 |
|
A549 Xenograft Control C
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160490
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160490/suppl/GSM160490.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160493 | GPL570 |
|
A549 Xenograft Control D
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160493
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with 5% mannitol (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160493/suppl/GSM160493.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160499 | GPL570 |
|
A549 Xenograft PCI 5002 A
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160499
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160499/suppl/GSM160499.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160501 | GPL570 |
|
A549 Xenograft PCI 5002 B
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160501
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160501/suppl/GSM160501.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160504 | GPL570 |
|
A549 Xenograft PCI 5002 C
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160504
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160504/suppl/GSM160504.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160507 | GPL570 |
|
A549 Xenograft PCI 5002 D
|
A
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160507
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5002 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160507/suppl/GSM160507.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160510 | GPL570 |
|
A549 Xenograft PCI 5003 A
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160510
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160510/suppl/GSM160510.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160512 | GPL570 |
|
A549 Xenograft PCI 5003 B
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160512
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160512/suppl/GSM160512.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160515 | GPL570 |
|
A549 Xenograft PCI 5003 C
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160515
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160515/suppl/GSM160515.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
|
GSM160517 | GPL570 |
|
A549 Xenograft PCI 5003 D
|
A549 human lung cancer cell xenograft in CD-1 nude mice
|
a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) = a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
|
| Sample_geo_accession | GSM160517
| | Sample_status | Public on Feb 07 2007
| | Sample_submission_date | Feb 06 2007
| | Sample_last_update_date | Feb 06 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_biomaterial_provider_ch1 | ATCC
| | Sample_treatment_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_growth_protocol_ch1 = 1.25 million A549 cells were injected subcutaneously/intramuscularly into the right hind flank of 6 week old CD-1 nude mice that had been irradiated with 4 Gy of total body irradiation from a 137Cs radiation source one day prior to tumor implantation. When the average size of tumors reached approximately 100 mm3, mice were randomized by tumor size to treatment groups, typically containing 6-8 mice per group. Tumor and body weight measurements were performed three times per week. Tumor volume was calculated using the equation V (mm3) | a x b2/2, where a is the largest diameter and b is the smallest diameter. No significant body weight loss was observed. To perform gene expression profiling, mice were treated intravenously with one dose (100 μmol/kg) PCI-5003 (4 mice per group) when the average A549 tumor size reached 500 mm3. After four hours, tumors were harvested and snap frozen immediately on dry ice. Tumor tissue was homogenized, and total RNA was isolated and subjected to analysis using Human Genome U133 Plus 2.0 Arrays.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | We used the RNA Stat-60 reagent (Tel-Test, Inc.) according to the manufacturer's recomended protocols.
| | Sample_label_ch1 | Affymetrix single-stage biotin
| | Sample_label_protocol_ch1 | We used GeneChip® One-Cycle Target Labeling and Control Reagents according to the manufacturer's recomended protocols.
| | Sample_hyb_protocol | We used the Affymetrix Hybridization Oven 640 and GeneChip® Fluidics Station 450 according to the manufacturer's recomended protocols.
| | Sample_scan_protocol | We used the Affymetrix GeneChip® Scanner 3000 according to the manufacturer's recomended protocols.
| | Sample_data_processing | RMA Normalization
| | Sample_platform_id | GPL570
| | Sample_contact_name | Joseph,Gerard,Hacia
| | Sample_contact_email | hacia@hsc.usc.edu
| | Sample_contact_phone | 323-442-3030
| | Sample_contact_fax | 323-442-2764
| | Sample_contact_laboratory | Hacia Lab
| | Sample_contact_department | Biochemistry and Molecular Biology
| | Sample_contact_institute | University of Southern California
| | Sample_contact_address | 2250 Alcazar Street, IGM 240
| | Sample_contact_city | Los Angeles
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 90089
| | Sample_contact_country | USA
| | Sample_contact_web_link | www.usc.edu/pibbs/faculty/hacia_j.htm
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM160nnn/GSM160517/suppl/GSM160517.CEL.gz
| | Sample_series_id | GSE6962
| | Sample_series_id | GSE6972
| | Sample_data_row_count | 54675
| | |
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