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Search results for the GEO ID: GSE7002
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GSM159724
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 5d ID138 EB_07_5d_138_Formaldehyde_RatNasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.732 OD280: 0.907 Ratio: 1.89 Dilution: 1:5 RNA Integrity Number: 7.9 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 138 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159725
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 5d ID139 EB_07_5d_139_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.469 OD280: 0.766 Ratio: 1.89 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 139 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159726
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 5d ID140 EB_07_5d_140_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.434 OD280: 1.353 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 7.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 140 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159727
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 6h ID127 EB_07_6h_127_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.588 OD280: 0.789 Ratio: 1.99 Dilution: 1:5 RNA Integrity Number: 8.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 127 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hour time point
GSM159728
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 6h ID129 EB_07_6h_129_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.946 OD280: 0.992 Ratio: 1.92 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 129 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hour time point
GSM159729
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 6h ID287 EB_6_6h_287_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.435 OD280: 0.745 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 287 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hour time point
GSM159730
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 8d ID298 EB_6_8d_298_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.708 OD280: 0.892 Ratio: 1.89 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 298 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159731
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 8d ID299 EB_6_8d_299_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.536 OD280: 0.766 Ratio: 1.98 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 299 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159732
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 8d ID300 EB_6_8d_300_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 0.977 OD280: 0.478 Ratio: 1.99 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 300 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159733
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 8d ID301 EB_6_8d_301_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 0.918 OD280: 0.454 Ratio: 1.99 Dilution: 1:5 RNA Integrity Number: 7.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 301 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159734
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 5d ID293 EB_6_5d_293_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.674 OD280: 0.969 Ratio: 1.87 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 293 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159735
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 5d ID294 EB_6_5d_294_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.683 OD280: 0.839 Ratio: 1.98 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 294 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159736
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 5d ID296 EB_6_5d_296_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.254 OD280: 0.641 Ratio: 1.98 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 296 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159737
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 5d ID297 EB_6_5d_297_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.229 OD280: 1.143 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 297 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159738
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 6h ID283 EB_6_6h_283_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.371 OD280: 0.727 Ratio: 1.87 Dilution: 1:5 RNA Integrity Number: 7.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 283 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hour time point
GSM159739
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 6h ID284 EB_6_6h_284_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.092 OD280: 0.567 Ratio: 1.89 Dilution: 1:5 RNA Integrity Number: 7.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 284 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hour time point
GSM159740
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 6h ID286 EB_6_6h_286_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.04 OD280: 0.53 Ratio: 1.97 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 286 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hour time point
GSM159741
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 19d ID309 EB_6_19d_309_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.448 OD280: 0.781 Ratio: 1.85 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 309 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159742
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 19d ID310 EB_6_19d_310_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 0.881 OD280: 0.457 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 310 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159743
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 19d ID312 EB_6_19d_312_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.315 OD280: 0.668 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 8.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 312 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159744
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 24h ID288 EB_6_24h_288_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.242 OD280: 1.1 Ratio: 1.97 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 288 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hrs per day, 24 h time point
GSM159745
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 24h ID289 EB_6_24h_289_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.088 OD280: 0.579 Ratio: 1.8 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 289 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hrs per day, 24 h time point
GSM159746
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 24h ID290 EB_6_24h_290_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.853 OD280: 0.955 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 290 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hrs per day, 24 h time point
GSM159747
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 24h ID292 EB_6_24h_292_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.326 OD280: 0.691 Ratio: 1.83 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 292 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hrs per day, 24 h time point
GSM159748
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 8d ID221 EB_2_8d_221_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.539 OD280: 1.303 Ratio: 1.91 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 221 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159749
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 8d ID222 EB_2_8d_222_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.381 OD280: 0.716 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 222 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159750
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 8d ID223 EB_2_8d_223_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.226 OD280: 1.154 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 7.9 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 223 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159751
GPL1355
Nasal Epithelium Instilation Formaldehyde 400mM 6h ID365 EB_400_6_365_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.054 OD280: 1.504 Ratio: 1.99 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 365 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, 400 mM formaldehyde, single exposure, 6 h time point
GSM159752
GPL1355
Nasal Epithelium Instilation Formaldehyde 400mM 6h ID369 EB_400_6_369_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.572 OD280: 1.326 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 369 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, 400 mM formaldehyde, single exposure, 6 h time point
GSM159753
GPL1355
Nasal Epithelium Instilation Formaldehyde 400mM 6h ID372 EB_400_6_372_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.811 OD280: 0.9 Ratio: 1.97 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 372 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, 400 mM formaldehyde, single exposure, 6 h time point
GSM159754
GPL1355
Nasal Epithelium Instilation Formaldehyde 400mM 6h ID374 EB_400_6_374_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.236 OD280: 1.154 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 374 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, 400 mM formaldehyde, single exposure, 6 h time point
GSM159755
GPL1355
Nasal Epithelium Inhalation Formaldehyde 6ppm 19d ID308 EB_6_19d_308_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.386 OD280: 0.669 Ratio: 2.01 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 308 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 6 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159756
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 5d ID219 EB_2_5d_219_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.681 OD280: 0.848 Ratio: 1.97 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 219 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159757
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 6h ID205 EB_2_6h_205_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.442 OD280: 1.248 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 205 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 h time point
GSM159758
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 6h ID206 EB_2_6h_206_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.72 OD280: 0.901 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 206 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 h time point
GSM159759
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 6h ID207 EB_2_6h_207_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 0.848 OD280: 0.434 Ratio: 1.97 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 207 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 h time point
GSM159760
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 6h ID209 EB_2_6h_209_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 0.898 OD280: 0.49 Ratio: 1.84 Dilution: 1:5 RNA Integrity Number: 7.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 209 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 h time point
GSM159761
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 8d ID220 EB_2_8d_220_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.749 OD280: 0.86 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 220 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159762
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 24h ID211 EB_2_24h_211_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.377 OD280: 0.716 Ratio: 1.86 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 211 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hrs per day, 24 h time point
GSM159763
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 24h ID212 EB_2_24h_212_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.124 OD280: 0.574 Ratio: 1.86 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 212 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hrs per day, 24 h time point
GSM159764
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 24h ID213 EB_2_24h_213_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.607 OD280: 0.846 Ratio: 1.83 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 213 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hrs per day, 24 h time point
GSM159765
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 24h ID214 EB_2_24h_214_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.172 OD280: 1.137 Ratio: 1.87 Dilution: 1:5 RNA Integrity Number: 8.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 214 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hrs per day, 24 h time point
GSM159766
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 5d ID215 EB_2_5d_215_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.652 OD280: 0.857 Ratio: 1.92 Dilution: 1:5 RNA Integrity Number: 7.9 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 215 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159767
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 5d ID216 EB_2_5d_216_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.042 OD280: 1.079 Ratio: 1.95 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 216 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159768
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 5d ID217 EB_2_5d_217_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.15 OD280: 0.539 Ratio: 2.03 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 217 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159769
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 5d ID218 EB_2_5d_218_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.03 OD280: 1.028 Ratio: 1.96 Dilution: 1:5 RNA Integrity Number: 7.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 218 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 5 day time point
GSM159770
GPL1355
Nasal Epithelium Inhalation Formaldehyde 15ppm 6h ID338 EB_15_6_338_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.975 OD280: 1.082 Ratio: 1.86 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 338 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 15 ppm, 6 h time point
GSM159771
GPL1355
Nasal Epithelium Inhalation Formaldehyde 15ppm 6h ID341 EB_15_6_341_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.592 OD280: 1.273 Ratio: 2.03 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 341 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 15 ppm, 6 h time point
GSM159772
GPL1355
Nasal Epithelium Inhalation Formaldehyde 15ppm 6h ID342 EB_15_6_342_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.408 OD280: 1.228 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 342 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 15 ppm, 6 h time point
GSM159773
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 19d ID230 EB_2_19d_230_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.232 OD280: 0.618 Ratio: 1.95 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 230 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159774
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 19d ID232 EB_2_19d_232_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.515 OD280: 0.791 Ratio: 1.89 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 232 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159775
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 19d ID233 EB_2_19d_233_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.929 OD280: 1.018 Ratio: 1.86 Dilution: 1:5 RNA Integrity Number: 8.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 233 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159776
GPL1355
Nasal Epithelium Inhalation Formaldehyde 2ppm 19d ID234 EB_2_19d_234_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.675 OD280: 0.838 Ratio: 1.96 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 234 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159777
GPL1355
Nasal Epithelium Instilation Formaldehyde 0mM 6h ID355 EB_0_6_355_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.469 OD280: 1.802 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 355 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, vehicle control, single exposure, 6 h time point
GSM159778
GPL1355
Nasal Epithelium Instilation Formaldehyde 0mM 6h ID356 EB_0_6_356_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.908 OD280: 1.959 Ratio: 1.96 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 356 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, vehicle control, single exposure, 6 h time point
GSM159779
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 8d ID64 EB_0_8d_64_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.222 OD280: 1.139 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 64 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 8 day time point
GSM159780
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 8d ID65 EB_0_8d_65_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.743 OD280: 0.932 Ratio: 1.84 Dilution: 1:5 RNA Integrity Number: 8.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 65 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 8 day time point
GSM159781
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 8d ID66 EB_0_8d_66_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.318 OD280: 1.187 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 66 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 8 day time point
GSM159782
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 8d ID67 EB_0_8d_67_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.007 OD280: 0.497 Ratio: 1.97 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 67 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 8 day time point
GSM159783
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 8d ID68 EB_0_8d_68_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.644 OD280: 1.383 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 68 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 8 day time point
GSM159784
GPL1355
Nasal Epithelium Inhalation Formaldehyde 15ppm 6h ID337 EB_15_6_337_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.417 OD280: 1.217 Ratio: 1.98 Dilution: 1:5 RNA Integrity Number: 7.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 337 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 15 ppm, 6 h time point
GSM159785
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID53 EB_0_6h_53_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.708 OD280: 0.905 Ratio: 1.86 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 53 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159786
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID321 EB_0_6_321_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.245 OD280: 1.679 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 7.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 321 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159787
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID322 EB_0_6_322_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.023 OD280: 1.564 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 7.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 322 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159788
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID323 EB_0_6_323_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.413 OD280: 1.719 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 7.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 323 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159789
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID325 EB_0_6_325_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 4.08 OD280: 2.003 Ratio: 2.01 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 325 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159790
GPL1355
Nasal Epithelium Instilation Formaldehyde 0mM 6h ID352 EB_0_6_352_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.678 OD280: 1.857 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 7.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 352 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, vehicle control, single exposure, 6 h time point
GSM159791
GPL1355
Nasal Epithelium Instilation Formaldehyde 0mM 6h ID354 EB_0_6_354_HCHO_nasal_Rat230_2_EB_HCHO_Rat_Nasal Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 3.701 OD280: 1.82 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 354 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde instilation exposure, vehicle control, single exposure, 6 h time point
GSM159792
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 5d ID60 EB_0_5d_60_Formaldehyde_RatNasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.163 OD280: 0.612 Ratio: 1.87 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 60 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 5 day time point
GSM159793
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 5d ID61 EB_0_5d_61_Formaldehyde_RatNasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.894 OD280: 0.979 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 61 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 5 day time point
GSM159794
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 5d ID62 EB_0_5d_62_Formaldehyde_RatNasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.542 OD280: 0.787 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 7.7 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 62 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 5 day time point
GSM159795
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 5d ID63 EB_0_5d_63_Formaldehyde_RatNasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.549 OD280: 0.803 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 7.9 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 63 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 5 day time point
GSM159796
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID49 EB_0_6h_49_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.692 OD280: 1.387 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 49 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159797
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID50 EB_0_6h_50_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.242 OD280: 1.132 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 50 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159798
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 6h ID52 EB_0_6h_52_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.843 OD280: 0.95 Ratio: 1.9 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 52 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 h time point
GSM159799
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 19d ID75 EB_0_19d_75_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.626 OD280: 0.845 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 75 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 19 day time point
GSM159800
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 19d ID76 EB_0_19d_76_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.748 OD280: 0.861 Ratio: 2.01 Dilution: 1:5 RNA Integrity Number: 8.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 76 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 19 day time point
GSM159801
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 19d ID77 EB_0_19d_77_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.808 OD280: 0.956 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 77 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 19 day time point
GSM159802
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 24h ID54 EB_0_24h_54_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.782 OD280: 1.422 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 54 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 24 h time point
GSM159803
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 24h ID56 EB_0_24h_56_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.899 OD280: 1.476 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 7.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 56 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 24 h time point
GSM159804
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 24h ID57 EB_0_24h_57_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.468 OD280: 1.278 Ratio: 1.88 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 57 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 24 h time point
GSM159805
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 24h ID58 EB_0_24h_58_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.509 OD280: 0.793 Ratio: 1.84 Dilution: 1:5 RNA Integrity Number: 7.9 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 58 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 24 h time point
GSM159806
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 6h ID130 EB_07_6h_130_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.621 OD280: 0.822 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 130 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hour time point
GSM159807
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 6h ID131 EB_07_6h_131_Formaldehyde_RatNasalTissue_CIIT05024_Rat230_2 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.04 OD280: 1.071 Ratio: 1.88 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 131 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 6, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hour time point
GSM159808
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 8d ID143 EB_07_8d_143_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.793 OD280: 0.877 Dilution: 1:5 RNA Integrity Number: 8.5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 143 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159809
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 8d ID144 EB_07_8d_144_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.265 OD280: 1.203 Ratio: 1.85 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 144 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159810
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 8d ID145 EB_07_8d_145_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.606 OD280: 0.744 Ratio: 2.12 Dilution: 1:5 RNA Integrity Number: 8.2 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 145 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159811
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 8d ID146 EB_07_8d_146_HCHO_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.631 OD280: 0.833 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 146 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 8, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 8 day time point
GSM159812
GPL1355
Nasal Epithelium Inhalation Formaldehyde 0ppm 19d ID73 EB_0_19d_73_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.615 OD280: 0.851 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 73 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, air control, 6 hours per day, 5 days per week, 19 day time point
GSM159813
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 19d ID152 EB_07_19d_152_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.221 OD280: 0.621 Ratio: 1.96 Dilution: 1:5 RNA Integrity Number: 8.4 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 152 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159814
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 19d ID154 EB_07_19d_154_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.139 OD280: 0.58 Ratio: 1.95 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 154 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159815
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 19d ID155 EB_07_19d_155_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.475 OD280: 0.755 Ratio: 1.93 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 155 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159816
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 19d ID156 EB_07_19d_156_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.809 OD280: 0.918 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 8.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 156 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 19 day time point
GSM159817
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 24h ID132 EB_07_24h_132_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.789 OD280: 0.949 Ratio: 1.83 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 132 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 24 h time point
GSM159818
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 24h ID133 EB_07_24h_133_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.767 OD280: 1.439 Ratio: 1.87 Dilution: 1:5 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 133 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 24 h time point
GSM159819
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 24h ID134 EB_07_24h_134_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 2.167 OD280: 1.069 Ratio: 1.95 Dilution: 1:5 RNA Integrity Number: 8.3 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 134 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 24 h time point
GSM159820
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 24h ID136 EB_07_24h_136_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.301 OD280: 0.666 Ratio: 1.88 Dilution: 1:5 RNA Integrity Number: 7.8 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 136 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 24, Units of Harvest Time Point: hrs nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 24 h time point
GSM159821
GPL1355
Nasal Epithelium Inhalation Formaldehyde 07ppm 5d ID137 EB_07_5d_137_Formaldehyde_Nasal_Rat230_2_CIIT05024 Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.814 OD280: 0.932 Ratio: 1.91 Dilution: 1:5 RNA Integrity Number: 8.1 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 137 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 5, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 0.7 ppm, 6 hours per day, 5 days per week, 5 day time point
 
 
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