Search results for the GEO ID: GSE7009 ![](/q11.jpeg) |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM161670 | GPL96 |
|
HeLa cells control 1 (Luciferase siRNA transfection)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the first control (luciferase siRNA transfection).
|
Sample_geo_accession | GSM161670
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161670/suppl/GSM161670.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161670/suppl/GSM161670.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
|
GSM161671 | GPL96 |
|
HeLa cells control 2 (Luciferase siRNA transfection)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the second control (luciferase siRNA transfection).
|
Sample_geo_accession | GSM161671
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161671/suppl/GSM161671.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161671/suppl/GSM161671.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
|
GSM161674 | GPL96 |
|
HeLa cells control 3 (Luciferase siRNA transfection)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the third control (luciferase siRNA transfection).
|
Sample_geo_accession | GSM161674
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161674/suppl/GSM161674.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161674/suppl/GSM161674.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
|
GSM161678 | GPL96 |
|
HeLa cells transfected with UPF1 siRNA 1 (experiment)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the first experiment (UPF1 siRNA transfection).
|
Sample_geo_accession | GSM161678
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161678/suppl/GSM161678.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161678/suppl/GSM161678.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
|
GSM161679 | GPL96 |
|
HeLa cells transfected with UPF1 siRNA 2 (experiment)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the second experiment (UPF1 siRNA transfection).
|
Sample_geo_accession | GSM161679
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161679/suppl/GSM161679.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161679/suppl/GSM161679.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
|
GSM161686 | GPL96 |
|
HeLa cells transfected with UPF1 siRNA 3 (experiment)
|
HeLa cells, 72 hs after transfection
|
HeLa cells
(cervical cancer cells)
|
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the third experiment (UPF1 siRNA transfection).
|
Sample_geo_accession | GSM161686
| Sample_status | Public on Mar 01 2007
| Sample_submission_date | Feb 12 2007
| Sample_last_update_date | Feb 13 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Homo sapiens
| Sample_taxid_ch1 | 9606
| Sample_molecule_ch1 | cytoplasmic RNA
| Sample_extract_protocol_ch1 | protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Standard Affymetrix protocol
| Sample_hyb_protocol | Standard Affymetrix protocol
| Sample_scan_protocol | Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
| Sample_data_processing | We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
| Sample_platform_id | GPL96
| Sample_contact_name | Marcelo,,Viegas
| Sample_contact_email | viegas@embl.de
| Sample_contact_laboratory | Kulozik/Hentze
| Sample_contact_institute | EMBL-MMPU
| Sample_contact_address | IM NEUENHEIMER FELD 156
| Sample_contact_city | Heidelberg
| Sample_contact_zip/postal_code | 69120
| Sample_contact_country | Germany
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161686/suppl/GSM161686.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM161nnn/GSM161686/suppl/GSM161686.CHP.gz
| Sample_series_id | GSE7009
| Sample_data_row_count | 22283
| |
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