Search results for the GEO ID: GSE7038
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GSM ID
GPL ID
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Title
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Description
Characteristics
GSM162807
GPL1261
MMH-D3 cell line rep1 MMH-D3 line Hepatocytic differentiated cell line MMH-D3 characterised by L. Amicone et al. (Amicone L, Spagnoli FM, Spath G, Giordano S, Tommasini C, Bernardini S, De Luca V, et al. Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes. Embo J 1997;16:495-503). Synthesis of biotinylated cRNA targets, arrays hybridization (Mouse430_2 GeneChip, Affymetrix, Santa Clara,CA), staining and scanning were performed using Affymetrix standard protocols starting from 5μg of total RNA.
GSM162863
GPL1261
WTE14/1 RLSC cell line resident liver stem cells (RLSCs) To isolate RLSCs we used the same culture conditions previously described for the establishment of MMH lines (Amicone L, Spagnoli FM, Spath G, Giordano S, Tommasini C, Bernardini S, De Luca V, et al. Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes. Embo J 1997;16:495-503), selecting for “palmate” morphology. Primary cultures were prepared from livers of cyto-Met transgenic embryo 14 dpc (MetE14). Synthesis of biotinylated cRNA targets, arrays hybridization (Mouse430_2 GeneChip, Affymetrix, Santa Clara,CA), staining and scanning were performed using Affymetrix standard protocols starting from 5μg of total RNA.
GSM216535
GPL1261
MetE14/3 RLSC cell line (B2 clone) WT E14/3 resident liver stem cells (RLSCs) To isolate RLSCs we used the same culture conditions previously described for the establishment of MMH lines (Amicone L, Spagnoli FM, Spath G, Giordano S, Tommasini C, Bernardini S, De Luca V, et al. Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes. Embo J 1997;16:495-503), selecting for “palmate” morphology. Primary cultures were prepared from livers of embryo 14 dpc (WT E14). Synthesis of biotinylated cRNA targets, arrays hybridization (Mouse430_2 GeneChip, Affymetrix, Santa Clara,CA), staining and scanning were performed using Affymetrix standard protocols starting from 5μg of total RNA.
GSM216536
GPL1261
MMH-D3 cell line rep2 MMH-D3 line Hepatocytic differentiated cell line MMH-D3 characterised by L. Amicone et al. (Amicone L, Spagnoli FM, Spath G, Giordano S, Tommasini C, Bernardini S, De Luca V, et al. Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes. Embo J 1997;16:495-503). Synthesis of biotinylated cRNA targets, arrays hybridization (Mouse430_2 GeneChip, Affymetrix, Santa Clara,CA), staining and scanning were performed using Affymetrix standard protocols starting from 5μg of total RNA.
 
 
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