Search results for the GEO ID: GSE7041  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM162914 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- Control_A72
|
Rat Lung
|
Genotype: Control Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162914
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162914/suppl/GSM162914.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162915 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- Control_A73
|
Rat Lung
|
Genotype: Control Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162915
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162915/suppl/GSM162915.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162916 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- Control_A81
|
Rat Lung
|
Genotype: Control Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162916
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162916/suppl/GSM162916.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162917 | GPL1355 |
|
Brown Norway Rat Lung- Control_B30
|
Rat Lung
|
Genotype: Control Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162917
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162917/suppl/GSM162917.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162918 | GPL1355 |
|
Brown Norway Rat Lung- Control_B31
|
Rat Lung
|
Genotype: Control Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162918
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162918/suppl/GSM162918.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162919 | GPL1355 |
|
Brown Norway Rat Lung- Control_B32
|
Rat Lung
|
Genotype: Control Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162919
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162919/suppl/GSM162919.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162920 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- HTV Ventilation_A69
|
Rat Lung
|
Genotype: HTV Ventilated Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162920
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162920/suppl/GSM162920.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162921 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- HTV Ventilation_A70
|
Rat Lung
|
Genotype: HTV Ventilated Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162921
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162921/suppl/GSM162921.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162922 | GPL1355 |
|
Dahl Salt Sensitive Rat Lung- HTV Ventilation_A82
|
Rat Lung
|
Genotype: HTV Ventilated Dahl Salt Sensitive Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162922
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162922/suppl/GSM162922.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162923 | GPL1355 |
|
Brown Norway Rat Lung- HTV Ventilated_B36
|
Rat Lung
|
Genotype: HTV Ventilated Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162923
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162923/suppl/GSM162923.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162924 | GPL1355 |
|
Brown Norway Rat Lung- HTV Ventilated_B37
|
Rat Lung
|
Genotype: HTV Ventilated Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162924
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162924/suppl/GSM162924.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
|
GSM162925 | GPL1355 |
|
Brown Norway Rat Lung- HTV Ventilated_B40
|
Rat Lung
|
Genotype: HTV Ventilated Brown Norway Rats
Weights: 250-350 grams
|
Gene expression data from rat lungs exposed to room air (ventilation)
|
| Sample_geo_accession | GSM162925
| | Sample_status | Public on Feb 15 2007
| | Sample_submission_date | Feb 15 2007
| | Sample_last_update_date | Feb 15 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_treatment_protocol_ch1 | At the termination of each experiment, all animals were euthanized by exsanguination under anesthesia in accordance with institutional guidelines. The pulmonary vasculature was perfused via the pulmonary artery with sterile phosphate-buffered saline (PBS) and the right lung was clamped at the level of the mainstem bronchus, excised, and snap frozen in liquid nitrogen for subsequent RNA analysis/expression profiling. Right lung mRNA was isolated from 3 samples per strain and condition (as an example, we analyzed 3 SS rat strains exposed to control, and 3 SS rat strains exposed to HTV for expression profiling).
| | Sample_growth_protocol_ch1 | Not Applicable
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
| | Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Ankit,A,Desai
| | Sample_contact_email | adesai1@gmail.com
| | Sample_contact_phone | 312-523-7931
| | Sample_contact_fax | 773-834-2687
| | Sample_contact_laboratory | Joe G.N. Garcia Lab / Center for Integrative Science
| | Sample_contact_department | Medicine
| | Sample_contact_institute | The University of Chicago
| | Sample_contact_address | 929 East 57th Street, Room W403R
| | Sample_contact_city | Chicago
| | Sample_contact_state | IL
| | Sample_contact_zip/postal_code | 60637
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM162nnn/GSM162925/suppl/GSM162925.CEL.gz
| | Sample_series_id | GSE7041
| | Sample_data_row_count | 31099
| | |
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