Search results for the GEO ID: GSE7095  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM170692 | GPL201 |
|
LPS-activated DCs, Donor A (A1)
|
Monocyte-derived dendritic cells
|
LPS-activated DCs, Donor A
|
Monocyte-derived DCs from donor A were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS treatment.
|
| Sample_geo_accession | GSM170692
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were treated with LPS (10 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170692/suppl/GSM170692.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170693 | GPL201 |
|
Viral IL-10 treated LPS-activated DCs, Donor A (A2)
|
Monocyte-derived dendritic cells
|
Viral IL-10 treated LPS-activated DCs, Donor A
|
Monocyte-derived DCs from donor A were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170693
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and cmv IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170693/suppl/GSM170693.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170694 | GPL201 |
|
Untreated immature DCs, Donor A (A4)
|
Monocyte-derived dendritic cells
|
Untreated immature DCs, Donor A
|
Monocyte-derived DCs from donor A were left unstimulated. An aliquot of cells were assayed by FACS to ensure the immature phenotype of the dendritic cells.
|
| Sample_geo_accession | GSM170694
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | none
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170694/suppl/GSM170694.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170695 | GPL201 |
|
LPS-activated DCs, Donor B (B1)
|
Monocyte-derived dendritic cells
|
LPS-activated DCs, Donor B
|
Monocyte-derived DCs from donor B were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS treatment.
|
| Sample_geo_accession | GSM170695
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were treated with LPS (10 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170695/suppl/GSM170695.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170696 | GPL201 |
|
Viral IL-10 treated LPS-activated DCs, Donor B (B2)
|
Monocyte-derived dendritic cells
|
Viral IL-10 treated LPS-activated DCs, Donor B
|
Monocyte-derived DCs from donor B were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170696
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and cmv IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170696/suppl/GSM170696.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170697 | GPL201 |
|
Untreated immature DCs, Donor B (B4)
|
Monocyte-derived dendritic cells
|
Untreated immature DCs, Donor B
|
Monocyte-derived DCs from donor B were left unstimulated. An aliquot of cells were assayed by FACS to ensure the immature phenotype of the dendritic cells.
|
| Sample_geo_accession | GSM170697
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | none
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170697/suppl/GSM170697.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170698 | GPL201 |
|
LPS-activated DCs, Donor D (D1)
|
Monocyte-derived dendritic cells
|
LPS-activated DCs, Donor D
|
Monocyte-derived DCs from donor D were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS treatment.
|
| Sample_geo_accession | GSM170698
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were treated with LPS (10 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170698/suppl/GSM170698.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170699 | GPL201 |
|
Viral IL-10 treated LPS-activated DCs, Donor D (D2)
|
Monocyte-derived dendritic cells
|
Viral IL-10 treated LPS-activated DCs, Donor D
|
Monocyte-derived DCs from donor D were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170699
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and cmv IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170699/suppl/GSM170699.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170700 | GPL201 |
|
Human IL-10 treated LPS-activated DCs, Donor D (D3)
|
Monocyte-derived dendritic cells
|
Human IL-10 treated LPS-activated DCs, Donor D
|
Monocyte-derived DCs from donor D were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170700
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and human IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170700/suppl/GSM170700.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170701 | GPL201 |
|
Untreated immature DCs, Donor D (D4)
|
Monocyte-derived dendritic cells
|
Untreated immature DCs, Donor D
|
Monocyte-derived DCs from donor D were left unstimulated. An aliquot of cells were assayed by FACS to ensure the immature phenotype of the dendritic cells.
|
| Sample_geo_accession | GSM170701
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | none
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170701/suppl/GSM170701.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170702 | GPL201 |
|
LPS-activated DCs, Donor E (E1)
|
Monocyte-derived dendritic cells
|
LPS-activated DCs, Donor E
|
Monocyte-derived DCs from donor E were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS treatment.
|
| Sample_geo_accession | GSM170702
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were treated with LPS (10 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170702/suppl/GSM170702.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170703 | GPL201 |
|
Viral IL-10 treated LPS-activated DCs, Donor E (E2)
|
Monocyte-derived dendritic cells
|
Viral IL-10 treated LPS-activated DCs, Donor E
|
Monocyte-derived DCs from donor E were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170703
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and cmv IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170703/suppl/GSM170703.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
|
GSM170704 | GPL201 |
|
Human IL-10 treated LPS-activated DCs, Donor E (E3)
|
Monocyte-derived dendritic cells
|
Human IL-10 treated LPS-activated DCs, Donor E
|
Monocyte-derived DCs from donor E were activated with LPS for 12 h. An aliquot of cells were assayed by FACS to ensure the mature phenotype of the dendritic cells after LPS and IL-10 treatment.
|
| Sample_geo_accession | GSM170704
| | Sample_status | Public on Feb 22 2007
| | Sample_submission_date | Feb 21 2007
| | Sample_last_update_date | Feb 21 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | cells were concomitantly treated with LPS (10 ng/ml) and human IL-10 (50 ng/ml) for 12 hours.
| | Sample_growth_protocol_ch1 | Isolated CD14+ monocytes were cultured with GM-CSF and IL-4 for 6 days to generate dendritic cells.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from cultured dendritic cells using the Qiagen RNeasy Mini Kit according the manufacturer’s instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared from 10 micrograms of total RNA according to the standard Affymetrix protocol
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hrs at 45C on the Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using wash protocol EukGE-WS2v5.
| | Sample_scan_protocol | Arrays were scanned on a GeneChip® Scanner 3000 7G
| | Sample_data_processing | Raw cel files were imported into ArrayAssist 3.3 software (Stratagene, La Jolla, CA) and processed using GC-RMA, including model-based normalization.
| | Sample_platform_id | GPL201
| | Sample_contact_name | William,,Chang
| | Sample_contact_email | wlchang@ucdavis.edu
| | Sample_contact_phone | 530-752-6248
| | Sample_contact_fax | 530-752-7914
| | Sample_contact_department | Center for Comparative Medicine
| | Sample_contact_institute | University of California, Davis
| | Sample_contact_address | County Rd 98 and Hutchison Dr
| | Sample_contact_city | Davis
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 95616
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM170nnn/GSM170704/suppl/GSM170704.CEL.gz
| | Sample_series_id | GSE7095
| | Sample_data_row_count | 8793
| | |
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