Search results for the GEO ID: GSE7271 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM175440 | GPL341 |
|
Female lung, uninfected (rep1)
|
Female lung, uninfected (rep1)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175440
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175440/suppl/GSM175440.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175441 | GPL341 |
|
Female lung, uninfected (rep2)
|
Female lung, uninfected (rep2)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175441
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175441/suppl/GSM175441.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175442 | GPL341 |
|
Female lung, uninfected (rep3)
|
Female lung, uninfected (rep3)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175442
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175442/suppl/GSM175442.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175443 | GPL341 |
|
Female lung, infected, 3 days p.i. (rep1)
|
Female lung, infected, 3 days p.i. (rep1)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175443
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175443/suppl/GSM175443.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175444 | GPL341 |
|
Female lung, infected, 3 days p.i. (rep2)
|
Female lung, infected, 3 days p.i. (rep2)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175444
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175444/suppl/GSM175444.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175445 | GPL341 |
|
Female lung, infected, 3 days p.i. (rep3)
|
Female lung, infected, 3 days p.i. (rep3)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175445
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175445/suppl/GSM175445.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175446 | GPL341 |
|
Female lung, infected, 15 days p.i. (rep1)
|
Female lung, infected, 15 days p.i. (rep1)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175446
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175446/suppl/GSM175446.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175447 | GPL341 |
|
Female lung, infected, 15 days p.i. (rep2)
|
Female lung, infected, 15 days p.i. (rep2)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175447
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175447/suppl/GSM175447.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175448 | GPL341 |
|
Female lung, infected, 15 days p.i. (rep3)
|
Female lung, infected, 15 days p.i. (rep3)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175448
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175448/suppl/GSM175448.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175449 | GPL341 |
|
Female lung, infected, 40 days p.i. (rep1)
|
Female lung, infected, 40 days p.i. (rep1)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175449
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175449/suppl/GSM175449.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175450 | GPL341 |
|
Female lung, infected, 40 days p.i. (rep2)
|
Female lung, infected, 40 days p.i. (rep2)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175450
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175450/suppl/GSM175450.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175451 | GPL341 |
|
Female lung, infected, 40 days p.i. (rep3)
|
Female lung, infected, 40 days p.i. (rep3)
|
Strain: Long Evans; Sex: Female; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175451
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175451/suppl/GSM175451.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175452 | GPL341 |
|
Male lung, uninfected (rep1)
|
Male lung, uninfected (rep1)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175452
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175452/suppl/GSM175452.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175453 | GPL341 |
|
Male lung, uninfected (rep2)
|
Male lung, uninfected (rep2)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175453
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175453/suppl/GSM175453.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175454 | GPL341 |
|
Male lung, uninfected (rep3)
|
Male lung, uninfected (rep3)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: uninfected
|
sham = gonadally intact
|
Sample_geo_accession | GSM175454
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175454/suppl/GSM175454.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175455 | GPL341 |
|
Male lung, infected, 3 days p.i. (rep1)
|
Male lung, infected, 3 days p.i. (rep1)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175455
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175455/suppl/GSM175455.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175456 | GPL341 |
|
Male lung, infected, 3 days p.i. (rep2)
|
Male lung, infected, 3 days p.i. (rep2)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175456
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175456/suppl/GSM175456.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175457 | GPL341 |
|
Male lung, infected, 3 days p.i. (rep3)
|
Male lung, infected, 3 days p.i. (rep3)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 3 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175457
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175457/suppl/GSM175457.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175458 | GPL341 |
|
Male lung, infected, 15 days p.i. (rep1)
|
Male lung, infected, 15 days p.i. (rep1)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175458
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175458/suppl/GSM175458.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175459 | GPL341 |
|
Male lung, infected, 15 days p.i. (rep2)
|
Male lung, infected, 15 days p.i. (rep2)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175459
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175459/suppl/GSM175459.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175460 | GPL341 |
|
Male lung, infected, 15 days p.i. (rep3)
|
Male lung, infected, 15 days p.i. (rep3)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 15 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175460
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175460/suppl/GSM175460.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175461 | GPL341 |
|
Male lung, infected, 40 days p.i. (rep1)
|
Male lung, infected, 40 days p.i. (rep1)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175461
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175461/suppl/GSM175461.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
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GSM175462 | GPL341 |
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Male lung, infected, 40 days p.i. (rep2)
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Male lung, infected, 40 days p.i. (rep2)
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Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
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sham = gonadally intact
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Sample_geo_accession | GSM175462
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175462/suppl/GSM175462.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
|
GSM175463 | GPL341 |
|
Male lung, infected, 40 days p.i. (rep3)
|
Male lung, infected, 40 days p.i. (rep3)
|
Strain: Long Evans; Sex: Male; Age: >80 days; Tissue: Lungs; Infection Status: 40 days p.i. with Seoul virus
|
sham = gonadally intact
|
Sample_geo_accession | GSM175463
| Sample_status | Public on Oct 30 2007
| Sample_submission_date | Mar 14 2007
| Sample_last_update_date | Oct 30 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA was extracted from individual lungs homogenized in TRIZOL. Pools of 2 individuals' RNA were subsequently prepared and processed, creating 3 separate pools per treatment group.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | 7.5 ug pooled total RNA was processed according to the Affymetrix Technical manual Rev3 protocol. Biotinylated cRNA was prepared using the ENZO BioArray High Yield RNA Transcript Labeling Kit.
| Sample_hyb_protocol | 10ug fragmented, biotinylated cRNA was hybridized to RAE230A GeneChips for 16hours at 45oC and 60rpm. The signal amplification protocol for washing/staining was performed in a FS450 automated fluidics station according to Affymetrix protocol.
| Sample_scan_protocol | GeneChips were scanned in an Affymetrix GCS3000 scanner with autoloader.
| Sample_data_processing | The data were analyzed with GCOS 1.4 software. All probe sets scaling was performed with a target signal value of 500. No normalization was performed.
| Sample_platform_id | GPL341
| Sample_contact_name | Sabra,Lynn,Klein
| Sample_contact_email | saklein@jhsph.edu
| Sample_contact_phone | (410) 955-8898
| Sample_contact_fax | (410) 955-0105
| Sample_contact_laboratory | Lab of Sabra Klein
| Sample_contact_department | Molecular Microbiology and Immunology
| Sample_contact_institute | Johns Hopkins Bloomberg School of Public Health
| Sample_contact_address | 615 North Wolfe Street
| Sample_contact_city | Baltimore
| Sample_contact_state | MD
| Sample_contact_zip/postal_code | 21205
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM175nnn/GSM175463/suppl/GSM175463.CEL.gz
| Sample_series_id | GSE7271
| Sample_data_row_count | 15923
| |
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