Search results for the GEO ID: GSE7441 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM178539 | GPL1355 |
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Primary rat spinal cord astrocytes NonTG-A
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Spinal cord astrocytes NonTG
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Primary spinal cord astrocytes from non-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
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Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM178539
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Mar 29 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM178nnn/GSM178539/suppl/GSM178539.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM178nnn/GSM178539/suppl/GSM178539.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
| |
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GSM180126 | GPL1355 |
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Primary rat spinal cord astrocytes NonTG-B
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Spinal cord astrocytes NonTG
|
Primary spinal cord astrocytes from non-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM180126
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Apr 03 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180126/suppl/GSM180126.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180126/suppl/GSM180126.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
| |
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GSM180128 | GPL1355 |
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Primary rat spinal cord astrocytes NonTG-C
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Spinal cord astrocytes NonTG
|
Primary spinal cord astrocytes from non-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM180128
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Apr 03 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180128/suppl/GSM180128.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180128/suppl/GSM180128.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
| |
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GSM180130 | GPL1355 |
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Primary rat spinal cord astrocytes hSOD1G93A-A
|
Spinal cord astrocytes from transgenic hSOD1G93A
|
Primary spinal cord astrocytes from hSOD1G93A-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM180130
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Apr 03 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180130/suppl/GSM180130.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180130/suppl/GSM180130.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
| |
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GSM180131 | GPL1355 |
|
Primary rat spinal cord astrocytes hSOD1G93A-B
|
Spinal cord astrocytes from transgenic hSOD1G93A
|
Primary spinal cord astrocytes from hSOD1G93A-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM180131
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Apr 03 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180131/suppl/GSM180131.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180131/suppl/GSM180131.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
| |
|
GSM180175 | GPL1355 |
|
Primary rat spinal cord astrocytes hSOD1G93A-C
|
Spinal cord astrocytes from transgenic hSOD1G93A
|
Primary spinal cord astrocytes from hSOD1G93A-transgenic 1 day old rat littermates obtained from the breeding of a hSOD1G93A positive male with a negative female.
Astrocytes were prepared as described in Cassina P, et al.J Neurosci Res. 2002;67(1):21-9.
Transgenic hSOD1G93A rats were provided by D. S. Howland (Howland et al. 2002; PNAS, 99(3): 1604-1609)
|
Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
5 ug of total RNA were used for the amplification.
Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5.
|
Sample_geo_accession | GSM180175
| Sample_status | Public on Dec 18 2008
| Sample_submission_date | Apr 03 2007
| Sample_last_update_date | Dec 18 2008
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Total RNA extracted with RNeasy Mini (Qiagen)
| Sample_label_ch1 | Biotin
| Sample_label_protocol_ch1 | Labeling was done with the Affymetrix kits according to their one-cycle target labeling assay. Kits used were the one-cycle cDNA synthesis kit(900431), sample cleanup modules(900371), eukaryotic Poly-A RNA control kit (900433), IVT labeling kit(900449), Eukayotic hybridization control kit(900454)
| Sample_hyb_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_scan_protocol | Hybridization, washing and scanning was done according to the Affymetrix protocol in GeneChip Expression Analysis Technical Manual 701021 Rev. 5
| Sample_data_processing | MAS5.0 (GCOS 1.4)
| Sample_data_processing | Alpha1 (0.05000000); Alpha2 (0.06500000); Tau (0.01500000); TGT (500)
| Sample_platform_id | GPL1355
| Sample_contact_name | Marcelo,R.,Vargas
| Sample_contact_email | vargasm@iibce.edu.uy
| Sample_contact_department | Neurobiología Celular y Molecular
| Sample_contact_institute | Instituto Clemente Estable
| Sample_contact_address | Av. Italia 3318
| Sample_contact_city | Montevideo
| Sample_contact_zip/postal_code | 11600
| Sample_contact_country | Uruguay
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180175/suppl/GSM180175.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180175/suppl/GSM180175.CHP.gz
| Sample_series_id | GSE7441
| Sample_data_row_count | 31099
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