Result

Search results for the GEO ID: GSE7479

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GSM ID

GPL ID

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Title

Source name

Description

Characteristics

GSM180920

GPL1355

Control, sample 1

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Control, sample 1

Sample_geo_accession	GSM180920
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 06 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180920/suppl/GSM180920.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM180nnn/GSM180920/suppl/GSM180920.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181037

GPL1355

Control, sample 2

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Control, sample 2

Sample_geo_accession	GSM181037
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181037/suppl/GSM181037.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181037/suppl/GSM181037.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181038

GPL1355

Control, sample 3

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Control, sample 3

Sample_geo_accession	GSM181038
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181038/suppl/GSM181038.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181038/suppl/GSM181038.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181039

GPL1355

Control, sample 4

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Control, sample 4

Sample_geo_accession	GSM181039
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181039/suppl/GSM181039.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181039/suppl/GSM181039.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181040

GPL1355

Quercetin, sample 1

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Quercetin, sample 1

Sample_geo_accession	GSM181040
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181040/suppl/GSM181040.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181040/suppl/GSM181040.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181041

GPL1355

Quercetin, sample 2

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Quercetin, sample 2

Sample_geo_accession	GSM181041
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181041/suppl/GSM181041.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181041/suppl/GSM181041.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181042

GPL1355

Quercetin, sample 3

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Quercetin, sample 3

Sample_geo_accession	GSM181042
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181042/suppl/GSM181042.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181042/suppl/GSM181042.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

GSM181043

GPL1355

Quercetin, sample 4

Scrapings of the distal colon mucosa

Male F344 rats, sacrificed at the age 18 weeks

Quercetin, sample 4

Sample_geo_accession	GSM181043
Sample_status	Public on Jan 01 2008
Sample_submission_date	Apr 09 2007
Sample_last_update_date	Nov 30 2007
Sample_type	RNA
Sample_channel_count	1
Sample_organism_ch1	Rattus norvegicus
Sample_taxid_ch1	10116
Sample_biomaterial_provider_ch1	Charles River Breeding Laboratory, Someren, The Netherlands
Sample_treatment_protocol_ch1	During section, the distal colon was rinsed with 70% ethanol at 4°C, subsequently scraped with sterilized glass slides and fixed in RNAlater® (Ambion®, Cambridgeshire, U.K.) according to the manufacturer’s protocol.
Sample_growth_protocol_ch1	Rats were fed either 10 g quercetin/kg diet or the control diet(RM3 [E] FG SQC rat breeder diet obtained from SDS Special Diets Services, Witham, England) for 11 weeks.
Sample_molecule_ch1	total RNA
Sample_extract_protocol_ch1	RNA extraction was performed with the TRIzol Reagent (Life Technologies, Paisley, U.K.), as described by the manufacturer.
Sample_extract_protocol_ch1	Total RNA was purified with the NucleoSpin® RNA II kit (Machery-Nagel, Düren, Germany), including a DNAse incubation step to digest possible traces of DNA. Subsequent analysis on the Agilent 2100 bioanalyser (Agilent Technologies, Palo Alto, California, U.S.A.) indicated high quality total RNA (28S/18S ribosomal RNA ratios ~2.0) that was suitable for hybridization
Sample_label_ch1	Biotin
Sample_label_protocol_ch1	For RNA labeling, GeneChip® One-Cycle Eukaryotic Target Labeling Assay for expression analysis was performed, as described in the Affymetrix® GeneChip® Expression Analysis Technical Manual
Sample_hyb_protocol	Hybridisation on Affymetrix® GeneChip® arrays was processed according to the GeneChip® One-Cycle Eukaryotic Target Labeling Assay.
Sample_scan_protocol	Affymetrix® GeneChip® arrays were scanned with the GeneChip® Scanner 3000 7G (Affymetrix® Inc., Santa Clara, California, U.S.A.).
Sample_data_processing	Data were processed with MAS 5.0 and a scaling factor of 200 was applied.
Sample_platform_id	GPL1355
Sample_contact_name	Ashwin,,Dihal
Sample_contact_email	rob.stierum@tno.nl
Sample_contact_phone	+31 (0)30-6944545
Sample_contact_fax	+31 (0)30 6944989
Sample_contact_department	Business Unit Physiologival Sciences
Sample_contact_institute	TNO Quality of Life
Sample_contact_address	Utrechtseweg 48
Sample_contact_city	Zeist
Sample_contact_zip/postal_code	3704 HE
Sample_contact_country	Netherlands
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181043/suppl/GSM181043.CEL.gz
Sample_supplementary_file	ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181043/suppl/GSM181043.CHP.gz
Sample_series_id	GSE7479
Sample_data_row_count	31099

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