Search results for the GEO ID: GSE7491 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM181524 | GPL1355 |
|
postnatal day 2, biological repl1 (litter 1)
|
Lung fibroblasts isolated on postnatal day 2, biological repl1 (litter 1)
|
Sprague Dawley
pooled cells from 5 pups (2-day old) from litter 1
|
pooled cells from 5 pups (2-day old, before septation) from litter 1
|
Sample_geo_accession | GSM181524
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181524/suppl/GSM181524.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181525 | GPL1355 |
|
postnatal day 2, biological repl2 (litter 2)
|
Lung fibroblasts isolated on postnatal day 2, biological repl2 (litter 2)
|
Sprague Dawley
pooled cells from 5 pups (2-day old) from litter 2
|
pooled cells from 5 pups (2-day old, before septation) from litter 2
|
Sample_geo_accession | GSM181525
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181525/suppl/GSM181525.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181526 | GPL1355 |
|
postnatal day 2, biological repl3 (litter 3)
|
Lung fibroblasts isolated on postnatal day 2, biological repl3 (litter 3)
|
Sprague Dawley
pooled cells from 5 pups (2-day old) from litter 3
|
pooled cells from 5 pups (2-day old, before septation) from litter 3
|
Sample_geo_accession | GSM181526
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181526/suppl/GSM181526.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181527 | GPL1355 |
|
postnatal day 7, biological repl1 (litter 1)
|
Lung fibroblasts isolated on postnatal day 7, biological repl1 (litter 1)
|
Sprague Dawley
pooled cells from 4 pups (7-day old) from litter 1
|
pooled cells from 4 pups (7-day old, progressing secondary septation) from litter 1
|
Sample_geo_accession | GSM181527
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181527/suppl/GSM181527.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181528 | GPL1355 |
|
postnatal day 7, biological repl2 (litter 2)
|
Lung fibroblasts isolated on postnatal day 7, biological repl2 (litter 2)
|
Sprague Dawley
pooled cells from 4 pups (7-day old) from litter 2
|
pooled cells from 4 pups (7-day old, progressing secondary septation) from litter 2
|
Sample_geo_accession | GSM181528
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181528/suppl/GSM181528.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181529 | GPL1355 |
|
postnatal day 7, biological repl3 (litter 3)
|
Lung fibroblasts isolated on postnatal day 7, biological repl3 (litter 3)
|
Sprague Dawley
pooled cells from 4 pups (7-day old) from litter 3
|
pooled cells from 4 pups (7-day old, progressing secondary septation) from litter 3
|
Sample_geo_accession | GSM181529
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181529/suppl/GSM181529.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181530 | GPL1355 |
|
postnatal day 21, biological repl1 (litter 1)
|
Lung fibroblasts isolated on postnatal day 21, biological repl1 (litter 1)
|
Sprague Dawley
pooled cells from 4 pups (21-day old) from litter 1
|
pooled cells from 4 pups (21-day old, terminated secondary septation) from litter 1
|
Sample_geo_accession | GSM181530
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181530/suppl/GSM181530.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181531 | GPL1355 |
|
postnatal day 21, biological repl2 (litter 2)
|
Lung fibroblasts isolated on postnatal day 21, biological repl2 (litter 2)
|
Sprague Dawley
pooled cells from 4 pups (21-day old) from litter 2
|
pooled cells from 4 pups (21-day old, terminated secondary septation) from litter 2
|
Sample_geo_accession | GSM181531
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181531/suppl/GSM181531.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
GSM181532 | GPL1355 |
|
postnatal day 21, biological repl3 (litter 3)
|
Lung fibroblasts isolated on postnatal day 21, biological repl3 (litter 3)
|
Sprague Dawley
pooled cells from 4 pups (21-day old) from litter 3
|
pooled cells from 4 pups (21-day old, terminated secondary septation) from litter 3
|
Sample_geo_accession | GSM181532
| Sample_status | Public on Apr 12 2007
| Sample_submission_date | Apr 10 2007
| Sample_last_update_date | Apr 11 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Rattus norvegicus
| Sample_taxid_ch1 | 10116
| Sample_treatment_protocol_ch1 | Lung cells were dispersed by trypsin and collagenase, then plated onto plastic Petri dishes in MEM-10% FBS and allowed to adhere for 45 min. Non-adherent cells were discarded, and the adherent fibroblasts were rinsed and immediately scrapped and pelleted. Freshly isolated cells were stored at -80°C until RNA extraction.
| Sample_growth_protocol_ch1 | Rat lung fibroblasts were extemporaneously isolated on postnatal days 2 (saccular stage), 7 (alveolar stage, progressing secondary septation) and 21 (alveolar stage, terminated secondary septation)
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 10 µg cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneArray Scanner 3000
| Sample_data_processing | The data were obtained by processing raw CEL files through the Affy package. Background correction method RMA, normalization method qspline, probe-level correction Pmonly, and summarization method Li-Wong model were used.
| Sample_platform_id | GPL1355
| Sample_contact_name | Jacques,,Bourbon
| Sample_contact_email | jacques.bourbon@creteil.inserm.fr
| Sample_contact_phone | 00 33 1 49 81 37 33
| Sample_contact_fax | 00 33 1 49 98 37 33
| Sample_contact_department | Faculté de Médecine
| Sample_contact_institute | Université de Paris 12
| Sample_contact_address | 8 rue du Général Sarrail
| Sample_contact_city | Créteil
| Sample_contact_zip/postal_code | 94000
| Sample_contact_country | France
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181532/suppl/GSM181532.CEL.gz
| Sample_series_id | GSE7491
| Sample_data_row_count | 31099
| |
|
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