Search results for the GEO ID: GSE7501  |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM181825 | GPL341 |
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RCG-12 cells cultured at permissive temperature for 3 days-1
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Rat astrocyte cell line RCG-12
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Rat astrocyte cell line RCG-12
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A rat conditionally immortalized astrocyte cell line, RCG-12, derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen was used. RCG-12 cells were cultured at permissive temperature (33°C) for 3 days.
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| Sample_geo_accession | GSM181825
| | Sample_status | Public on Apr 13 2007
| | Sample_submission_date | Apr 12 2007
| | Sample_last_update_date | Apr 12 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181825/suppl/GSM181825.CEL.gz
| | Sample_series_id | GSE7501
| | Sample_data_row_count | 15923
| | |
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GSM181826 | GPL341 |
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RCG-12 cells cultured at permissive temperature for 3 days-2
|
Rat astrocyte cell line RCG-12
|
Rat astrocyte cell line RCG-12
|
A rat conditionally immortalized astrocyte cell line, RCG-12, derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen was used. RCG-12 cells were cultured at permissive temperature (33°C) for 3 days.
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| Sample_geo_accession | GSM181826
| | Sample_status | Public on Apr 13 2007
| | Sample_submission_date | Apr 12 2007
| | Sample_last_update_date | Apr 12 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181826/suppl/GSM181826.CEL.gz
| | Sample_series_id | GSE7501
| | Sample_data_row_count | 15923
| | |
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GSM181827 | GPL341 |
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RCG-12 cells cultured at nonpermissive temperature for 3 days-1
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Rat astrocyte cell line RCG-12
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Rat astrocyte cell line RCG-12
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A rat conditionally immortalized astrocyte cell line, RCG-12, derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen was used. RCG-12 cells were cultured at nonpermissive temperature (39°C) for 3 days.
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| Sample_geo_accession | GSM181827
| | Sample_status | Public on Apr 13 2007
| | Sample_submission_date | Apr 12 2007
| | Sample_last_update_date | Apr 12 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181827/suppl/GSM181827.CEL.gz
| | Sample_series_id | GSE7501
| | Sample_data_row_count | 15923
| | |
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GSM181828 | GPL341 |
|
RCG-12 cells cultured at nonpermissive temperature for 3 days-2
|
Rat astrocyte cell line RCG-12
|
Rat astrocyte cell line RCG-12
|
A rat conditionally immortalized astrocyte cell line, RCG-12, derived from primary cultured rat cortical glia cells infecting with a temperature-sensitive simian virus 40 large T-antigen was used. RCG-12 cells were cultured at nonpermissive temperature (39°C) for 3 days.
|
| Sample_geo_accession | GSM181828
| | Sample_status | Public on Apr 13 2007
| | Sample_submission_date | Apr 12 2007
| | Sample_last_update_date | Apr 12 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA was extracted from the cells using an RNeasy Total RNA Extraction Kit (Qiagen K.K., Tokyo, Japan). Then, RNA samples were treated with RNase-free DNase.
| | Sample_label_ch1 | phycoerythrin
| | Sample_label_protocol_ch1 | 5 μg of total RNA was used to synthesize double-strand cDNA with a GeneChip® Expression 3’-Amplification Reagents One-Cycle cDNA Synthesis Kit (Affymetrix). Biotin-labeled cRNA was then synthesized from the cDNA using GeneChip® Expression 3’-Amplification Reagents for IVT Labeling (Affymetrix).
| | Sample_hyb_protocol | After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
| | Sample_scan_protocol | The arrays were washed, stained with streptavidin-phycoerythrin and scanned with a probe array scanner (Affymetrix).
| | Sample_data_processing | GeneChip Analysis Suite
| | Sample_platform_id | GPL341
| | Sample_contact_name | Yoshiaki,,Tabuchi
| | Sample_contact_email | ytabu@cts.u-toyama.ac.jp
| | Sample_contact_laboratory | Division of Molecular Genetics Research
| | Sample_contact_department | Life Science Research Center
| | Sample_contact_institute | University of Toyama
| | Sample_contact_address | 2630 Sugitani
| | Sample_contact_city | Toyama
| | Sample_contact_state | Toyama
| | Sample_contact_zip/postal_code | 930-0194
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM181nnn/GSM181828/suppl/GSM181828.CEL.gz
| | Sample_series_id | GSE7501
| | Sample_data_row_count | 15923
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