Search results for the GEO ID: GSE7596  |
(Click on the check boxes provided under "Select for analysis", to initiate grouping) |
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GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM176890 | GPL1261 |
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AktTGF, rep1
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Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3455.CEL
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GSM176891 | GPL1261 |
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GFPTGF, rep1
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Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3459.CEL
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GSM180510 | GPL1261 |
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AktTGF, rep2
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Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3458.CEL
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GSM180513 | GPL1261 |
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AktTGF, rep3
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Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3453.CEL
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GSM180515 | GPL1261 |
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GFPTGF, rep2
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Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3452.CEL
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GSM180516 | GPL1261 |
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GFPTGF, rep3
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Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
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Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
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jos3457.CEL
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