Search results for the GEO ID: GSE7637  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM184636 | GPL570 |
|
MSC #5, technical rep-amplification- rep1
|
mesenchymal stem cells, 5th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 5
|
| Sample_geo_accession | GSM184636
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184636/suppl/GSM184636.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184637 | GPL570 |
|
MSC #5, technical rep-amplification- rep2
|
mesenchymal stem cells, 5th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 5
|
| Sample_geo_accession | GSM184637
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184637/suppl/GSM184637.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184638 | GPL570 |
|
MSC #5, technical rep-amplification- rep3
|
mesenchymal stem cells, 5th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 5
|
| Sample_geo_accession | GSM184638
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184638/suppl/GSM184638.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184639 | GPL570 |
|
MSC #7, technical rep-extract-rep1
|
mesenchymal stem cells, 7th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184639
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184639/suppl/GSM184639.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184640 | GPL570 |
|
MSC #7, technical rep-extract-rep2
|
mesenchymal stem cells, 7th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184640
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184640/suppl/GSM184640.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184641 | GPL570 |
|
MSC #7, technical rep-extract-rep3
|
mesenchymal stem cells, 7th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184641
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184641/suppl/GSM184641.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184642 | GPL570 |
|
MSC #9, technical rep-extract-rep1
|
mesenchymal stem cells, 9th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184642
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184642/suppl/GSM184642.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184643 | GPL570 |
|
MSC #9, technical rep-extract-rep2
|
mesenchymal stem cells, 9th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184643
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184643/suppl/GSM184643.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184644 | GPL570 |
|
MSC #9, technical rep-extract-rep3
|
mesenchymal stem cells, 9th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184644
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184644/suppl/GSM184644.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184645 | GPL570 |
|
MSC #13, technical rep-extract-rep1
|
mesenchymal stem cells, 13th passage
|
one-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 13
|
| Sample_geo_accession | GSM184645
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184645/suppl/GSM184645.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184646 | GPL570 |
|
MSC #13, technical rep-extract-rep2
|
mesenchymal stem cells, 13th passage
|
one-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 13
|
| Sample_geo_accession | GSM184646
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184646/suppl/GSM184646.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184647 | GPL570 |
|
MSC #13, technical rep-extract-rep3
|
mesenchymal stem cells, 13th passage
|
one-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 13
|
| Sample_geo_accession | GSM184647
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184647/suppl/GSM184647.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184648 | GPL570 |
|
MSC #14
|
mesenchymal stem cells, 14th passage
|
one-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 14
|
| Sample_geo_accession | GSM184648
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184648/suppl/GSM184648.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184649 | GPL570 |
|
MSC #21, technical rep-extract-rep1
|
mesenchymal stem cells, 21st passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 21
|
| Sample_geo_accession | GSM184649
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184649/suppl/GSM184649.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184650 | GPL570 |
|
MSC #21, technical rep-extract-rep2
|
mesenchymal stem cells, 21st passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 21
|
| Sample_geo_accession | GSM184650
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184650/suppl/GSM184650.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184651 | GPL570 |
|
MSC #28, technical rep-extract-rep1
|
mesenchymal stem cells, 28th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 28
|
| Sample_geo_accession | GSM184651
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184651/suppl/GSM184651.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184652 | GPL570 |
|
MSC #28, technical rep-extract-rep2
|
mesenchymal stem cells, 28th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 28
|
| Sample_geo_accession | GSM184652
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184652/suppl/GSM184652.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184653 | GPL570 |
|
MSC #28, technical rep-extract-rep3
|
mesenchymal stem cells, 28th passage
|
two-cycle
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 28
|
| Sample_geo_accession | GSM184653
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184653/suppl/GSM184653.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184654 | GPL570 |
|
MSC #7, technical rep-label-rep1
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184654
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184654/suppl/GSM184654.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184655 | GPL570 |
|
MSC #7, technical rep-label-rep2
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184655
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184655/suppl/GSM184655.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184656 | GPL570 |
|
MSC #7, technical rep-label-rep3
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184656
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184656/suppl/GSM184656.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184657 | GPL570 |
|
MSC #7, technical rep-label-rep4
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184657
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184657/suppl/GSM184657.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184658 | GPL570 |
|
MSC #7, technical rep-label-rep5
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184658
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184658/suppl/GSM184658.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184659 | GPL570 |
|
MSC #7, technical rep-label-rep6
|
mesenchymal stem cells, 7th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 7
|
| Sample_geo_accession | GSM184659
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184659/suppl/GSM184659.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184660 | GPL570 |
|
MSC #9, technical rep-label-rep1
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184660
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184660/suppl/GSM184660.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184661 | GPL570 |
|
MSC #9, technical rep-label-rep2
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184661
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184661/suppl/GSM184661.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184662 | GPL570 |
|
MSC #9, technical rep-label-rep3
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184662
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184662/suppl/GSM184662.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184663 | GPL570 |
|
MSC #9, technical rep-label-rep4
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184663
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184663/suppl/GSM184663.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184664 | GPL570 |
|
MSC #9, technical rep-label-rep5
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184664
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184664/suppl/GSM184664.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
GSM184665 | GPL570 |
|
MSC #9, technical rep-label-rep6
|
mesenchymal stem cells, 9th passage
|
started from 2nd cycle (step6) in two-cycle protocol
bone marrow mesenchymal stem cells
|
Gene expression data from mesenchymal stem cells cultured in MSCGM (mesenchymal stem cell growth medium), passage number 9
|
| Sample_geo_accession | GSM184665
| | Sample_status | Public on Jun 06 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | Jun 06 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | Cells were lysed in 600 ul of buffer RLT (QIAGEN).
| | Sample_growth_protocol_ch1 | Mesenchymal stem cells were cultured according to manufacturer's protocol (Lonza).
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Total RNA extraction was performed using silica-gel-based membrane according to manufacturer's protocol (QIAGEN, RNeasy)
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to Two-cycle or One-cycle Affymetrix protocol from 100 ng (Two-cycle) or 1ug (One-cycle) total RNA (Expression Analysis Technical Manual, 701024 Rev.3, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for aprx. 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChip array was scanned using the Affymetrix GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed using GCOS Affymetrix standard analysis settings and mask-file (Affymetrix, hgu133_plus_2norm) scaling as normalization methods. The trimmed mean target intensity of each array was arbitrarily set to 10000.
| | Sample_platform_id | GPL570
| | Sample_contact_name | Shihori,,Tanabe
| | Sample_contact_department | Division of Safety Information on Drug, Food and Chemicals
| | Sample_contact_institute | National Institute of Health Sciences
| | Sample_contact_address | 1-18-1, Kami-yoga,
| | Sample_contact_city | Setagaya-ku
| | Sample_contact_state | Tokyo
| | Sample_contact_zip/postal_code | 158-8501
| | Sample_contact_country | Japan
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184665/suppl/GSM184665.CEL.gz
| | Sample_series_id | GSE7637
| | Sample_data_row_count | 54675
| | |
|
| |
| |
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(2 groups will be compared at a time) |
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