Search results for the GEO ID: GSE7640  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM184851 | GPL1355 |
|
Sedentary rat 1
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184851
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184851/suppl/GSM184851.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184851/suppl/GSM184851.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184852 | GPL1355 |
|
Sedentary rat 2
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184852
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184852/suppl/GSM184852.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184852/suppl/GSM184852.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184853 | GPL1355 |
|
Sedentary rat 3
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184853
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184853/suppl/GSM184853.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184853/suppl/GSM184853.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184854 | GPL1355 |
|
Sedentary rat 4
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184854
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184854/suppl/GSM184854.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184854/suppl/GSM184854.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184855 | GPL1355 |
|
Sedentary rat 5
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184855
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184855/suppl/GSM184855.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184855/suppl/GSM184855.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184856 | GPL1355 |
|
Sedentary rat 6
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184856
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184856/suppl/GSM184856.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184856/suppl/GSM184856.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184857 | GPL1355 |
|
Sedentary rat 7
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184857
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184857/suppl/GSM184857.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184857/suppl/GSM184857.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184858 | GPL1355 |
|
Sedentary rat 8
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184858
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184858/suppl/GSM184858.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184858/suppl/GSM184858.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184859 | GPL1355 |
|
Sedentary rat 9
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184859
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184859/suppl/GSM184859.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184859/suppl/GSM184859.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184860 | GPL1355 |
|
Sedentary rat 10
|
Left ventricle of sedentary rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of sedentary rat
|
| Sample_geo_accession | GSM184860
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184860/suppl/GSM184860.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184860/suppl/GSM184860.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184861 | GPL1355 |
|
Exercised rat 1
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184861
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184861/suppl/GSM184861.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184861/suppl/GSM184861.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184862 | GPL1355 |
|
Exercised rat 2
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184862
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184862/suppl/GSM184862.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184862/suppl/GSM184862.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184863 | GPL1355 |
|
Exercised rat 3
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184863
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184863/suppl/GSM184863.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184863/suppl/GSM184863.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184864 | GPL1355 |
|
Exercised rat 4
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184864
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184864/suppl/GSM184864.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184864/suppl/GSM184864.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184865 | GPL1355 |
|
Exercised rat 5
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184865
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184865/suppl/GSM184865.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184865/suppl/GSM184865.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184866 | GPL1355 |
|
Exercised rat 6
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184866
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184866/suppl/GSM184866.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184866/suppl/GSM184866.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184867 | GPL1355 |
|
Exercised rat 7
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184867
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184867/suppl/GSM184867.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184867/suppl/GSM184867.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184868 | GPL1355 |
|
Exercised rat 8
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184868
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184868/suppl/GSM184868.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184868/suppl/GSM184868.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184869 | GPL1355 |
|
Exercised rat 9
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184869
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184869/suppl/GSM184869.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184869/suppl/GSM184869.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
GSM184870 | GPL1355 |
|
Exercised rat 10
|
Left ventricle of exercise-trained rat
|
Sprague-Dawley-CD rat (8 weeks old, 360±15 g body weight at the beginning of the study)
|
Gene expression data from left ventricle of exercise-trained rat
|
| Sample_geo_accession | GSM184870
| | Sample_status | Public on Jul 15 2008
| | Sample_submission_date | Apr 26 2007
| | Sample_last_update_date | May 16 2008
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Rattus norvegicus
| | Sample_taxid_ch1 | 10116
| | Sample_growth_protocol_ch1 | We used 20 male Sprague–Dawley-CD rats (8 weeks old, 360±15 g body weight at the beginning of the study), randomly divided into two groups: sedentary- controls and exercise-trained animals. During the experimental period, all animals had free access to water and conventional laboratory diet (Standard diet n.48, Laboratorio Dottori Piccioni,Gessate, Milan, Italy) until sacrifice. Room temperature was kept at 21 ± 2°C and 12 h of light were automatically alternated to 12 h of dark.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Trizol extraction of total RNA was performed according to the manufacturer's instructions.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug of total RNA (Expression Analysis Technical Manual, 2005, Affymetrix).
| | Sample_hyb_protocol | Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| | Sample_scan_protocol | GeneChips were scanned using the GeneChip Scanner 3000.
| | Sample_data_processing | The data were analyzed with Bioconductor Packages.
| | Sample_platform_id | GPL1355
| | Sample_contact_name | Betti,,Giusti
| | Sample_contact_department | Dpt. of Medical and Surgical Critical Care
| | Sample_contact_institute | University of Florence
| | Sample_contact_address | viale Morgagni, 85
| | Sample_contact_city | Florence
| | Sample_contact_zip/postal_code | 50134
| | Sample_contact_country | Italy
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184870/suppl/GSM184870.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM184nnn/GSM184870/suppl/GSM184870.CHP.gz
| | Sample_series_id | GSE7640
| | Sample_data_row_count | 31099
| | |
|
| |
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