Search results for the GEO ID: GSE7708  |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM187369 | GPL570 |
|
No_DHT_control_1
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 1
|
cultured LNCaP cells
refer to ATTC
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM187369
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187369/suppl/GSM187369.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187369/suppl/GSM187369.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187370 | GPL570 |
|
No_DHT_control_2
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 2
|
cultured LNCaP cells
refer to ATTC
|
Untreated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM187370
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187370/suppl/GSM187370.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187370/suppl/GSM187370.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187371 | GPL570 |
|
Control_1
|
Untreated LNCaP cells stimulated with DHT, biological replicate 1
|
cultured LNCaP cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM187371
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187371/suppl/GSM187371.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187371/suppl/GSM187371.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187372 | GPL570 |
|
Control_2
|
Untreated LNCaP cells stimulated with DHT, biological replicate 2
|
cultured LNCaP cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM187372
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187372/suppl/GSM187372.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187372/suppl/GSM187372.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187373 | GPL570 |
|
Control_3
|
Untreated LNCaP cells stimulated with DHT, biological replicate 3
|
cultured LNCaP cells
refer to ATTC
|
Untreated LNCaP cells stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM187373
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187373/suppl/GSM187373.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187373/suppl/GSM187373.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187374 | GPL570 |
|
Bicalutamide_1
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 1
|
cultured LNCaP cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM187374
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187374/suppl/GSM187374.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187374/suppl/GSM187374.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187375 | GPL570 |
|
Bicalutamide_2
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 2
|
cultured LNCaP cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM187375
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187375/suppl/GSM187375.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187375/suppl/GSM187375.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187376 | GPL570 |
|
Bicalutamide_3
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 3
|
cultured LNCaP cells
refer to ATTC
|
Bicalutamide treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM187376
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187376/suppl/GSM187376.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187376/suppl/GSM187376.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187377 | GPL570 |
|
Polyamide1_1
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM187377
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187377/suppl/GSM187377.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187377/suppl/GSM187377.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187378 | GPL570 |
|
Polyamide1_2
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM187378
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187378/suppl/GSM187378.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187378/suppl/GSM187378.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187379 | GPL570 |
|
Polyamide1_3
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 1 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM187379
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187379/suppl/GSM187379.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187379/suppl/GSM187379.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187380 | GPL570 |
|
Polyamide2_1
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 1
|
| Sample_geo_accession | GSM187380
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187380/suppl/GSM187380.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187380/suppl/GSM187380.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187381 | GPL570 |
|
Polyamide2_2
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 2
|
| Sample_geo_accession | GSM187381
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187381/suppl/GSM187381.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187381/suppl/GSM187381.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
|
GSM187382 | GPL570 |
|
Polyamide2_3
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
cultured LNCaP cells
refer to ATTC
|
Polyamide 2 treated LNCaP cells not stimulated with DHT, biological replicate 3
|
| Sample_geo_accession | GSM187382
| | Sample_status | Public on May 05 2007
| | Sample_submission_date | May 04 2007
| | Sample_last_update_date | May 04 2007
| | Sample_type | RNA
| | Sample_channel_count | 1
| | Sample_organism_ch1 | Homo sapiens
| | Sample_taxid_ch1 | 9606
| | Sample_treatment_protocol_ch1 | After the 2-day growth, media was changed to 10% charcoal-stripped FBS RPMI and at this time 10 microM of polyamide targeted to the ARE or 10 microM of a control polyamide were added. Cells were grown for 48 hours more. Bicalutamide (10 microM) was added to the appropriate cells, incubataed for 2 hours. DHT was added (1 nM) for 16 hours.
| | Sample_growth_protocol_ch1 | LNCaP cells seeded in 12-well plates at 90-100,000 cells per plate in 10% FBS RPMI for 3 days.
| | Sample_molecule_ch1 | total RNA
| | Sample_extract_protocol_ch1 | Cells were harvested for RNA using the Qiagen RNEasy kit and protocol. Control cells that were not treated with DHT were also harvested.
| | Sample_label_ch1 | biotin
| | Sample_label_protocol_ch1 | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_hyb_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_scan_protocol | Standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
| | Sample_data_processing | Resolver (Rosetta Biosoftware, Seattle)
| | Sample_platform_id | GPL570
| | Sample_contact_name | Peter,,Dervan
| | Sample_contact_institute | California Institute of Technology
| | Sample_contact_address | 1200 East California Blvd
| | Sample_contact_city | Pasadena
| | Sample_contact_state | CA
| | Sample_contact_zip/postal_code | 91125
| | Sample_contact_country | USA
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187382/suppl/GSM187382.CEL.gz
| | Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM187nnn/GSM187382/suppl/GSM187382.CHP.gz
| | Sample_series_id | GSE7708
| | Sample_data_row_count | 54675
| | |
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