Search results for the GEO ID: GSE7768 |
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|
GSM ID | GPL ID |
Select for analysis |
Title |
Source name |
Description |
Characteristics |
GSM188270 | GPL339 |
|
Ova, biological replicate 1
|
Whole mouse spleen, 6h after immunization with Ova peptides
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides
|
Sample_geo_accession | GSM188270
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188270/suppl/GSM188270.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188270/suppl/GSM188270.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188271 | GPL339 |
|
Ova, biological replicate 2
|
Whole mouse spleen, 6h after immunization with Ova peptides
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides
|
Sample_geo_accession | GSM188271
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188271/suppl/GSM188271.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188271/suppl/GSM188271.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188272 | GPL339 |
|
Ova, biological replicate 3
|
Whole mouse spleen, 6h after immunization with Ova peptides
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides
|
Sample_geo_accession | GSM188272
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188272/suppl/GSM188272.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188272/suppl/GSM188272.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188273 | GPL339 |
|
OvaLPS, biological replicate 1
|
Whole mouse spleen, 6h after immunization with Ova peptides plus LPS adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus LPS adjuvant
|
Sample_geo_accession | GSM188273
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188273/suppl/GSM188273.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188273/suppl/GSM188273.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188274 | GPL339 |
|
OvaLPS, biological replicate 2
|
Whole mouse spleen, 6h after immunization with Ova peptides plus LPS adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus LPS adjuvant
|
Sample_geo_accession | GSM188274
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188274/suppl/GSM188274.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188274/suppl/GSM188274.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188275 | GPL339 |
|
OvaLPS, biological replicate 3
|
Whole mouse spleen, 6h after immunization with Ova peptides plus LPS adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus LPS adjuvant
|
Sample_geo_accession | GSM188275
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188275/suppl/GSM188275.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188275/suppl/GSM188275.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188276 | GPL339 |
|
OvaMPL, biological replicate 1
|
Whole mouse spleen, 6h after immunization with Ova peptides plus MPL adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus MPL adjuvant
|
Sample_geo_accession | GSM188276
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188276/suppl/GSM188276.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188276/suppl/GSM188276.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188277 | GPL339 |
|
OvaMPL, biological replicate 2
|
Whole mouse spleen, 6h after immunization with Ova peptides plus MPL adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus MPL adjuvant
|
Sample_geo_accession | GSM188277
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188277/suppl/GSM188277.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188277/suppl/GSM188277.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
| |
|
GSM188278 | GPL339 |
|
OvaMPL, biological replicate 3
|
Whole mouse spleen, 6h after immunization with Ova peptides plus MPL adjuvant
|
Female B6.SJL mice, 9 weeks old at time of T cell transfer and immunization
|
Gene expression data from whole mouse spleen, 6h after injection with OVA peptides plus MPL adjuvant
|
Sample_geo_accession | GSM188278
| Sample_status | Public on Jun 09 2007
| Sample_submission_date | May 09 2007
| Sample_last_update_date | May 09 2007
| Sample_type | RNA
| Sample_channel_count | 1
| Sample_organism_ch1 | Mus musculus
| Sample_taxid_ch1 | 10090
| Sample_treatment_protocol_ch1 | Three treatments were given, by iv injection. "Ova": 10µg Ova257-264 + 50µg Ova323-339; "OvaLPS": Ova peptides + 10µg LPS from S. minnesota Re595 ('Ultrapure grade', Invivogen); "OvaMPL": ova peptides + 30µg MPL (manufactured from S. minnesota Re595 LPS, Invivogen). These doses of LPS and MPL were confirmed to have boosted to equal levels the clonal expansion of CD8+ OTI and CD4+ OTII T cells to stimulatory peptide; clonal expansion was tested in replicate mice on days 2.5 through 7 after immunization.
| Sample_growth_protocol_ch1 | B6.SJL female mice were infused with 4.7x10^5 B6.OTI plus 7x10^5 B6.OTII T cells by iv injection. Two days later, these recipient mice were given the treatments described below.
| Sample_molecule_ch1 | total RNA
| Sample_extract_protocol_ch1 | Spleens were removed from mice 6h after iv immunization and processed immediately using a Qiagen Rneasy total extraction kit, following the manufacturer's instructions.
| Sample_label_ch1 | biotin
| Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
| Sample_hyb_protocol | Following fragmentation, 15 µg of cRNA were hybridized for 16 hr at 45C on Affymetrix Genechip Mouse Expression array MOE430A. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
| Sample_scan_protocol | GeneChips were scanned using the Affymetrix GeneChip Scanner GCS 3000.
| Sample_data_processing | The data were analyzed with Array Assist 3 (Stratagene, La Jolla, CA) using their default algorithm for GC-RMA normalization. Data is displayed in linear space.
| Sample_platform_id | GPL339
| Sample_contact_name | Thomas,Clark,Mitchell
| Sample_contact_email | tom.mitchell@louisville.edu
| Sample_contact_phone | 502-852-2073
| Sample_contact_laboratory | Institute for Cellular Therapeutics
| Sample_contact_department | Microbiology and Immunology
| Sample_contact_institute | University of Louisville
| Sample_contact_address | 570 S. Preston St.
| Sample_contact_city | Louisville
| Sample_contact_state | KY
| Sample_contact_zip/postal_code | 40202
| Sample_contact_country | USA
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188278/suppl/GSM188278.CEL.gz
| Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188278/suppl/GSM188278.EXP.gz
| Sample_series_id | GSE7768
| Sample_data_row_count | 22690
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