Sample_geo_accession | GSM188288
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Sample_status | Public on Mar 14 2008
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Sample_submission_date | May 09 2007
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Sample_last_update_date | Mar 14 2008
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Sample_type | RNA
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Sample_channel_count | 1
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Sample_organism_ch1 | Mus musculus
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Sample_taxid_ch1 | 10090
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Sample_treatment_protocol_ch1 | infection with Streptococcus pyogenes
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Sample_growth_protocol_ch1 | ex vivo
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Sample_molecule_ch1 | total RNA
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Sample_extract_protocol_ch1 | peqGold TriFastTM (Peqlab)
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Sample_label_ch1 | biotin
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Sample_label_protocol_ch1 | Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Sample_hyb_protocol | Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Sample_scan_protocol | GeneChips were scanned using the Affymetrix GCS 3000.
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Sample_data_processing | The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
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Sample_platform_id | GPL339
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Sample_contact_name | Robert,,Geffers
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Sample_contact_email | robert.geffers@helmholtz-hzi.de
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Sample_contact_phone | +49 531-6181-3058
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Sample_contact_laboratory | Array Facility
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Sample_contact_department | Dep. Cell Biology
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Sample_contact_institute | HCI - Helmholtz Centre for Infection Research
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Sample_contact_address | Inhoffenstr. 7
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Sample_contact_city | Braunschweig
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Sample_contact_zip/postal_code | 38124
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Sample_contact_country | Germany
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Sample_supplementary_file | ftp://ftp.ncbi.nlm.nih.gov/geo/samples/GSM188nnn/GSM188288/suppl/GSM188288.CEL.gz
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Sample_series_id | GSE7769
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Sample_data_row_count | 22690
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